Isolation, Selection, and Identification of Keratinolytic Bacteria for Green Management of Keratin Waste.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Keratins*/metabolism ; Phylogeny* ; Biodegradation, Environmental* ; Bacteria*/metabolism ; Bacteria*/genetics; RNA, Ribosomal, 16S/genetics ; Soil Microbiology ; Animals ; Peptide Hydrolases/metabolism

The volume of difficult-to-process keratin waste is increasing as a result of rising global meat production. If not properly managed, this waste can contribute to environmental pollution and pose a threat to human and animal welfare. An interesting and more sustainable alternative is therefore the bioconversion of keratin using microorganisms and their enzymes. This work aimed to isolate bacteria from soil samples and zoonotic keratins and to evaluate their enzymatic capacity to degrade α- and β-keratin wastes. A total of 113 bacterial strains were isolated from environmental samples and subjected to taxonomic identification using the MALDI-TOF MS technique and to a two-step screening for proteolytic and keratinolytic activity. The ability to degrade a β-rich keratin substrate was observed in almost all of the strains isolated from soil and horsehairs. In contrast, when an α-rich keratin substrate was used, the highest levels of hydrolysis were observed only for Ker39, Ker66, Ker85, Ker100, and Ker101. Strains with the highest biodegradation potential were identified using molecular biology methods. Phylogenetic analysis of 16S rDNA gene sequences allowed the assignment of selected keratinolytic microorganisms to the genera Exiguobacterium , Priestia , Curtobacterium , Stenotrophomonas , Bacillus , Kocuria, or Pseudomonas . The results of this study are a promising precursor for the development of new, more sustainable methods of managing keratin waste to produce high-value hydrolysates.

G3 (Bethesda). 2020 Mar 5;10(3):881-890. (PMID: 31919110)
AMB Express. 2018 Jan 24;8(1):9. (PMID: 29368054)
Front Chem. 2021 Jul 27;9:715890. (PMID: 34386482)
Polymers (Basel). 2021 Jun 07;13(11):. (PMID: 34200460)
J Genet Eng Biotechnol. 2018 Dec;16(2):311-318. (PMID: 30733740)
Vet Res Commun. 2024 Jun 20;:. (PMID: 38900396)
Nat Commun. 2022 Sep 3;13(1):5195. (PMID: 36057639)
Microb Biotechnol. 2020 Mar;13(2):442-457. (PMID: 31613061)
Int J Mol Sci. 2021 Mar 18;22(6):. (PMID: 33803907)
Front Physiol. 2019 Apr 17;10:438. (PMID: 31057424)
Microorganisms. 2020 Jan 17;8(1):. (PMID: 31963469)
Microorganisms. 2023 Apr 24;11(5):. (PMID: 37317082)
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110612. (PMID: 32204061)
Int J Syst Evol Microbiol. 2016 Feb;66(2):1027-1033. (PMID: 26647141)
Biodegradation. 2011 Nov;22(6):1191-201. (PMID: 21526391)
Biomolecules. 2021 Dec 18;11(12):. (PMID: 34944542)
Vet Dermatol. 2021 Oct;32(5):467-e128. (PMID: 34165828)
Chemosphere. 2021 Dec;285:131466. (PMID: 34271468)
Microbiol Spectr. 2019 May;7(3):. (PMID: 31111815)
Food Technol Biotechnol. 2018 Sep;56(3):312-328. (PMID: 30510475)
Front Microbiol. 2016 Apr 21;7:542. (PMID: 27148228)
Front Microbiol. 2017 Mar 28;8:494. (PMID: 28400755)
Front Microbiol. 2018 Oct 15;9:2442. (PMID: 30374340)
Biotechnol Adv. 2020 Dec;45:107655. (PMID: 33186607)
Int J Biol Macromol. 2020 Nov 15;163:135-146. (PMID: 32615225)
Int J Syst Evol Microbiol. 2017 Aug;67(8):2499-2508. (PMID: 28792367)
Front Microbiol. 2016 Feb 12;7:104. (PMID: 26903983)
Sci Rep. 2017 Jul 5;7(1):4658. (PMID: 28680127)
FEMS Microbiol Ecol. 2022 Feb 21;98(1):. (PMID: 35142841)
Int J Biol Macromol. 2021 Jan 1;166:1046-1056. (PMID: 33157140)
Front Microbiol. 2018 May 18;9:780. (PMID: 29867782)
Extremophiles. 2020 Sep;24(5):693-704. (PMID: 32617734)
Mar Drugs. 2020 Oct 28;18(11):. (PMID: 33126528)
Brief Bioinform. 2019 Jul 19;20(4):1160-1166. (PMID: 28968734)
Biotechnol Lett. 2020 Nov;42(11):2403-2412. (PMID: 32642979)
PLoS One. 2018 Nov 26;13(11):e0206989. (PMID: 30475922)
Clin Microbiol Infect. 2021 Jan;27(1):126.e1-126.e5. (PMID: 32283265)
AIMS Microbiol. 2021 Dec 23;7(4):528-545. (PMID: 35071947)
Biotechnol Rep (Amst). 2020 May 29;27:e00483. (PMID: 32514407)
Arch Microbiol. 2020 Oct;202(8):2329-2336. (PMID: 32529508)
BMC Biotechnol. 2022 Sep 8;22(1):26. (PMID: 36076195)
J Genet Eng Biotechnol. 2022 May 25;20(1):81. (PMID: 35612674)
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2020;55(11):1339-1348. (PMID: 32664813)
Int J Mol Sci. 2019 Feb 04;20(3):. (PMID: 30720762)
Int J Mol Sci. 2022 Jan 21;23(3):. (PMID: 35163108)
Microorganisms. 2023 Feb 11;11(2):. (PMID: 36838421)

Keywords: isolation; keratin biodegradation; keratinases; keratinolytic bacteria; molecular identification

68238-35-7 (Keratins)
0 (RNA, Ribosomal, 16S)
EC 3.4.- (Peptide Hydrolases)

Date Created: 20240727 Date Completed: 20240728 Latest Revision: 20240904

20240904

PMC11280386

10.3390/molecules29143380

39064958