Impact of additives on syntrophic propionate and acetate enrichments under high-ammonia conditions.

Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE

Original Publication: Berlin ; New York : Springer International, c1984-

Propionates*/metabolism ; Ammonia*/metabolism ; Acetates*/metabolism ; Methane*/metabolism ; Zeolites*/chemistry ; Ferric Compounds*/metabolism; Graphite ; Anaerobiosis ; Bacteria/genetics ; Bacteria/metabolism ; Bacteria/drug effects ; Bacteria/classification ; Biofuels ; Biofilms/drug effects ; Biofilms/growth & development ; Bioreactors/microbiology

High ammonia concentrations in anaerobic degradation systems cause volatile fatty acid accumulation and reduced methane yield, which often derive from restricted activity of syntrophic acid-oxidising bacteria and hydrogenotrophic methanogens. Inclusion of additives that facilitate the electron transfer or increase cell proximity of syntrophic species by flocculation can be a suitable strategy to counteract these problems, but its actual impact on syntrophic interactions has yet to be determined. In this study, microbial cultivation and molecular and microscopic analysis were performed to evaluate the impact of conductive (graphene, iron oxide) and non-conductive (zeolite) additives on the degradation rate of acetate and propionate to methane by highly enriched ammonia-tolerant syntrophic cultures derived from a biogas process. All additives had a low impact on the lag phase but resulted in a higher rate of acetate (except graphene) and propionate degradation. The syntrophic bacteria 'Candidatus Syntrophopropionicum ammoniitolerans', Syntrophaceticus schinkii and a novel hydrogenotrophic methanogen were found in higher relative abundance and higher gene copy numbers in flocculating communities than in planktonic communities in the cultures, indicating benefits to syntrophs of living in close proximity to their cooperating partner. Microscopy and element analysis showed precipitation of phosphates and biofilm formation in all batches except on the graphene batches, possibly enhancing the rate of acetate and propionate degradation. Overall, the concordance of responses observed in both acetate- and propionate-fed cultures highlight the suitability of the addition of iron oxide or zeolites to enhance acid conversion to methane in high-ammonia biogas processes. KEY POINTS: • All additives promoted acetate (except graphene) and propionate degradation. • A preference for floc formation by ammonia-tolerant syntrophs was revealed. • Microbes colonised the surfaces of iron oxide and zeolite, but not graphene.
(© 2024. The Author(s).)

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948138 H2020 European Research Council; 2019-03846 Vetenskapsrådet

Keywords: Biogas; Graphene; Iron oxide; Syntrophy; Zeolite

0 (Propionates)
7664-41-7 (Ammonia)
0 (Acetates)
OP0UW79H66 (Methane)
1318-02-1 (Zeolites)
0 (Ferric Compounds)
7782-42-5 (Graphite)
1K09F3G675 (ferric oxide)
0 (Biofuels)

Date Created: 20240807 Date Completed: 20240807 Latest Revision: 20240810

20250114

PMC11306274

10.1007/s00253-024-13263-7

39110235