Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Enterococcus*/isolation & purification ; Enterococcus*/drug effects ; Fishes*/microbiology ; Anti-Bacterial Agents*/pharmacology ; Food Microbiology*; Animals ; Poland ; Microbial Sensitivity Tests ; Drug Resistance, Bacterial ; Seafood/microbiology

Competing Interests: The authors have declared that no competing interests exist.
The growing concern over antibiotic resistance in foodborne pathogens necessitates comprehensive assessments of its prevalence and associated risks in various food products. The present study aimed to assess the occurrence of Enterococcus spp. in samples of fish purchased at various points of sale in the Tricity region. The selection of products (n = 74) was based on their availability and included both fish caught in the Baltic region and products imported from, Vietnam, China, Norway, and European Union (EU) countries. For bacterial isolation, samples were inoculated into selective broth, and the growth of enterococci was assessed based on turbidity. Positive cultures were confirmed by a change in color in bromocresol purple broth and were isolated on Slanetz-Bartley agar. Bacteria were present in all tested samples regardless of the degree of raw material processing as follows: frozen (F)- 55%, fresh/raw (FS)- 70.6%, thawed (DF)- 30%, smoked (S)- 50%, and the packaging methods, modified atmosphere packaging (MAP)- 34.4%, unit packaging (UP)- 75%, and sold in bulk (SB)- 76.9%, with an overall frequency of occurrence of 58.1%. The number of bacteria ranged from not detected to 4.28-log cfu/g, with the lowest mean values for thawed fish and those packed in MAP. Tests conducted on 24 strains isolated from samples showed their varied sensitivity to tetracyclines. Single cases of multidrug resistance of the tested strains were also observed. The conducted statistical analysis did not show statistically significant differences in the count of enterococci based on the origin, degree of processing, or packaging (p < 0.05). Moreover, differences in strain sensitivity to ampicillin were observed. Detected cases of resistance, especially to tetracycline, require careful monitoring and action to limit the health risks associated with resistant bacterial strains in food products.
(Copyright: © 2024 Kukułowicz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Microorganisms. 2021 Sep 07;9(9):. (PMID: 34576796)
Braz J Biol. 2021 Oct-Dec;81(4):954-961. (PMID: 33053131)
J Clin Microbiol. 2021 Nov 18;59(12):e0021321. (PMID: 34550809)
Foodborne Pathog Dis. 2012 Oct;9(10):939-44. (PMID: 22953740)
J Food Sci Technol. 2022 Jul;59(7):2884-2893. (PMID: 35734123)
J Food Prot. 2016 Jul;79(7):1240-6. (PMID: 27357045)
Southeast Asian J Trop Med Public Health. 2006 Jan;37(1):162-70. (PMID: 16771230)
J Food Sci. 2016 Nov;81(11):M2799-M2807. (PMID: 27780292)
Front Vet Sci. 2023 Apr 20;10:1155772. (PMID: 37152689)
Front Microbiol. 2019 Apr 18;10:728. (PMID: 31057497)
J Vet Res. 2021 Dec 20;65(4):449-455. (PMID: 35111998)
Microbiol Spectr. 2014 Nov;5(6):. (PMID: 29125098)
Antibiotics (Basel). 2023 Apr 04;12(4):. (PMID: 37107066)
PLoS One. 2021 Nov 15;16(11):e0259584. (PMID: 34780540)
Virulence. 2012 Aug 15;3(5):421-33. (PMID: 23076243)
Microorganisms. 2021 Oct 26;9(11):. (PMID: 34835352)
FEMS Microbiol Rev. 2002 Jun;26(2):163-71. (PMID: 12069881)
Food Microbiol. 2022 Apr;102:103900. (PMID: 34809932)
Antibiotics (Basel). 2023 Aug 28;12(9):. (PMID: 37760672)
PLoS One. 2013;8(4):e59491. (PMID: 23560050)
Int J Environ Res Public Health. 2021 May 26;18(11):. (PMID: 34073520)
Animals (Basel). 2023 May 26;13(11):. (PMID: 37889631)

0 (Anti-Bacterial Agents)

Date Created: 20240709 Date Completed: 20240709 Latest Revision: 20240711

20250114

PMC11232986

10.1371/journal.pone.0306826

38980872