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Modulation of Bronchial Epithelial Barrier Integrity by Low Molecular Weight Components from Birch Pollen.

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  • معلومة اضافية
    • المصدر:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • الموضوع:
      Betula* ; Pollen* ; Bronchi*/metabolism ; Bronchi*/cytology ; Bronchi*/drug effects ; Epithelial Cells*/metabolism ; Epithelial Cells*/drug effects ; Molecular Weight*; Humans ; Cell Line ; Allergens ; Cytokines/metabolism ; Respiratory Mucosa/metabolism ; Respiratory Mucosa/drug effects
    • نبذة مختصرة :
      Pollen, in addition to allergens, comprise low molecular weight components (LMC) smaller than 3 kDa. Emerging evidence indicates the relevance of LMC in allergic immune responses. However, the interaction of birch pollen (BP)-derived LMC and epithelial cells has not been extensively studied. We investigated epithelial barrier modifications induced by exposure to BP LMC, using the human bronchial epithelial cell line 16HBE14o-. Epithelial cell monolayers were apically exposed to the major BP allergen Bet v 1, aqueous BP extract or BP-derived LMC. Barrier integrity after the treatments was monitored by measuring transepithelial electrical resistance at regular intervals and by using the xCELLigence Real-Time Cell Analysis system. The polarized release of cytokines 24 h following treatment was measured using a multiplex immunoassay. Epithelial barrier integrity was significantly enhanced upon exposure to BP LMC. Moreover, BP LMC induced the repair of papain-mediated epithelial barrier damage. The apical release of CCL5 and TNF-α was significantly reduced after exposure to BP LMC, while the basolateral release of IL-6 significantly increased. In conclusion, the results of our study demonstrate that BP-derived LMC modify the physical and immunological properties of bronchial epithelial cells and thus regulate airway epithelial barrier responses.
    • References:
      Immunity. 2015 Jul 21;43(1):29-40. (PMID: 26200011)
      Sci Rep. 2018 Jul 26;8(1):11245. (PMID: 30050168)
      J Biol Chem. 2011 Sep 9;286(36):31263-71. (PMID: 21771795)
      Biochem J. 2014 Feb 1;457(3):379-90. (PMID: 24171862)
      Pediatr Allergy Immunol. 2023 Mar;34 Suppl 28:e13854. (PMID: 37186333)
      Tissue Barriers. 2015 Apr 03;3(1-2):e983777. (PMID: 25838986)
      J Allergy Clin Immunol. 2002 May;109(5):831-8. (PMID: 11994708)
      Tissue Barriers. 2015 Jul 15;3(3):e1062316. (PMID: 26451347)
      Clin Transl Allergy. 2020 Aug 31;10:36. (PMID: 32884636)
      Front Immunol. 2013 Nov 04;4:356. (PMID: 24204367)
      J Invest Dermatol. 2015 Jul;135(7):1790-1800. (PMID: 25705851)
      J Allergy Clin Immunol. 2004 Jun;113(6):1152-60. (PMID: 15208598)
      Plants (Basel). 2023 Jan 12;12(2):. (PMID: 36679068)
      Allergy. 2015 Aug;70(8):944-54. (PMID: 25939785)
      J Exp Med. 2005 Feb 21;201(4):627-36. (PMID: 15728240)
      Front Immunol. 2015 Apr 02;6:147. (PMID: 25883597)
      Clin Exp Allergy. 2016 Oct;46(10):1355-65. (PMID: 27061126)
      J Allergy Clin Immunol. 2014 Sep;134(3):521-9. (PMID: 24880633)
      Allergy. 2015 Nov;70(11):1450-60. (PMID: 26214762)
      Front Mol Biosci. 2022 Jun 16;9:900533. (PMID: 35782860)
      Front Immunol. 2019 May 07;10:974. (PMID: 31134071)
      Mol Immunol. 2019 Aug;112:140-150. (PMID: 31102986)
      J Allergy Clin Immunol. 2011 Feb;127(2):454-461.e1-9. (PMID: 21281872)
      Biomolecules. 2023 Jul 19;13(7):. (PMID: 37509186)
      J Allergy Clin Immunol. 2013 Sep;132(3):665-675.e8. (PMID: 23632299)
      J Allergy Clin Immunol. 2020 Jun;145(6):1510-1516. (PMID: 32224275)
      Biosci Rep. 2018 Nov 28;38(6):. (PMID: 30369485)
      J Allergy Clin Immunol. 2012 Nov;130(5):1087-1096.e10. (PMID: 22840853)
      Int J Biol Sci. 2013 Sep 23;9(9):966-79. (PMID: 24155670)
    • Grant Information:
      P06 and P09 This study was supported by Danube Allergy Research Cluster projects P06 and P09 funded by the Country of Lower Austria
    • Contributed Indexing:
      Keywords: birch pollen; bronchial epithelial cells; cytokines; epithelial barrier integrity; low molecular weight components; transepithelial electrical resistance; xCELLigence
    • الرقم المعرف:
      0 (Allergens)
      0 (Cytokines)
    • الموضوع:
      Date Created: 20240713 Date Completed: 20240713 Latest Revision: 20240715
    • الموضوع:
      20240715
    • الرقم المعرف:
      PMC11242533
    • الرقم المعرف:
      10.3390/ijms25137374
    • الرقم المعرف:
      39000481