High expression levels of centromere protein O participates in cell proliferation of human ovarian cancer.

Publisher: BioMed Central Country of Publication: England NLM ID: 101474849 Publication Model: Electronic Cited Medium: Internet ISSN: 1757-2215 (Electronic) Linking ISSN: 17572215 NLM ISO Abbreviation: J Ovarian Res Subsets: MEDLINE

Original Publication: [London] : BioMed Central, c2008-

Apoptosis*/genetics ; Cell Proliferation*/genetics ; Chromosomal Proteins, Non-Histone*/genetics ; Chromosomal Proteins, Non-Histone*/metabolism ; Ovarian Neoplasms*/genetics ; Ovarian Neoplasms*/metabolism ; Ovarian Neoplasms*/pathology; Female ; Humans ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic

Ovarian cancer is a common malignant tumor in women, with a high mortality rate ranking first among gynecological tumors. Currently, there is insufficient understanding of the causes, pathogenesis, recurrence and metastasis of ovarian cancer, and early diagnosis and treatment still face great challenges. The sensitivity and specificity of existing ovarian cancer screening methods are still unsatisfactory. Centromere protein O (CENP-O) is a recently discovered structural centromere protein that is involved in cell death and is essential for spindle assembly, chromosome separation, and checkpoint signaling during mitosis. The abnormal high expression of CENP-O was detected in various tumors such as bladder cancer and gastric cancer, and it participates in the regulation of tumor cell proliferation. In this study, we detect the expression abundance of CENP-O mRNA in different ovarian cancer cells ( ES-2, A2780, Caov-3, OVCAR-3 and SK-OV-3). The biological function changes of cell proliferation and apoptosis were detected and the role of CENP-O in ovarian cancer cell proliferation and apoptosis was explored by knocking down the expression of CENP-O gene. The results showed that CENP-O gene was significantly expressed in 5 types of ovarian cancer cell lines. After knocking down the CENP-O gene, the proliferation and cloning ability of ovarian cancer cells decreased, and the apoptosis increased. This study indicates that CENP-O has the potential to be a molecular therapeutic target, and downregulating the expression of CENP-O gene can break the unlimited proliferation ability of cancer cells and promote their apoptosis, providing a foundation and new ideas for subsequent molecular mechanism research and targeted therapy.
(© 2024. The Author(s).)

Nucleic Acids Res. 2019 Feb 20;47(3):1051-1069. (PMID: 30590707)
Genes Cells. 2004 Feb;9(2):105-20. (PMID: 15009096)
Life Sci. 2018 Sep 1;208:192-200. (PMID: 30031812)
Int J Gen Med. 2022 Sep 25;15:7493-7510. (PMID: 36187159)
Gynecol Oncol. 2020 Apr;157(1):222-233. (PMID: 31987601)
Dis Esophagus. 2013 Aug;26(6):636-43. (PMID: 23163484)
Epigenetics Chromatin. 2015 Jan 13;8:2. (PMID: 25788983)
Chromosoma. 2011 Oct;120(5):425-46. (PMID: 21751032)
Cell Mol Biol Lett. 2018 Jan 03;23:2. (PMID: 29308068)
Oncol Rep. 2013 Nov;30(5):2238-44. (PMID: 23970101)
Mol Med Rep. 2019 Oct;20(4):3661-3670. (PMID: 31485675)
PeerJ. 2015 Nov 05;3:e1386. (PMID: 26587348)
Int J Gynecol Cancer. 2017 Nov;27(9S Suppl 5):S20-S22. (PMID: 29278600)
Ann Oncol. 2020 Sep;31(9):1148-1159. (PMID: 32569725)
J Rheumatol. 2009 Apr;36(4):781-6. (PMID: 19286853)
Cell Chem Biol. 2020 Jul 16;27(7):850-857.e6. (PMID: 32442423)
J Cell Sci. 2015 Jan 1;128(1):171-84. (PMID: 25395579)
Gynecol Oncol. 2015 Apr;137(1):173-9. (PMID: 25677064)
Cancer Res. 2005 Jun 15;65(12):5181-9. (PMID: 15958562)
Curr Opin Cell Biol. 2012 Feb;24(1):64-70. (PMID: 22196931)
Nurse Pract. 2015 Sep 13;40(9):47-54. (PMID: 26274886)
Nature. 2016 Sep 8;537(7619):249-253. (PMID: 27580032)
Oncology (Williston Park). 2006 Sep;20(10):1156, 1158. (PMID: 17024868)
Nat Rev Genet. 2012 Jan 24;13(3):189-203. (PMID: 22269907)
BMC Cancer. 2023 Aug 9;23(1):735. (PMID: 37558987)
J Cancer. 2019 Jun 9;10(16):3728-3734. (PMID: 31333790)
Mol Cell Biol. 2005 Dec;25(24):10875-94. (PMID: 16314512)
Leukemia. 2002 May;16(5):767-75. (PMID: 11986936)
In Vitro Cell Dev Biol. 1988 Apr;24(4):266-73. (PMID: 3284875)
Clin Cancer Res. 2007 Jan 15;13(2 Pt 1):508-14. (PMID: 17255272)
CA Cancer J Clin. 2019 Jul;69(4):280-304. (PMID: 31099893)
CA Cancer J Clin. 2007 Jan-Feb;57(1):43-66. (PMID: 17237035)
Best Pract Res Clin Obstet Gynaecol. 2017 May;41:3-14. (PMID: 27743768)
Adv Exp Med Biol. 2010;676:93-103. (PMID: 20687471)

Keywords: Cell apoptosis; Cell cycle; Cell proliferation; Centromere protein O; Ovarian cancer

0 (Chromosomal Proteins, Non-Histone)
0 (CENPO protein, human)

Date Created: 20240618 Date Completed: 20240619 Latest Revision: 20240624

20240625

PMC11184697

10.1186/s13048-024-01452-x

38890751