Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
Ethanolic extract from Sophora moorcroftiana inhibit cell proliferation and alter the mechanical properties of human cervical cancer.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
- معلومة اضافية
- المصدر:
Publisher: BioMed Central Country of Publication: England NLM ID: 101761232 Publication Model: Electronic Cited Medium: Internet ISSN: 2662-7671 (Electronic) Linking ISSN: 26627671 NLM ISO Abbreviation: BMC Complement Med Ther Subsets: MEDLINE
- بيانات النشر:
Original Publication: London : BioMed Central, [2020]-
- الموضوع:
- نبذة مختصرة :
Background: Cervical cancer is one of the most common gynecological malignancies. Previous studies have shown that the ethanol extract of Sophora moorcroftiana seeds (EESMS) possesses an antiproliferative effect on several tumors in vitro. Therefore, in this study, we assessed the impact of EESMS on human cervical carcinoma (HeLa) cell proliferation.
Methods: The proliferation and apoptotic effects of HeLa cells treated with EESMS were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, dual acridine orange/ethidium bromide double staining, flow cytometry, and western blotting. Single-cell level atomic force microscopy (AFM) was conducted to detect the mechanical properties of HeLa cells, and proteomics and bioinformatics methods were used to elucidate the molecular mechanisms of EESMS.
Results: EESMS treatment inhibited HeLa cell proliferation by blocking the G0/G1 phase, increasing the expression of Caspase-3 and affecting its mechanical properties, and the EESMS indicated no significant inhibitory effect on mouse fibroblasts L929 cell line. In total, 218 differentially expressed proteins were identified using two-dimensional electrophoresis, and eight differentially expressed proteins were successfully identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. The differentially expressed proteins were involved in various cellular and biological processes.
Conclusion: This study provides a perspective on how cells change through biomechanics and a further theoretical foundation for the future application of Sophora moorcroftiana as a novel low-toxicity chemotherapy medication for treating human cervical cancer.
(© 2024. The Author(s).)
- References:
Nat Prod Res. 2018 Jun;32(12):1472-1475. (PMID: 28715918)
Antioxid Redox Signal. 2010 Jul 15;13(2):127-44. (PMID: 20014998)
Free Radic Biol Med. 2011 Jul 1;51(1):78-87. (PMID: 21539911)
Vet Res. 2018 Oct 4;49(1):100. (PMID: 30286809)
FEBS Lett. 2003 Mar 27;539(1-3):37-44. (PMID: 12650923)
Cancer Res. 2006 Jun 15;66(12):6080-6. (PMID: 16778180)
J Biol Chem. 2011 Mar 11;286(10):8394-8404. (PMID: 21148313)
Cancer Res. 2011 Jan 1;71(1):13-8. (PMID: 21199794)
Mol Cell Biochem. 2014 Feb;387(1-2):261-70. (PMID: 24234423)
Int J Clin Exp Pathol. 2015 Jun 01;8(6):6944-51. (PMID: 26261582)
Free Radic Biol Med. 2014 Jun;71:121-132. (PMID: 24681255)
Pharmacogn Mag. 2016 May;12(Suppl 2):S228-30. (PMID: 27279712)
Science. 1997 Oct 10;278(5336):294-8. (PMID: 9323209)
BMC Cancer. 2014 Feb 28;14:142. (PMID: 24581141)
Clin Cancer Res. 2013 Feb 15;19(4):773-84. (PMID: 23224736)
Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):393-407. (PMID: 31913710)
Nat Prod Res. 2021 Nov;35(22):4317-4322. (PMID: 31928368)
CA Cancer J Clin. 2024 May-Jun;74(3):229-263. (PMID: 38572751)
Chin J Nat Med. 2018 Apr;16(4):252-260. (PMID: 29703325)
Tumour Biol. 2014 Jan;35(1):195-204. (PMID: 23922175)
Hum Mol Genet. 2012 Jul 1;21(13):3013-24. (PMID: 22492997)
Neuropathology. 2011 Feb;31(1):29-37. (PMID: 20497343)
Cancer Res. 2012 Nov 15;72(22):5812-23. (PMID: 23139210)
Mol Med Rep. 2015 Aug;12(2):2003-8. (PMID: 25892179)
J Mol Med (Berl). 2004 Mar;82(3):163-74. (PMID: 14712351)
Cancer Sci. 2011 Nov;102(11):2007-13. (PMID: 21848913)
Chin J Nat Med. 2018 Sep;16(9):665-673. (PMID: 30269843)
J BUON. 2013 Apr-Jun;18(2):342-51. (PMID: 23818344)
Cold Spring Harb Perspect Biol. 2016 Aug 01;8(8):. (PMID: 26988969)
Phytother Res. 2014 Jul;28(7):1071-6. (PMID: 24338874)
Annu Rev Cell Dev Biol. 2020 Oct 6;36:35-60. (PMID: 33021819)
Int J Cancer. 2014 Nov 1;135(9):1991-6. (PMID: 24285383)
Carcinogenesis. 2012 Dec;33(12):2344-50. (PMID: 22962304)
Anticancer Res. 2001 Jan-Feb;21(1A):275-80. (PMID: 11299746)
PLoS One. 2015 May 01;10(5):e0123544. (PMID: 25932951)
Lancet Glob Health. 2020 Feb;8(2):e191-e203. (PMID: 31812369)
Mol Cancer. 2010 Apr 19;9:81. (PMID: 20403181)
Oncogene. 1997 Mar 6;14(9):1007-12. (PMID: 9070648)
Int J Oncol. 2012 Sep;41(3):985-94. (PMID: 22752059)
Int J Biochem Cell Biol. 2018 Jun;99:91-99. (PMID: 29627439)
Gynecol Oncol. 2011 Nov;123(2):314-9. (PMID: 21903246)
Cell Signal. 2014 Jun;26(6):1335-46. (PMID: 24607789)
Clin Cancer Res. 2000 Dec;6(12):4915-20. (PMID: 11156252)
Pathol Int. 2014 Apr;64(4):155-63. (PMID: 24750185)
Biomedicines. 2022 Jul 19;10(7):. (PMID: 35885046)
- Grant Information:
31920180129 Fundamental Research Funds for the Central universities of Northwestern Minzu University; 20JR10RA591 Natural Science Foundation of Gansu Province; 20JR10RA591 Natural Science Foundation of Gansu Province; 20JR10FA670 Clinical Research Center for Oral Diseases, the Scientific and Technological Foundation of Gansu Province; 2020-XG-36 Study on Cytoskeleton Biological Effect of novel coronavirus's Surface Adhesion Behavior
- Contributed Indexing:
Keywords: Sophora Moorcroftiana; Cell proliferation; Cervical cancer; Ethanol extract
- الرقم المعرف:
0 (Plant Extracts)
0 (Antineoplastic Agents, Phytogenic)
3K9958V90M (Ethanol)
- الموضوع:
Date Created: 20240603 Date Completed: 20240604 Latest Revision: 20240607
- الموضوع:
20240607
- الرقم المعرف:
PMC11149180
- الرقم المعرف:
10.1186/s12906-024-04502-5
- الرقم المعرف:
38831394
No Comments.