Infection of mice by the enteroaggregative E. coli strain 042 and two mutant derivatives overexpressing virulence factors: impact on disease markers, gut microbiota and concentration of SCFAs in feces.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Virulence Factors*/genetics ; Virulence Factors*/metabolism ; Escherichia coli Infections*/microbiology ; Escherichia coli Infections*/metabolism ; Feces*/microbiology ; Escherichia coli*/pathogenicity ; Escherichia coli*/genetics ; Escherichia coli*/metabolism ; Gastrointestinal Microbiome* ; Escherichia coli Proteins*/genetics ; Escherichia coli Proteins*/metabolism; Animals ; Mice ; Fatty Acids, Volatile/metabolism ; Mutation ; Virulence/genetics ; Female ; Disease Models, Animal ; Gene Expression Regulation, Bacterial ; Biomarkers ; Trans-Activators

Several pathogenic Escherichia coli strains cause diarrhea. Enteroaggregative E. coli (EAEC) strains are one of the diarrheagenic pathotypes. EAEC cells form a "stacked-brick" arrangement over the intestinal epithelial cells. EAEC isolates express, among other virulence determinants, the AggR transcriptional activator and the aggregative adherence fimbriae (AAF). Overexpression of the aggR gene results in increased expression of virulence factors such as the aff genes, as well as several genes involved in specific metabolic pathways such as fatty acid degradation (fad) and arginine degradation (ast). To support the hypothesis that induction of the expression of some of these pathways may play a role in EAEC virulence, in this study we used a murine infection model to evaluate the impact of the expression of these pathways on infection parameters. Mice infected with a mutant derivative of the EAEC strain 042, characterized by overexpression of the aggR gene, showed increased disease symptoms compared to those exhibited by mice infected with the wild type (wt) strain 042. Several of these symptoms were not increased when the infecting mutant, which overexpressed aggR, lacked the fad and ast pathways. Therefore, our results support the hypothesis that different metabolic pathways contribute to EAEC virulence.
(© 2024. The Author(s).)

Mol Biol Evol. 2013 Apr;30(4):772-80. (PMID: 23329690)
PLoS One. 2013 Apr 22;8(4):e61217. (PMID: 23630581)
Eur J Epidemiol. 2001;17(12):1125-30. (PMID: 12530772)
Nat Rev Microbiol. 2004 Feb;2(2):123-40. (PMID: 15040260)
J Infect Dis. 1989 Jun;159(6):1061-4. (PMID: 2656875)
Infect Immun. 2013 Apr;81(4):1164-71. (PMID: 23357383)
PLoS Negl Trop Dis. 2020 Sep 8;14(9):e0008613. (PMID: 32898134)
PLoS Pathog. 2010 Jun 17;6(6):e1000899. (PMID: 20585552)
FEMS Immunol Med Microbiol. 2012 Dec;66(3):281-98. (PMID: 22775224)
mBio. 2022 Jun 28;13(3):e0015722. (PMID: 35536001)
Microbiol Mol Biol Rev. 2019 Jun 5;83(3):. (PMID: 31167904)
Nucleic Acids Res. 2014 Jan;42(Database issue):D633-42. (PMID: 24288368)
Microbiol Spectr. 2021 Oct 31;9(2):e0136921. (PMID: 34612688)
Animals (Basel). 2022 Apr 08;12(8):. (PMID: 35454206)
Front Cell Infect Microbiol. 2020 Sep 18;10:485. (PMID: 33072619)
J Biol Chem. 2003 Nov 14;278(46):45680-9. (PMID: 12933818)
Infect Immun. 1985 May;48(2):378-83. (PMID: 2859247)
Euro Surveill. 2011 Jun 16;16(24):. (PMID: 21699770)
mSystems. 2024 Jun 18;9(6):e0134823. (PMID: 38742910)
Nutrients. 2017 Dec 11;9(12):. (PMID: 29232896)
Microbiology (Reading). 2009 Feb;155(Pt 2):521-530. (PMID: 19202100)
PLoS One. 2016 May 03;11(5):e0154823. (PMID: 27139220)
Anal Biochem. 2021 Jan 1;612:114016. (PMID: 33188741)
J Infect Dis. 1995 Feb;171(2):465-8. (PMID: 7844392)
Lancet Infect Dis. 2017 Sep;17(9):909-948. (PMID: 28579426)
J Trauma Acute Care Surg. 2022 Aug 1;93(2):137-146. (PMID: 35324554)
Infect Immun. 2013 Jan;81(1):122-32. (PMID: 23090962)
J Exp Med. 2019 Jan 7;216(1):84-98. (PMID: 30563917)
Nat Protoc. 2008;3(6):1101-8. (PMID: 18546601)
Clin Microbiol Rev. 1998 Jan;11(1):142-201. (PMID: 9457432)
Clin Infect Dis. 2001 Jun 15;32(12):1706-9. (PMID: 11360211)
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6. (PMID: 23193283)
Sci Rep. 2020 Feb 19;10(1):2926. (PMID: 32076042)
Lancet. 2013 Jul 20;382(9888):209-22. (PMID: 23680352)
Commun Biol. 2021 Nov 16;4(1):1295. (PMID: 34785760)
Genome Biol. 2011 Jun 24;12(6):R60. (PMID: 21702898)
J Clin Microbiol. 2000 Jan;38(1):7-12. (PMID: 10618054)
Microbiome. 2022 May 28;10(1):79. (PMID: 35643532)
Appl Environ Microbiol. 2005 Dec;71(12):8228-35. (PMID: 16332807)
PLoS One. 2013 Jul 30;8(7):e69971. (PMID: 23936127)
Front Immunol. 2023 Oct 18;14:1279837. (PMID: 37920459)
Microbes Infect. 2014 Nov;16(11):954-61. (PMID: 25267358)
PLoS One. 2010 Mar 10;5(3):e9490. (PMID: 20224823)
Nutrients. 2016 Oct 22;8(10):. (PMID: 27782068)
J Pediatr Gastroenterol Nutr. 1995 Aug;21(2):137-44. (PMID: 7472898)
Cell. 2016 Jun 2;165(6):1332-1345. (PMID: 27259147)
Infect Immun. 1997 Oct;65(10):4135-45. (PMID: 9317019)
Bioinformatics. 2011 Mar 15;27(6):863-4. (PMID: 21278185)
J Clin Invest. 2002 Nov;110(9):1329-37. (PMID: 12417572)
Sci Rep. 2017 Sep 8;7(1):10957. (PMID: 28887521)
Mol Microbiol. 2019 Feb;111(2):534-551. (PMID: 30485564)
Int J Mol Sci. 2020 Sep 15;21(18):. (PMID: 32942624)
Pediatr Infect Dis J. 1987 Sep;6(9):829-31. (PMID: 3313248)
Science. 2017 Aug 11;357(6351):570-575. (PMID: 28798125)
PLoS One. 2010 Jan 20;5(1):e8801. (PMID: 20098708)
Front Cell Infect Microbiol. 2022 May 30;12:885191. (PMID: 35706909)

JDC2022-050269-I Ministerio de Ciencia, Innovación y Universidades; CEX2021-001189-S/ MCIN/AEI / 10.13039/501100011033 Ministerio de Ciencia e Innovación; MICIN/AEI/FEDER (CEX2021-001234-M) Ministerio de Ciencia e Innovación; PID2019-107479RB-I00 Ministerio de Economía y Competitividad; 2021SGR300 Generalitat de Catalunya; CDEIGENT 2020-02 Generalitat Valenciana

Keywords: AggR; Enteroaggregative E. coli; Infection; Mice; Microbiota; SCFAs

0 (Virulence Factors)
0 (Escherichia coli Proteins)
0 (Fatty Acids, Volatile)
0 (AggR protein, E coli)
0 (Biomarkers)
0 (Trans-Activators)

Date Created: 20240723 Date Completed: 20240723 Latest Revision: 20240805

20240805

PMC11266498

10.1038/s41598-024-67731-1

39043759