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Microbially induced calcium carbonate precipitation through CO 2 sequestration via an engineered Bacillus subtilis.

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  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101139812 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2859 (Electronic) Linking ISSN: 14752859 NLM ISO Abbreviation: Microb Cell Fact Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central, [2002-
    • الموضوع:
      Calcium Carbonate*/metabolism ; Calcium Carbonate*/chemistry ; Bacillus subtilis*/metabolism ; Bacillus subtilis*/genetics ; Bacillus subtilis*/enzymology ; Carbon Dioxide*/metabolism ; Carbonic Anhydrases*/metabolism ; Carbonic Anhydrases*/genetics ; Sporosarcina*/metabolism ; Sporosarcina*/enzymology ; Sporosarcina*/genetics; Bacillus megaterium/metabolism ; Bacillus megaterium/genetics ; Bacillus megaterium/enzymology ; Carbon Sequestration ; Chemical Precipitation ; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics
    • نبذة مختصرة :
      Background: Microbially induced calcium carbonate precipitation has been extensively researched for geoengineering applications as well as diverse uses within the built environment. Bacteria play a crucial role in producing calcium carbonate minerals, via enzymes including carbonic anhydrase-an enzyme with the capability to hydrolyse CO 2 , commonly employed in carbon capture systems. This study describes previously uncharacterised carbonic anhydrase enzyme sequences capable of sequestering CO2 and subsequentially generating CaCO 3 biominerals and suggests a route to produce carbon negative cementitious materials for the construction industry.
      Results: Here, Bacillus subtilis was engineered to recombinantly express previously uncharacterised carbonic anhydrase enzymes from Bacillus megaterium and used as a whole cell catalyst allowing this novel bacterium to sequester CO 2 and convert it to calcium carbonate. A significant decrease in CO 2 was observed from 3800 PPM to 820 PPM upon induction of carbonic anhydrase and minerals recovered from these experiments were identified as calcite and vaterite using X-ray diffraction. Further experiments mixed the use of this enzyme (as a cell free extract) with Sporosarcina pasteurii to increase mineral production whilst maintaining a comparable level of CO 2 sequestration.
      Conclusion: Recombinantly produced carbonic anhydrase successfully sequestered CO 2 and converted it into calcium carbonate minerals using an engineered microbial system. Through this approach, a process to manufacture cementitious materials with carbon sequestration ability could be developed.
      (© 2024. The Author(s).)
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    • Grant Information:
      E3 2019 Research England; E3 2019 Research England; E3 2019 Research England; E3 2019 Research England; E3 2019 Research England; E3 2019 Research England; EP/R003777/1 Engineering and Physical Sciences Research Council,United Kingdom; EP/R003777/1 Engineering and Physical Sciences Research Council,United Kingdom; EP/R003629/1 Engineering and Physical Sciences Research Council
    • Contributed Indexing:
      Keywords: Calcite; Carbon capture; Carbon sequestration; Carbonic anhydrase; MICP; Recombinant protein expression
    • الرقم المعرف:
      H0G9379FGK (Calcium Carbonate)
      142M471B3J (Carbon Dioxide)
      EC 4.2.1.1 (Carbonic Anhydrases)
      0 (Bacterial Proteins)
    • الموضوع:
      Sporosarcina pasteurii
    • الموضوع:
      Date Created: 20240610 Date Completed: 20240611 Latest Revision: 20240613
    • الموضوع:
      20240613
    • الرقم المعرف:
      PMC11163794
    • الرقم المعرف:
      10.1186/s12934-024-02437-7
    • الرقم المعرف:
      38858761