A ribonuclease T2 protein FocRnt2 contributes to the virulence of Fusarium oxysporum f. sp. cubense tropical race 4.

Publisher: Blackwell Science in collaboration with the British Society of Plant Pathology Country of Publication: England NLM ID: 100954969 Publication Model: Print Cited Medium: Internet ISSN: 1364-3703 (Electronic) Linking ISSN: 13643703 NLM ISO Abbreviation: Mol Plant Pathol Subsets: MEDLINE

Original Publication: Oxford, UK : Blackwell Science in collaboration with the British Society of Plant Pathology, c2000-

Fusarium*/pathogenicity ; Plant Diseases*/microbiology ; Musa*/microbiology ; Nicotiana*/microbiology; Virulence ; Fungal Proteins/metabolism ; Fungal Proteins/genetics ; Ribonucleases/metabolism ; Ribonucleases/genetics ; Reactive Oxygen Species/metabolism ; Endoribonucleases

Banana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), is a major disease of banana plants worldwide. Effector proteins play critical roles in banana-Foc TR4 interaction. Our previous studies highlighted a ribonuclease protein belonging to the T2 family (named as FocRnt2) in the Foc TR4 secretome, which was predicted to be an effector. However, its biological function in Foc TR4 infection is still unclear. Herein, we observed significant expression of FocRnt2 during the early stage of fungal infection in planta. A yeast signal sequence trap assay showed that FocRnt2 contained a functional signal peptide for secretion. FocRnt2 possessed ribonuclease activity that could degrade the banana total RNA in vitro. Subcellular localization showed that FocRnt2 was localized in the nucleus and cytoplasm of Nicotiana benthamiana leaves. Transient expression of FocRnt2 suppressed the expression of salicylic acid- and jasmonic acid-signalling marker genes, reactive oxygen species accumulation, and BAX-mediated cell death in N. benthamiana. FocRnt2 deletion limited fungal penetration, reduced fusaric acid biosynthesis in Foc TR4, and attenuated fungal virulence against banana plants, but had little effect on Foc TR4 growth and sensitivity to various stresses. Furthermore, FocRnt2 deletion mutants induced higher expression of the defence-related genes in banana plants. These results suggest that FocRnt2 plays an important role in full virulence of Foc TR4, further improving our understanding of effector-mediated Foc TR4 pathogenesis.
(© 2024 The Author(s). Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)

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CARS-31 China Agricultural Research System; 2022A1515140114 Basic and Applied Basic Research Foundation of Guangdong Province; 2023KJ134 Project for Key Technology R&D Innovation Team in Modern Agriculture; 32202381 National Natural Science Foundation of China; 31600663 National Natural Science Foundation of China

Keywords: Fusarium oxysporum f. sp. cubense; effector; ribonucleases T2; transient expression; virulence

0 (Fungal Proteins)
EC 3.1.- (Ribonucleases)
EC 3.1.27.1 (ribonuclease T(2))
0 (Reactive Oxygen Species)
EC 3.1.- (Endoribonucleases)

Fusarium oxysporum

Date Created: 20240808 Date Completed: 20240809 Latest Revision: 20240918

20240918

PMC11310096

10.1111/mpp.13502

39118198