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De novo gene synthesis by an antiviral reverse transcriptase.

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  • معلومة اضافية
    • المصدر:
      Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
    • بيانات النشر:
      Publication: : Washington, DC : American Association for the Advancement of Science
      Original Publication: New York, N.Y. : [s.n.] 1880-
    • الموضوع:
      DNA, Complementary*/biosynthesis ; DNA, Complementary*/genetics ; Klebsiella pneumoniae*/enzymology ; Klebsiella pneumoniae*/genetics ; Klebsiella pneumoniae*/virology ; Reverse Transcription* ; RNA, Untranslated*/genetics ; RNA, Untranslated*/metabolism ; RNA-Directed DNA Polymerase*/genetics ; RNA-Directed DNA Polymerase*/metabolism ; Siphoviridae*/genetics ; Siphoviridae*/growth & development; Open Reading Frames ; Templates, Genetic
    • نبذة مختصرة :
      Defense-associated reverse transcriptase (DRT) systems perform DNA synthesis to protect bacteria against viral infection, but the identities and functions of their DNA products remain largely unknown. We show that DRT2 systems encode an unprecedented immune pathway that involves de novo gene synthesis through rolling circle reverse transcription of a noncoding RNA (ncRNA). Programmed template jumping on the ncRNA generates a concatemeric cDNA, which becomes double-stranded upon viral infection. This DNA product constitutes a protein-coding, nearly endless open reading frame ( neo ) gene whose expression leads to potent cell growth arrest, restricting the viral infection. Our work highlights an elegant expansion of genome coding potential through RNA-templated gene creation and challenges conventional paradigms of genetic information encoded along the one-dimensional axis of genomic DNA.
    • Comments:
      Update of: bioRxiv. 2024 May 08:2024.05.08.593200. doi: 10.1101/2024.05.08.593200. (PMID: 38766058)
    • الرقم المعرف:
      0 (DNA, Complementary)
      0 (RNA, Untranslated)
      EC 2.7.7.49 (RNA-Directed DNA Polymerase)
    • الموضوع:
      Date Created: 20240808 Date Completed: 20241003 Latest Revision: 20241004
    • الموضوع:
      20241005
    • الرقم المعرف:
      10.1126/science.adq0876
    • الرقم المعرف:
      39116258