Unveiling the Role of RNA Recognition Motif Proteins in Orchestrating Nucleotide-Binding Site and Leucine-Rich Repeat Protein Gene Pairs and Chloroplast Immunity Pathways: Insights into Plant Defense Mechanisms.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Chloroplasts*/metabolism ; Chloroplasts*/genetics ; Plant Immunity*/genetics ; Plant Proteins*/genetics ; Plant Proteins*/metabolism ; Gene Expression Regulation, Plant* ; Oryza*/genetics ; Oryza*/metabolism ; Oryza*/immunology; Leucine-Rich Repeat Proteins ; Binding Sites ; RNA Recognition Motif Proteins/metabolism ; RNA Recognition Motif Proteins/genetics ; Plant Diseases/genetics ; Plant Diseases/immunology ; Disease Resistance/genetics ; NLR Proteins/metabolism ; NLR Proteins/genetics ; RNA Editing

In plants, nucleotide-binding site and leucine-rich repeat proteins (NLRs) play pivotal roles in effector-triggered immunity (ETI). However, the precise mechanisms underlying NLR-mediated disease resistance remain elusive. Previous studies have demonstrated that the NLR gene pair Pik-H4 confers resistance to rice blast disease by interacting with the transcription factor OsBIHD1, consequently leading to the upregulation of hormone pathways. In the present study, we identified an RNA recognition motif (RRM) protein, OsRRM2, which interacted with Pik 1 -H4 and Pik 2 -H4 in vesicles and chloroplasts. OsRRM2 exhibited a modest influence on Pik-H4 -mediated rice blast resistance by upregulating resistance genes and genes associated with chloroplast immunity. Moreover, the RNA-binding sequence of OsRRM2 was elucidated using systematic evolution of ligands by exponential enrichment. Transcriptome analysis further indicated that OsRRM2 promoted RNA editing of the chloroplastic gene ndhB . Collectively, our findings uncovered a chloroplastic RRM protein that facilitated the translocation of the NLR gene pair and modulated chloroplast immunity, thereby bridging the gap between ETI and chloroplast immunity.

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CARS-01-18 National Rice Industry Technology System Construction; 2023A1515012101 Guangdong Basic and Applied Basic Research Foundation, China; 2022YFD1200703 National Key Research and Development Plan, China

Keywords: NLRs; RNA recognition motif; chloroplast immunity; rice blast

0 (Plant Proteins)
0 (Leucine-Rich Repeat Proteins)
0 (RNA Recognition Motif Proteins)
0 (NLR Proteins)

Date Created: 20240525 Date Completed: 20240525 Latest Revision: 20240527

20240527

PMC11122538

10.3390/ijms25105557

38791594