Hippocampal-dependent navigation in head-fixed mice using a floating real-world environment.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Spatial Navigation*/physiology ; Maze Learning* ; Hippocampus*/physiology; Animals ; Mice ; Male ; Pyramidal Cells/physiology ; Mice, Inbred C57BL ; Membrane Potentials/physiology ; CA1 Region, Hippocampal/physiology ; Virtual Reality ; Scopolamine/pharmacology ; Patch-Clamp Techniques/methods

Head-fixation of mice enables high-resolution monitoring of neuronal activity coupled with precise control of environmental stimuli. Virtual reality can be used to emulate the visual experience of movement during head fixation, but a low inertia floating real-world environment (mobile homecage, MHC) has the potential to engage more sensory modalities and provide a richer experimental environment for complex behavioral tasks. However, it is not known whether mice react to this adapted environment in a similar manner to real environments, or whether the MHC can be used to implement validated, maze-based behavioral tasks. Here, we show that hippocampal place cell representations are intact in the MHC and that the system allows relatively long (20 min) whole-cell patch clamp recordings from dorsal CA1 pyramidal neurons, revealing sub-threshold membrane potential dynamics. Furthermore, mice learn the location of a liquid reward within an adapted T-maze guided by 2-dimensional spatial navigation cues and relearn the location when spatial contingencies are reversed. Bilateral infusions of scopolamine show that this learning is hippocampus-dependent and requires intact cholinergic signalling. Therefore, we characterize the MHC system as an experimental tool to study sub-threshold membrane potential dynamics that underpin complex navigation behaviors.
(© 2024. The Author(s).)

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101029/Z/13/Z United Kingdom WT_ Wellcome Trust; 202810/Z/16/Z United Kingdom WT_ Wellcome Trust

DL48G20X8X (Scopolamine)

Date Created: 20240621 Date Completed: 20240621 Latest Revision: 20240624

20240624

PMC11192748

10.1038/s41598-024-64807-w

38906952