Muscle Psn gene combined with exercise contribute to healthy aging of skeletal muscle and lifespan by adaptively regulating Sirt1/PGC-1α and arm pathway.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Longevity* ; Muscle, Skeletal*/metabolism ; Muscle, Skeletal*/physiology ; Physical Conditioning, Animal* ; Signal Transduction*; Animals ; Aging/physiology ; Aging/genetics ; Aging/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Healthy Aging/genetics ; Healthy Aging/metabolism ; Healthy Aging/physiology ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/metabolism ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/genetics ; Sirtuin 1/metabolism ; Sirtuin 1/genetics ; Transcription Factors/metabolism ; Transcription Factors/genetics

The Presenilin (Psn) gene is closely related to aging, but it is still unclear the role of Psn genes in skeletal muscle. Here, the Psn-UAS/Mhc-GAL4 system in Drosophila was used to regulate muscle Psn overexpression(MPO) and muscle Psn knockdown(MPK). Drosophila were subjected to endurance exercise from 4 weeks to 5 weeks old. The results showed that MPO and exercise significantly increased climbing speed, climbing endurance, lifespan, muscle SOD activity, Psn expression, Sirt1 expression, PGC-1α expression, and armadillo (arm) expression in aged Drosophila, and they significantly decreased muscle malondialdehyde levels. Interestingly, when the Psn gene is knockdown by 0.78 times, the PGC-1α expression and arm expression were also down-regulated, but the exercise capacity and lifespan were increased. Furthermore, exercise combined with MPO further improved the exercise capacity and lifespan. MPK combined with exercise further improves the exercise capacity and lifespan. Thus, current results confirmed that the muscle Psn gene was a vital gene that contributed to the healthy aging of skeletal muscle since whether it was overexpressed or knocked down, the aging progress of skeletal muscle structure and function was slowed down by regulating the activity homeostasis of Sirt1/PGC-1α pathway and Psn/arm pathway. Exercise enhanced the function of the Psn gene to delay skeletal muscle aging by up regulating the activity of the Sirt1/PGC-1α pathway and Psn/arm pathway.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

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0 (Drosophila Proteins)
0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
EC 3.5.1.- (Sirtuin 1)
0 (Transcription Factors)
0 (Psn protein, Drosophila)

Date Created: 20240516 Date Completed: 20240516 Latest Revision: 20240613

20250114

PMC11098322

10.1371/journal.pone.0300787

38753634