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Riboswitch and small RNAs modulate btuB translation initiation in Escherichia coli and trigger distinct mRNA regulatory mechanisms.
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- معلومة اضافية
- المصدر:
Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
- بيانات النشر:
Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
- الموضوع:
- نبذة مختصرة :
Small RNAs (sRNAs) and riboswitches represent distinct classes of RNA regulators that control gene expression upon sensing metabolic or environmental variations. While sRNAs and riboswitches regulate gene expression by affecting mRNA and protein levels, existing studies have been limited to the characterization of each regulatory system in isolation, suggesting that sRNAs and riboswitches target distinct mRNA populations. We report that the expression of btuB in Escherichia coli, which is regulated by an adenosylcobalamin (AdoCbl) riboswitch, is also controlled by the small RNAs OmrA and, to a lesser extent, OmrB. Strikingly, we find that the riboswitch and sRNAs reduce mRNA levels through distinct pathways. Our data show that while the riboswitch triggers Rho-dependent transcription termination, sRNAs rely on the degradosome to modulate mRNA levels. Importantly, OmrA pairs with the btuB mRNA through its central region, which is not conserved in OmrB, indicating that these two sRNAs may have specific targets in addition to their common regulon. In contrast to canonical sRNA regulation, we find that OmrA repression of btuB is lost using an mRNA binding-deficient Hfq variant. Together, our study demonstrates that riboswitch and sRNAs modulate btuB expression, providing an example of cis- and trans-acting RNA-based regulatory systems maintaining cellular homeostasis.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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- Grant Information:
PJT153205 Canada CAPMC CIHR; PJT153205 Canada CAPMC CIHR; RGPIN-2020-06241 Natural Sciences and Engineering Research Council of Canada; 818750 International ERC_ European Research Council; 101034407 Marie Skłodowska-Curie; CNRS; DYNAMO FrenchState
- الرقم المعرف:
0 (Riboswitch)
0 (Escherichia coli Proteins)
0 (RNA, Messenger)
0 (Cobamides)
0 (BtuB protein, E coli)
F0R1QK73KB (cobamamide)
0 (RNA, Bacterial)
0 (RNA, Small Untranslated)
0 (degradosome)
EC 3.6.4.13 (RNA Helicases)
EC 3.1.- (Endoribonucleases)
0 (Multienzyme Complexes)
0 (Bacterial Outer Membrane Proteins)
EC 2.7.7.8 (Polyribonucleotide Nucleotidyltransferase)
0 (Membrane Transport Proteins)
- الموضوع:
Date Created: 20240514 Date Completed: 20240609 Latest Revision: 20240613
- الموضوع:
20250114
- الرقم المعرف:
PMC11162775
- الرقم المعرف:
10.1093/nar/gkae347
- الرقم المعرف:
38742638
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