Comparison of the therapeutic effect of platelet-rich plasma and injectable platelet-rich fibrin on testicular torsion/detorsion injury in rats.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Platelet-Rich Plasma* ; Spermatic Cord Torsion*/therapy ; Rats, Wistar* ; Testis*/injuries ; Testis*/pathology ; Platelet-Rich Fibrin*/metabolism; Animals ; Male ; Rats ; Semen Analysis ; Tumor Necrosis Factor-alpha/metabolism ; Tumor Necrosis Factor-alpha/blood ; Interleukin-1beta/metabolism ; Interleukin-1beta/blood ; Disease Models, Animal ; Glutathione Peroxidase/metabolism

Testicular torsion is a common disorder in males and results in blockage of testicular circulation with subsequent damage of testicular germ cells. The current work aimed to compare the therapeutic effect of platelet-rich plasma (PRP) and injectable platelet-rich fibrin (i-PRF) on torsion/detorsion (T/D) injury in rats. Forty mature male Wister rats were arranged into 4 groups; (1) Control, (2) T/D, (3) T/D + PRP, and (4) T/D+ i-PRF. The right testis was twisting 1080° clockwise for 3 h in groups 2, 3 and 4, then 10 μl of PRP or i-PRF was injected intra-testicular 3 h after detorsion in groups 3 and 4, respectively. After 30 days postoperatively, the semen quality and hormonal assay were improved in PRP and i-PRF-treated groups with superiority of i-PRF (P < 0.001). High significance of Catalase, Glutathione Peroxidase (GPx), Superoxide Dismutase, Interleukin-1β (IL-1β), Caspase-3 and Tumor necrosis factor-α (TNF-α) was reported in treated rats with PRP and i-PRF (P < 0.001) with superiority to i-PRF-treated rats (P < 0.001). Testicular histoarchitectures were improved in PRP and i-PRF-treated rats with superiority of i-PRF-treated rats. It was concluded that PRP and i-PRF have regenerative efficacy on testicular damage after induced T/D injury with a superior efficacy of i-PRF.
(© 2024. The Author(s).)

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Keywords: Oxidative stress; PRP; Semen; Testicular torsion; Torsion/detorsion; i-PRF

0 (Tumor Necrosis Factor-alpha)
0 (Interleukin-1beta)
EC 1.11.1.9 (Glutathione Peroxidase)

Date Created: 20240805 Date Completed: 20240805 Latest Revision: 20240809

20240809

PMC11300838

10.1038/s41598-024-67704-4

39103420