Escherichia coli Activate Extraintestinal Antibody Response and Provide Anti-Infective Immunity.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Escherichia coli*/immunology ; Antibodies, Bacterial*/immunology ; Antibodies, Bacterial*/blood ; Bacterial Outer Membrane Proteins*/immunology; Humans ; Adult ; Female ; Staphylococcus aureus/immunology ; Male ; Antibody Formation/immunology ; Middle Aged ; Escherichia coli Proteins/immunology ; Young Adult ; Aged ; Staphylococcal Infections/immunology ; Staphylococcal Infections/microbiology ; Adolescent ; Escherichia coli Infections/immunology ; Escherichia coli Infections/microbiology

The effects of intestinal microflora on extraintestinal immune response by intestinal cytokines and metabolites have been documented, but whether intestinal microbes stimulate serum antibody generation is unknown. Here, serum antibodies against 69 outer membrane proteins of Escherichia coli , a dominant bacterium in the human intestine, are detected in 141 healthy individuals of varying ages. Antibodies against E. coli outer membrane proteins are determined in all serum samples tested, and frequencies of antibodies to five outer membrane proteins (OmpA, OmpX, TsX, HlpA, and FepA) are close to 100%. Serum antibodies against E. coli outer membrane proteins are further validated by Western blot and bacterial pull-down. Moreover, the present study shows that OstA, HlpA, Tsx, NlpB, OmpC, YfcU, and OmpA provide specific immune protection against pathogenic E. coli , while HlpA and OmpA also exhibit cross-protection against Staphylococcus aureus infection. These finding indicate that intestinal E. coli activate extraintestinal antibody responses and provide anti-infective immunity.

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32270199 National Natural Science Foundation of China; 311020006 Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)

Keywords: Escherichia coli; acquired immunity; antibody; extraintestinal; intestinal microflora; outer membrane proteins

0 (Antibodies, Bacterial)
0 (Bacterial Outer Membrane Proteins)
0 (Escherichia coli Proteins)

Date Created: 20240713 Date Completed: 20240713 Latest Revision: 20240715

20240715

PMC11242715

10.3390/ijms25137450

39000557