Development and evaluation of a real-time multienzyme isothermal rapid amplification assay for rapid detection of Streptococcus pneumoniae.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Streptococcus pneumoniae*/genetics ; Streptococcus pneumoniae*/isolation & purification ; Pneumococcal Infections*/diagnosis ; Pneumococcal Infections*/microbiology ; Nucleic Acid Amplification Techniques*/methods ; Sensitivity and Specificity*; Humans ; Molecular Diagnostic Techniques/methods

Streptococcus pneumoniae is a significant pathogen causing infectious diseases, including pneumonia, otitis media, septicemia, and meningitis. The introduction of multivalent vaccines has coincided with a remarkable decrease in the number of pneumococcal-related deaths. Despite this, pneumococcal infection remains a significant cause of death among children under 5 years old and adults aged 65 or older at a global level. Therefore, early detection of S. pneumoniae infection is crucial for prognosis of pneumococcal infection patients. In this study, we evaluated the utility of a real-time multienzyme isothermal rapid amplification (MIRA) assay for detecting S. pneumoniae and other non-S. pneumoniae bacterial species. A primer-probe set targeting the S. pneumoniae lytA gene was designed, followed by optimization of parameters for the MIRA assay. At the same time, we validated the real-time MIRA assay for detecting S. pneumoniae using 79 clinical isolates identified by VITEK MS. The results showed a detection sensitivity and specificity of 100%. These results demonstrate that the designed real-time MIRA assay is a promising, rapid, simple, and reliable method for detecting S. pneumoniae infection in resource-limited areas. It has great potential for application in detecting not only S. pneumoniae but also other non-S. pneumoniae bacterial species.
(© 2024. The Author(s).)

Infect Immun. 2000 Mar;68(3):1374-82. (PMID: 10678950)
Nat Rev Microbiol. 2008 Apr;6(4):288-301. (PMID: 18340341)
J Invertebr Pathol. 2023 Nov;201:108017. (PMID: 37926345)
J Clin Microbiol. 2017 Dec 26;56(1):. (PMID: 29118170)
Vaccine. 2017 Apr 4;35(15):1817-1827. (PMID: 28284682)
Front Microbiol. 2022 Oct 13;13:1027785. (PMID: 36312945)
J Clin Microbiol. 2005 Sep;43(9):4528-34. (PMID: 16145102)
Front Microbiol. 2022 Jan 17;12:816997. (PMID: 35111144)
Vaccine. 2011 Sep 14;29 Suppl 3:C43-8. (PMID: 21896352)
Front Cell Infect Microbiol. 2022 Jan 18;11:804737. (PMID: 35118011)
J Med Microbiol. 2015 Aug;64(8):836-844. (PMID: 26066632)
Clin Lab. 2021 Nov 1;67(11):. (PMID: 34758242)
J Clin Microbiol. 2006 Apr;44(4):1250-6. (PMID: 16597847)
Clin Infect Dis. 2017 Jun 15;64(suppl_3):S347-S356. (PMID: 28575371)
J Infect Dis. 1996 Jan;173(1):113-8. (PMID: 8537647)
J Infect. 2010 Jul;61(2):114-24. (PMID: 20433866)
J Clin Microbiol. 2003 Sep;41(9):4415-7. (PMID: 12958280)
3 Biotech. 2023 Jun;13(6):200. (PMID: 37215369)
Front Cell Infect Microbiol. 2022 Oct 19;12:1010201. (PMID: 36339332)
J Clin Microbiol. 2003 Jan;41(1):63-6. (PMID: 12517826)
Lancet. 2009 Sep 12;374(9693):893-902. (PMID: 19748398)
Microorganisms. 2022 Aug 27;10(9):. (PMID: 36144336)
J Clin Microbiol. 2005 Apr;43(4):1581-6. (PMID: 15814969)
Adv Pharm Bull. 2022 Jan;12(1):58-76. (PMID: 35517891)
J Microbiol Methods. 2018 Jan;144:99-106. (PMID: 29162393)
Eur J Clin Microbiol Infect Dis. 2020 Dec;39(12):2247-2256. (PMID: 32710352)
J Clin Microbiol. 2009 Jun;47(6):1750-6. (PMID: 19386843)
Front Vet Sci. 2023 Jan 04;9:1059934. (PMID: 36686176)
Sci Rep. 2019 Mar 1;9(1):3285. (PMID: 30824850)
FEMS Microbiol Lett. 2010 Sep 1;310(1):48-53. (PMID: 20633048)
Diagn Microbiol Infect Dis. 2016 Jun;85(2):141-8. (PMID: 27107535)

LAMP assay

Date Created: 20240731 Date Completed: 20240801 Latest Revision: 20240808

20250114

PMC11291690

10.1038/s41598-024-68524-2

39085471