Primary Cell Culture as a Model System for Evolutionary Molecular Physiology.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Evolution, Molecular* ; Primary Cell Culture*; Animals ; Humans ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism ; Models, Biological ; Phylogeny ; Fibroblasts/cytology ; Fibroblasts/metabolism ; Fibroblasts/physiology

Primary cell culture is a powerful model system to address fundamental questions about organismal physiology at the cellular level, especially for species that are difficult, or impossible, to study under natural or semi-natural conditions. Due to their ease of use, primary fibroblast cultures are the dominant model system, but studies using both somatic and germ cells are also common. Using these models, genome evolution and phylogenetic relationships, the molecular and biochemical basis of differential longevities among species, and the physiological consequences of life history evolution have been studied in depth. With the advent of new technologies such as gene editing and the generation of induced pluripotent stem cells (iPSC), the field of molecular evolutionary physiology will continue to expand using both descriptive and experimental approaches.

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Keywords: cell culture; comparative physiology; evolutionary physiology; fibroblasts

Date Created: 20240727 Date Completed: 20240727 Latest Revision: 20240729

20240729

PMC11277064

10.3390/ijms25147905

39063147