Pea Albumin Extracted from Pea ( Pisum sativum L.) Seeds Ameliorates High-Fat-Diet-Induced Non-Alcoholic Fatty Liver Disease by Regulating Lipogenesis and Lipolysis Pathways.

Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI Publishing

Non-alcoholic Fatty Liver Disease*/etiology ; Non-alcoholic Fatty Liver Disease*/prevention & control ; Non-alcoholic Fatty Liver Disease*/metabolism ; Lipogenesis*/drug effects ; Diet, High-Fat*/adverse effects ; Pisum sativum*/chemistry ; Lipolysis*/drug effects ; Seeds*/chemistry ; Mice, Inbred C57BL* ; Liver*/metabolism ; Liver*/drug effects; Animals ; Male ; Mice ; Insulin Resistance ; Disease Models, Animal ; Albumins/metabolism ; Pea Proteins/pharmacology ; Oxidative Stress/drug effects

Non-alcoholic fatty liver disease (NAFLD) is now recognized as the most prevalent liver disease globally. Pea albumin (PA) has demonstrated positive impacts on reducing obesity and improving glucose metabolism. In this research, a mouse model of NAFLD induced by a high-fat diet (HFD) was employed to examine the impact of PA on NAFLD and explore its potential mechanisms. The findings revealed that mice subjected to a HFD developed pronounced fatty liver alterations. The intervention with PA significantly lowered serum TC by 26.81%, TG by 43.55%, and LDL-C by 57.79%. It also elevated HDL-C levels by 1.2 fold and reduced serum ALT by 37.94% and AST by 31.21% in mice fed a HFD. These changes contributed to the reduction in hepatic steatosis and lipid accumulation. Additionally, PA improved insulin resistance and inhibited hepatic oxidative stress and inflammatory responses. Mechanistic studies revealed that PA alleviated lipid accumulation in HFD-induced NAFLD by activating the phosphorylation of AMPKα and ACC, inhibiting the expression of SREBF1 and FASN to reduce hepatic lipogenesis, and increasing the expression of ATGL, PPARα, and PPARγ to promote lipolysis and fatty acid oxidation. These results indicate that PA could serve as a dietary supplement for alleviating NAFLD, offering a theoretical foundation for the rational intake of PA in NAFLD intervention.
Competing Interests: F.W. was employed by Tibet Tianhong Science and Technology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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B18053 The 111 project from the Education Ministry of China

Keywords: hepatic steatosis; high-fat diet; lipogenesis; lipolysis; non-alcoholic fatty liver disease; pea albumin

0 (Albumins)
0 (Pea Proteins)

Date Created: 20240727 Date Completed: 20240727 Latest Revision: 20240729

20240729

PMC11280122

10.3390/nu16142232

39064674