Root exudates and rhizosphere microbiota in responding to long-term continuous cropping of tobacco.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Rhizosphere* ; Nicotiana*/microbiology ; Nicotiana*/growth & development ; Plant Roots*/microbiology ; Plant Roots*/growth & development ; Microbiota* ; Soil Microbiology*; Plant Exudates/metabolism ; Soil/chemistry

Soil sickness a severe problem in tobacco production, leading to soil-borne diseases and reduce in tobacco yield. This occurs as a result of the interaction between root exudates and rhizosphere microorganisms, which is however, little studied until now. By combining the field investigation and pot experiment, we found the output yield consistently decreased during the first 10 years of continuous cropping in a tobacco field, but increased at the 15th year (15Y). The root exudate and rhizosphere bacterial community was further analyzed to reveal the underlying mechanism of the suppressive soil formation. Root exudate of 15Y tobacco enriched in amino acids and derivatives, while depleted in the typical autotoxins including phenolic acids and alkaloids. This was correlated to the low microbial diversity in 15Y, but also the changes in community composition and topological properties of the co-occurrence network. Especially, the reduced autotoxins were associated with low Actinobacteria abundance, low network complexity and high network modularity, which significantly correlated with the recovered output yield in 15Y. This study revealed the coevolution of rhizosphere microbiota and root exudate as the soil domesticated by continuous cropping of tobacco, and indicated a potential role of the autotoxins and theirs effect on the microbial community in the formation of suppressive soil.
(© 2024. The Author(s).)

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202001AU070006 Yunnan Science and Technology key research project; 2019530000241024 Yunnan Academy of Tobacco Agricultural Sciences; 2021530000241008 Yunnan Academy of Tobacco Agricultural Sciences; 42207262 National Natural Science Foundation of China

Keywords: Co-occurrence network; Continuous cropping; Microbial community; Root exudate; Suppressive soil

Date Created: 20240517 Date Completed: 20240517 Latest Revision: 20240521

20240521

PMC11101450

10.1038/s41598-024-61291-0

38760388