A Sweet Potato MYB Transcription Factor IbMYB330 Enhances Tolerance to Drought and Salt Stress in Transgenic Tobacco.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE

Original Publication: Basel : MDPI

Ipomoea batatas*/genetics ; Ipomoea batatas*/metabolism ; Nicotiana*/genetics ; Nicotiana*/metabolism ; Plants, Genetically Modified*/genetics ; Transcription Factors*/genetics ; Transcription Factors*/metabolism ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Gene Expression Regulation, Plant* ; Droughts*; Salt Tolerance/genetics ; Stress, Physiological/genetics ; Salt Stress/genetics

MYB transcription factors (TFs) play vital roles in plant growth, development, and response to adversity. Although the MYB gene family has been studied in many plant species, there is still little known about the function of R2R3 MYB TFs in sweet potato in response to abiotic stresses. In this study, an R2R3 MYB gene, IbMYB330 was isolated from sweet potato ( Ipomoea batatas ). IbMYB330 was ectopically expressed in tobacco and the functional characterization was performed by overexpression in transgenic plants. The IbMYB330 protein has a 268 amino acid sequence and contains two highly conserved MYB domains. The molecular weight and isoelectric point of IbMYB330 are 29.24 kD and 9.12, respectively. The expression of IbMYB330 in sweet potato is tissue-specific, and levels in the root were significantly higher than that in the leaf and stem. It showed that the expression of IbMYB330 was strongly induced by PEG-6000, NaCl, and H 2 O 2 . Ectopic expression of IbMYB330 led to increased transcript levels of stress-related genes such as SOD , POD , APX , and P5CS . Moreover, compared to the wild-type (WT), transgenic tobacco overexpression of IbMYB330 enhanced the tolerance to drought and salt stress treatment as CAT activity, POD activity, proline content, and protein content in transgenic tobacco had increased, while MDA content had decreased. Taken together, our study demonstrated that IbMYB330 plays a role in enhancing the resistance of sweet potato to stresses. These findings lay the groundwork for future research on the R2R3-MYB genes of sweet potato and indicates that IbMYB330 may be a candidate gene for improving abiotic stress tolerance in crops.

Plant Physiol Biochem. 2021 Nov;168:167-176. (PMID: 34634642)
Plant Commun. 2022 Sep 12;3(5):100332. (PMID: 35643086)
Cell. 2016 Oct 6;167(2):313-324. (PMID: 27716505)
Plant J. 2021 Aug;107(3):740-759. (PMID: 33978999)
BMC Plant Biol. 2023 Jan 9;23(1):17. (PMID: 36617566)
Plant Cell. 2011 May;23(5):1971-84. (PMID: 21610183)
Front Plant Sci. 2022 Jun 02;13:893896. (PMID: 35720610)
Plant Cell Rep. 2008 Oct;27(10):1677-86. (PMID: 18679687)
Trends Plant Sci. 2019 Oct;24(10):934-946. (PMID: 31358471)
Genomics. 2021 May;113(3):1565-1578. (PMID: 33819564)
Plant Physiol Biochem. 2020 Oct;155:105-113. (PMID: 32745929)
Plant J. 2022 Oct;112(2):339-351. (PMID: 35984735)
Front Plant Sci. 2019 Aug 15;10:1025. (PMID: 31475022)
Curr Opin Plant Biol. 2001 Oct;4(5):447-56. (PMID: 11597504)
Plant Physiol Biochem. 2019 Feb;135:385-394. (PMID: 30616113)
Front Plant Sci. 2022 May 05;13:852709. (PMID: 35599896)
Plant Biotechnol J. 2023 Aug;21(8):1560-1576. (PMID: 37140026)
Front Plant Sci. 2015 Jul 22;6:552. (PMID: 26347750)
Front Plant Sci. 2021 Feb 01;11:618835. (PMID: 33597960)
Genes (Basel). 2022 Oct 17;13(10):. (PMID: 36292768)
Plant Physiol. 2008 Feb;146(2):623-35. (PMID: 18162593)
Annu Rev Plant Biol. 2020 Apr 29;71:403-433. (PMID: 32167791)
Plant Cell Environ. 2020 Aug;43(8):1925-1943. (PMID: 32406163)
Plant Cell Rep. 2021 Feb;40(2):405-419. (PMID: 33331953)
BMC Plant Biol. 2023 Apr 28;23(1):227. (PMID: 37118665)
Trends Plant Sci. 2010 Oct;15(10):573-81. (PMID: 20674465)
Plant Cell Physiol. 2020 Apr 1;61(4):748-760. (PMID: 31917443)
Annu Rev Plant Biol. 2018 Apr 29;69:209-236. (PMID: 29489394)
Mol Cells. 2011 May;31(5):447-54. (PMID: 21399993)
Plant Physiol Biochem. 2019 Apr;137:179-188. (PMID: 30798172)
Front Genet. 2021 Aug 26;12:714696. (PMID: 34512725)
Front Plant Sci. 2022 May 04;13:848891. (PMID: 35599895)
New Phytol. 2019 Sep;223(4):1918-1936. (PMID: 31091337)
Int J Mol Sci. 2021 May 26;22(11):. (PMID: 34073446)
J Genet Eng Biotechnol. 2023 Jun 30;21(1):74. (PMID: 37389653)
BMC Plant Biol. 2022 Apr 8;22(1):182. (PMID: 35395715)
J Microbiol Biotechnol. 2013 Dec;23(12):1737-46. (PMID: 24378636)
Plant Sci. 2021 Sep;310:110983. (PMID: 34315599)
Biochem Genet. 2021 Jun;59(3):678-696. (PMID: 33502632)
Front Plant Sci. 2022 Jan 17;12:819247. (PMID: 35111187)
PLoS One. 2012;7(12):e52030. (PMID: 23251677)
Genes (Basel). 2022 Aug 18;13(8):. (PMID: 36011387)
BMC Plant Biol. 2018 Dec 3;18(1):320. (PMID: 30509166)
Plant Physiol Biochem. 2013 Sep;70:252-60. (PMID: 23800660)
BMC Plant Biol. 2022 Apr 26;22(1):213. (PMID: 35468742)
Int J Mol Sci. 2022 May 24;23(11):. (PMID: 35682553)
Plant Physiol Biochem. 2021 Sep;166:777-788. (PMID: 34217134)
Plant Physiol. 2020 Jun;183(2):637-655. (PMID: 32291329)
J Med Food. 2014 Jul;17(7):733-41. (PMID: 24921903)
Plant Direct. 2018 Oct 30;2(10):e00092. (PMID: 31245692)
Plant Physiol. 2003 Feb;131(2):610-20. (PMID: 12586885)
Sci Rep. 2022 Feb 18;12(1):2825. (PMID: 35181714)
Nat Plants. 2017 Sep;3(9):696-703. (PMID: 28827752)
Int J Mol Sci. 2015 Jul 13;16(7):15811-51. (PMID: 26184177)
Physiol Plant. 2021 Dec;173(4):1369-1381. (PMID: 33619766)
J Exp Bot. 2014 Aug;65(15):4255-69. (PMID: 24821954)
Plant Cell Physiol. 2016 Aug;57(8):1657-77. (PMID: 27279646)
Int J Mol Sci. 2021 Jun 07;22(11):. (PMID: 34200125)
PLoS One. 2012;7(6):e37463. (PMID: 22719841)
Plant J. 2021 Mar;105(6):1566-1581. (PMID: 33314379)
Mol Plant. 2022 Jul 4;15(7):1227-1242. (PMID: 35684964)
Plant Cell. 2003 Jan;15(1):63-78. (PMID: 12509522)
Genomics. 2022 Mar;114(2):110275. (PMID: 35108591)
Nat Rev Genet. 2022 Feb;23(2):104-119. (PMID: 34561623)
Nat Rev Mol Cell Biol. 2022 Oct;23(10):663-679. (PMID: 35760900)
Hortic Res. 2022 Mar 23;9:uhac073. (PMID: 35712696)
Antioxidants (Basel). 2022 Jan 25;11(2):. (PMID: 35204108)
Plant Sci. 2020 Apr;293:110323. (PMID: 32081254)
Sci Rep. 2016 Aug 30;6:32006. (PMID: 27573926)
Curr Biol. 2005 Jul 12;15(13):1201-6. (PMID: 16005292)

2019YFD1001300; 2019YFD1001305 National Key R&D Program of China; HBZY2023B002, HBZY2023B002-4 Funding for Seed Industry High-quality Development of Hubei Province; 2020-620-000-001-007 Hubei Provincial Agricultural Technology Innovation Center Funding Project; 2019TSXK06 Hubei Academy of Agricultural Sciences Sweet Potato Specialty Discipline Project; 2023ZZCX001 Construction and Demonstration of a Supporting Storage and Breeding Technology System for "Two Harvests a Year" of Sweet Potatoes

Keywords: IbMYB330; drought and salt tolerance; gene overexpression; sweet potato; tobacco

0 (Transcription Factors)
0 (Plant Proteins)

Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240716

20240717

PMC11202548

10.3390/genes15060693

38927629