A Lombard Variety of Sweet Pepper Regulating Senescence and Proliferation: The Voghera Pepper.

Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI Publishing

Fibroblasts*/drug effects ; Fibroblasts*/metabolism ; Capsicum*/chemistry ; Cellular Senescence*/drug effects ; Plant Extracts*/pharmacology ; Cell Proliferation*/drug effects ; Ascorbic Acid*/pharmacology ; Tumor Suppressor Protein p53*/metabolism; Humans ; Proliferating Cell Nuclear Antigen/metabolism ; Cyclin-Dependent Kinase Inhibitor p16/metabolism ; Aging/physiology ; Antioxidants/pharmacology ; Diploidy ; Cells, Cultured ; Italy

Aging and its related disorders are important issues nowadays and the first cause of this physio-pathological condition is the overproduction of ROS. Ascorbic acid is an antioxidant mediator and its anti-aging proprieties are well known. Our previous data demonstrated that Voghera sweet pepper (VP), a distinctive type of pepper cultivated in Italy, is particularly rich in ascorbic acid. Based on these data, the anti-aging effect mediated by extracts of the edible part of VP was evaluated on an in vitro model of both young and old Normal Human Diploid Fibroblasts (NHDF). Using phase contrast microscopy, we observed that VP may help cells in the maintenance of physiological morphology during aging. Cytofluorimetric analyses revealed that VP extracts led to an increase in DNA synthesis and percentage of living cells, linked to a consequent increase in mitotic events. This hypothesis is supported by the enhancement of PCNA expression levels observed in old, treated fibroblasts, corroborating the idea that this extract could recover a young phenotype in adult fibroblasts, confirmed by the study of p16 and p53 expression levels and TEM analyses. Based on these results, we may suppose that VP can lead to the partial recovery of "young-like" phenotypes in old fibroblasts.

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ECS00000036 NODES, MUR - M4C2 1.5, PNRR, European Union - NextGenerationEU

Keywords: Voghera sweet pepper; aging; ascorbic acid; cell proliferation; normal human dermal fibroblast; nutraceuticals; phytotherapy; senescence

0 (Plant Extracts)
PQ6CK8PD0R (Ascorbic Acid)
0 (Tumor Suppressor Protein p53)
0 (Proliferating Cell Nuclear Antigen)
0 (Cyclin-Dependent Kinase Inhibitor p16)
0 (Antioxidants)
0 (CDKN2A protein, human)
0 (TP53 protein, human)

Date Created: 20240619 Date Completed: 20240619 Latest Revision: 20240620

20240620

PMC11174795

10.3390/nu16111681

38892614