Transcriptome analyses of Acer Truncatum Bunge seeds to delineate the genes involved in fatty acid metabolism.

Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE

Original Publication: London : BioMed Central, [2000-

Seeds*/genetics ; Seeds*/metabolism ; Seeds*/growth & development ; Acer*/genetics ; Acer*/metabolism ; Acer*/growth & development ; Fatty Acids*/metabolism ; Gene Expression Profiling* ; Gene Expression Regulation, Plant*; Transcriptome ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Genes, Plant ; Fatty Acids, Monounsaturated

Background: Acer truncatum Bunge is an economic, ecological, oil, and medicinal tree, and its kernel oil is rich in nervonic acid. It is crucial to explore the transcriptional expression patterns of genes affecting fatty acid synthesis to improve the quality of Acer truncatum oil.
Results: This study used the seeds from high fatty acid strain YQC and those from low fatty acid strain Y38 as the test materials. Specifically, we performed a comparative transcriptome analysis of Y38 seeds and YQC to identify differentially expressed genes (DEGs) at two time points (seeds 30 days after the blooming period and 90 days after the blooming period). Compared with YQC_1 (YQC seeds at 30 days after the blooming period), a total of 3,618 DEGs were identified, including 2,333 up-regulated and 1,285 downregulated DEGs in Y38_1 (Y38 seeds at 30 days after blooming period). In the Y38_2 (Y38 seeds at 90 days after the blooming period) versus YQC_2 (YQC seeds at 90 days after the blooming period) comparison group, 9,340 genes were differentially expressed, including 5,422 up-regulated and 3,918 down-regulated genes. The number of DEGs in Y38 compared to YQC was significantly higher in the late stages of seed development. Gene functional enrichment analyses showed that the DEGs were mainly involved in the fatty acid biosynthesis pathway. And two fatty acid synthesis-related genes and seven nervonic acid synthesis-related genes were validated by qRT-PCR.
Conclusions: This study provides a basis for further research on biosynthesizing fatty acids and nervonic acidnervonic acids in A. truncatum seeds.
(© 2024. The Author(s).)

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2020LZGC00903 the subject of key R & D plan of Shandong Province (Agricultural Elite Varieties Project)

Keywords: Acer Truncatum Bunge; Fatty acid; Nervonic acid; Transcriptome

0 (Fatty Acids)
0 (Plant Proteins)
91OQS788BE (nervonic acid)
0 (Fatty Acids, Monounsaturated)

Date Created: 20240617 Date Completed: 20240618 Latest Revision: 20240620

20240620

PMC11181630

10.1186/s12864-024-10481-1

38886635