Ischemic Post-Conditioning in a Rat Model of Asphyxial Cardiac Arrest.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI

Heart Arrest*/therapy ; Heart Arrest*/complications ; Heart Arrest*/physiopathology ; Rats, Wistar* ; Ischemic Postconditioning*/methods ; Disease Models, Animal* ; Asphyxia*/complications; Animals ; Male ; Rats ; Cardiopulmonary Resuscitation/methods ; Epinephrine

Background: Ischemic post-conditioning (IPoC) has been shown to improve outcomes in limited pre-clinical models. As down-time is often unknown, this technique needs to be investigated over a range of scenarios. As this tool limits reperfusion injury, there may be limited benefit or even harm after short arrest and limited ischemia-reperfusion injury.
Methods: Eighteen male Wistar rats underwent 7 min of asphyxial arrest. Animals randomized to IPoC received a 20 s pause followed by 20 s of compressions, repeated four times, initiated 40 s into cardiopulmonary resuscitation. If return of spontaneous circulation (ROSC) was achieved, epinephrine was titrated to mean arterial pressure (MAP) of 70 mmHg. Data were analyzed using t -test or Mann-Whitney test. Significance set at p ≤ 0.05.
Results: The rate of ROSC was equivalent in both groups, 88%. There was no statistically significant difference in time to ROSC, epinephrine required post ROSC, carotid flow, or peak lactate at any timepoint. There was a significantly elevated MAP with IPoC, 90.7 mmHg (SD 13.9), as compared to standard CPR, 76.7 mmHg (8.5), 2 h after ROSC, p = 0.03.
Conclusions: IPoC demonstrated no harm in a model of short arrest using a new arrest etiology for CPR based IPoC intervention in a rat model.

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5T32 GM108554 United States NH NIH HHS; I01 BX003482 United States BX BLRD VA; 4-04-300-5652 Society of Cardiovascular Anesthesiologists; 1187757 Foundation of Anesthesia Education and Research; 962204 American Heart Association; VDOA-CLIG-20221024 Vanderbilt University Department of Anesthesiology Crawford Long Innovation Grant; I01 BX005993 United States BX BLRD VA

Keywords: advanced cardiac life support; asphyxia; basic life support; cardiopulmonary resuscitation; compressions; ischemia-reperfusion injury; murine; resuscitation

YKH834O4BH (Epinephrine)

Date Created: 20240626 Date Completed: 20240626 Latest Revision: 20240808

20240808

PMC11201463

10.3390/cells13121047

38920675