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Long non-coding RNA MEG3 knockdown represses airway smooth muscle cells proliferation and migration via sponging miR-143-3p/FGF9 in asthma.
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- المؤلفون: Gu J;Gu J; Zhou D; Zhou D
- المصدر:
Journal of cardiothoracic surgery [J Cardiothorac Surg] 2024 Jun 01; Vol. 19 (1), pp. 314. Date of Electronic Publication: 2024 Jun 01.
- نوع النشر :
Journal Article
- اللغة:
English
- معلومة اضافية
- المصدر:
Publisher: BioMed Central Country of Publication: England NLM ID: 101265113 Publication Model: Electronic Cited Medium: Internet ISSN: 1749-8090 (Electronic) Linking ISSN: 17498090 NLM ISO Abbreviation: J Cardiothorac Surg Subsets: MEDLINE
- بيانات النشر:
Original Publication: [London] : BioMed Central, 2006-
- الموضوع:
- نبذة مختصرة :
Background: Asthma is a respiratory disease characterized by airway remodeling. We aimed to find out the role and mechanism of lncRNA MEG3 in asthma.
Methods: We established a cellular model of asthma by inducing human airway smooth muscle cells (HASMCs) with PDGF-BB, and detected levels of lncRNA MEG3, miR-143-3p and FGF9 in HASMCs through qRT-PCR. The functions of lncRNA MEG3 or miR-143-3p on HASMCs were explored by cell transfection. The binding sites of miR-143-3p and FGF9 were subsequently analyzed with bioinformatics software, and validated with dual-luciferase reporter assay. MTT, 5-Ethynyl-2'-deoxyuridine (EdU) assay, and Transwell were used to detect the effects of lncRNA MEG3 or miR-143-3p on proliferation and migration of HASMCs. QRT-PCR and western blot assay were used to evaluate the level of proliferation-related marker PCNA in HASMCs.
Results: The study found that lncRNA MEG3 negatively correlated with miR-143-3p, and miR-143-3p could directly target with FGF9. Silence of lncRNA MEG3 can suppress migration and proliferation of PDGF-BB-induced HASMCs via increasing miR-143-3p. Further mechanistic studies revealed that miR-143-3p negatively regulated FGF9 expression in HASMCs. MiR-143-3p could inhibit PDGF-BB-induced HASMCs migration and proliferation through downregulating FGF9.
Conclusion: LncRNA MEG3 silencing could inhibit the migration and proliferation of HASMCs through regulating miR-143-3p/FGF9 signaling axis. These results imply that lncRNA MEG3 plays a protective role against asthma.
(© 2024. The Author(s).)
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- Contributed Indexing:
Keywords: Airway smooth muscle cells; Asthma; Migration; Proliferation; lncRNA MEG3; miR-143-3p/FGF9 axis
- الرقم المعرف:
0 (RNA, Long Noncoding)
0 (MicroRNAs)
0 (Fibroblast Growth Factor 9)
0 (MEG3 non-coding RNA, human)
0 (MIRN143 microRNA, human)
0 (FGF9 protein, human)
- الموضوع:
Date Created: 20240602 Date Completed: 20240602 Latest Revision: 20240611
- الموضوع:
20240611
- الرقم المعرف:
PMC11143653
- الرقم المعرف:
10.1186/s13019-024-02798-5
- الرقم المعرف:
38824534
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