Osmotically Sensitive TREK Channels in Rat Articular Chondrocytes: Expression and Functional Role.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Chondrocytes*/metabolism ; Potassium Channels, Tandem Pore Domain*/metabolism ; Potassium Channels, Tandem Pore Domain*/genetics ; Cartilage, Articular*/metabolism ; Cartilage, Articular*/cytology; Animals ; Rats ; Cells, Cultured ; Male ; Mechanotransduction, Cellular ; Patch-Clamp Techniques

Articular chondrocytes are the primary cells responsible for maintaining the integrity and functionality of articular cartilage, which is essential for smooth joint movement. A key aspect of their role involves mechanosensitive ion channels, which allow chondrocytes to detect and respond to mechanical forces encountered during joint activity; nonetheless, the variety of mechanosensitive ion channels involved in this process has not been fully resolved so far. Because some members of the two-pore domain potassium (K2P) channel family have been described as mechanosensors in other cell types, in this study, we investigate whether articular chondrocytes express such channels. RT-PCR analysis reveals the presence of TREK-1 and TREK-2 channels in these cells. Subsequent protein expression assessments, including Western blotting and immunohistochemistry, confirm the presence of TREK-1 in articular cartilage samples. Furthermore, whole-cell patch clamp assays demonstrate that freshly isolated chondrocytes exhibit currents attributable to TREK-1 channels, as evidenced by activation by arachidonic acid (AA) and ml335 and further inhibition by spadin. Additionally, exposure to hypo-osmolar shock activates currents, which can be attributed to the presence of TREK-1 channels, as indicated by their inhibition with spadin. Therefore, these findings highlight the expression of TREK channels in rat articular chondrocytes and suggest their potential involvement in regulating the integrity of cartilage extracellular matrix.

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Keywords: articular cartilage; mechanosensitive channels; osmosensitive channels; osteoarthrosis; two-pore K channels

0 (Potassium Channels, Tandem Pore Domain)
0 (potassium channel protein TREK-1)

Date Created: 20240727 Date Completed: 20240728 Latest Revision: 20240731

20250114

PMC11277475

10.3390/ijms25147848

39063089