Intraluminal oxygen can keep small bowel mucosa intact in a segmental ischemia model.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Intestinal Mucosa*/metabolism ; Intestinal Mucosa*/pathology ; Intestinal Mucosa*/blood supply ; Intestine, Small*/metabolism ; Intestine, Small*/blood supply ; Intestine, Small*/pathology ; Oxygen*/metabolism ; Ischemia*/metabolism ; Ischemia*/pathology ; Ischemia*/therapy; Animals ; Swine ; Disease Models, Animal ; Organ Preservation/methods ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism

Intestinal preservation for transplantation is accompanied by hypoperfusion with long periods of ischemia with total blood cessation and absolute withdrawal of oxygen leading to structural damage. The application of intraluminal oxygen has been successfully tested in small-animal series during storage and transport of the organ but have been so far clinically unrelatable. In this study, we tested whether a simple and clinically approachable method of intraluminal oxygen application could prevent ischemic damage in a large animal model, during warm ischemia time. We utilised a local no-flow ischemia model of the small intestine in pigs. A low-flow and high-pressure intraluminal oxygen deliverance system was applied in 6 pigs and 6 pigs served as a control group. Mucosal histopathology, hypoxia and barrier markers were evaluated after two hours of no-flow conditions, in both treatment and sham groups, and in healthy tissue. Macro- and microscopically, the luminal oxygen delivered treatment group showed preserved small bowel's appearance, viability, and mucosal integrity. A gradual deterioration of histopathology and barrier markers and increase in hypoxia-inducible factor 1-α expression towards the sites most distant from the oxygen application was observed. Intraluminal low-flow, high oxygen delivery can preserve the intestinal mucosa during total ischemia of the small intestine. This finding can be incorporated in methods to overcome small bowel ischemia and improve intestinal preservation for transplantation.
(© 2024. The Author(s).)

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Euregio-project 2-EUR-II-2=15 Interreg IIIA, European Union; Euregio-project 2-EUR-II-2=15 Interreg IIIA, European Union; Euregio-project 2-EUR-II-2=15 Interreg IIIA, European Union; Euregio-project 2-EUR-II-2=15 Interreg IIIA, European Union

Keywords: Animal disease models; Intestinal Mucosa; Intestinal transplantation; Ischemia; Oxygen; Preservation; Small intestine

S88TT14065 (Oxygen)
0 (Hypoxia-Inducible Factor 1, alpha Subunit)

Date Created: 20240614 Date Completed: 20240615 Latest Revision: 20240618

20240618

PMC11178904

10.1038/s41598-024-64660-x

38877069