Integration of Proteomic and Metabolomic Data Reveals the Lipid Metabolism Disorder in the Liver of Rats Exposed to Simulated Microgravity.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, 2011-

Liver*/metabolism ; Proteomics*/methods ; Metabolomics*/methods ; Lipid Metabolism* ; Weightlessness Simulation*; Animals ; Rats ; Male ; Rats, Sprague-Dawley ; Tandem Mass Spectrometry ; Chromatography, Liquid ; Lipid Metabolism Disorders/metabolism

Long-term exposure to microgravity is considered to cause liver lipid accumulation, thereby increasing the risk of non-alcoholic fatty liver disease (NAFLD) among astronauts. However, the reasons for this persistence of symptoms remain insufficiently investigated. In this study, we used tandem mass tag (TMT)-based quantitative proteomics techniques, as well as non-targeted metabolomics techniques based on liquid chromatography-tandem mass spectrometry (LC-MS/MS), to comprehensively analyse the relative expression levels of proteins and the abundance of metabolites associated with lipid accumulation in rat liver tissues under simulated microgravity conditions. The differential analysis revealed 63 proteins and 150 metabolites between the simulated microgravity group and the control group. By integrating differentially expressed proteins and metabolites and performing pathway enrichment analysis, we revealed the dysregulation of major metabolic pathways under simulated microgravity conditions, including the biosynthesis of unsaturated fatty acids, linoleic acid metabolism, steroid hormone biosynthesis and butanoate metabolism, indicating disrupted liver metabolism in rats due to weightlessness. Finally, we examined differentially expressed proteins associated with lipid metabolism in the liver of rats exposed to stimulated microgravity. These findings contribute to identifying the key molecules affected by microgravity and could guide the design of rational nutritional or pharmacological countermeasures for astronauts.

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No. 2021JZ-27 and No. 2017SF-187 Natural Science Foundation of Shaanxi Province; No. CBSKL2019-ZZ13 Foundation of Cancer Biology State Key Laboratory; No. 2020PT-019 Innovation Capability Support Program of Shaanxi Province

Keywords: lipid metabolism; metabolomics; proteomics; simulated microgravity

Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240629

20240629

PMC11201887

10.3390/biom14060682

38927087