Long non-coding RNA MEG3 knockdown represses airway smooth muscle cells proliferation and migration via sponging miR-143-3p/FGF9 in asthma.

Publisher: BioMed Central Country of Publication: England NLM ID: 101265113 Publication Model: Electronic Cited Medium: Internet ISSN: 1749-8090 (Electronic) Linking ISSN: 17498090 NLM ISO Abbreviation: J Cardiothorac Surg Subsets: MEDLINE

Original Publication: [London] : BioMed Central, 2006-

RNA, Long Noncoding*/genetics ; RNA, Long Noncoding*/metabolism ; MicroRNAs*/genetics ; MicroRNAs*/metabolism ; Cell Movement*/physiology ; Cell Proliferation*/physiology ; Cell Proliferation*/genetics ; Asthma*/genetics ; Asthma*/metabolism ; Myocytes, Smooth Muscle*/metabolism ; Fibroblast Growth Factor 9*/genetics ; Fibroblast Growth Factor 9*/metabolism; Humans ; Cells, Cultured ; Airway Remodeling/physiology ; Airway Remodeling/genetics

Background: Asthma is a respiratory disease characterized by airway remodeling. We aimed to find out the role and mechanism of lncRNA MEG3 in asthma.
Methods: We established a cellular model of asthma by inducing human airway smooth muscle cells (HASMCs) with PDGF-BB, and detected levels of lncRNA MEG3, miR-143-3p and FGF9 in HASMCs through qRT-PCR. The functions of lncRNA MEG3 or miR-143-3p on HASMCs were explored by cell transfection. The binding sites of miR-143-3p and FGF9 were subsequently analyzed with bioinformatics software, and validated with dual-luciferase reporter assay. MTT, 5-Ethynyl-2'-deoxyuridine (EdU) assay, and Transwell were used to detect the effects of lncRNA MEG3 or miR-143-3p on proliferation and migration of HASMCs. QRT-PCR and western blot assay were used to evaluate the level of proliferation-related marker PCNA in HASMCs.
Results: The study found that lncRNA MEG3 negatively correlated with miR-143-3p, and miR-143-3p could directly target with FGF9. Silence of lncRNA MEG3 can suppress migration and proliferation of PDGF-BB-induced HASMCs via increasing miR-143-3p. Further mechanistic studies revealed that miR-143-3p negatively regulated FGF9 expression in HASMCs. MiR-143-3p could inhibit PDGF-BB-induced HASMCs migration and proliferation through downregulating FGF9.
Conclusion: LncRNA MEG3 silencing could inhibit the migration and proliferation of HASMCs through regulating miR-143-3p/FGF9 signaling axis. These results imply that lncRNA MEG3 plays a protective role against asthma.
(© 2024. The Author(s).)

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Keywords: Airway smooth muscle cells; Asthma; Migration; Proliferation; lncRNA MEG3; miR-143-3p/FGF9 axis

0 (RNA, Long Noncoding)
0 (MicroRNAs)
0 (Fibroblast Growth Factor 9)
0 (MEG3 non-coding RNA, human)
0 (MIRN143 microRNA, human)
0 (FGF9 protein, human)

Date Created: 20240602 Date Completed: 20240602 Latest Revision: 20240611

20240611

PMC11143653

10.1186/s13019-024-02798-5

38824534