Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency.

Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Boron*/deficiency ; Boron*/metabolism ; Nicotiana*/genetics ; Nicotiana*/metabolism ; Transcriptome* ; Proteome*/metabolism; Gene Expression Regulation, Plant ; Seedlings/genetics ; Seedlings/metabolism ; Seedlings/growth & development ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Gene Expression Profiling

Background: Boron (B) is an essential micronutrient for plants. Inappropriate B supply detrimentally affects the productivity of numerous crops. Understanding of the molecular responses of plants to different B supply levels would be of significance in crop improvement and cultivation practices to deal with the problem.
Results: We conducted a comprehensive analysis of the transcriptome and proteome of tobacco seedlings to investigate the expression changes of genes/proteins in response to different B supply levels, with a particular focus on B deficiency. The global gene and protein expression profiles revealed the potential mechanisms involved in the responses of tobacco to B deficiency, including up-regulation of the NIP5;1-BORs module, complex regulation of genes/proteins related to cell wall metabolism, and up-regulation of the antioxidant machinery.
Conclusion: Our results demonstrated that B deficiency caused severe morphological and physiological disorders in tobacco seedlings, and revealed dynamic expression changes of tobacco genes/proteins in response to different B supply levels, especially to B deficiency, thus offering valuable insights into the molecular responses of tobacco to B deficiency.
(© 2024. The Author(s).)

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Keywords: Antioxidant machinery; BOR; Boron; Cell wall-related genes; NIP; Proteome analysis; Tobacco; Transcriptome analysis

N9E3X5056Q (Boron)
0 (Proteome)
0 (Plant Proteins)

Date Created: 20240719 Date Completed: 20240719 Latest Revision: 20240725

20240726

PMC11264865

10.1186/s12870-024-05391-z

39030471