A high-affinity potassium transporter (MeHKT1) from cassava (Manihot esculenta) negatively regulates the response of transgenic Arabidopsis to salt stress.

Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Arabidopsis*/genetics ; Arabidopsis*/physiology ; Arabidopsis*/metabolism ; Manihot*/genetics ; Manihot*/metabolism ; Manihot*/physiology ; Plants, Genetically Modified*/genetics ; Potassium*/metabolism ; Salt Stress*/genetics ; Plant Proteins*/genetics ; Plant Proteins*/metabolism; Cation Transport Proteins/genetics ; Cation Transport Proteins/metabolism ; Gene Expression Regulation, Plant ; Salt Tolerance/genetics ; Sodium/metabolism

Background: High-affinity potassium transporters (HKTs) are crucial in facilitating potassium uptake by plants. Many types of HKTs confer salt tolerance to plants through regulating K ⁺ and Na ⁺ homeostasis under salinity stress. However, their specific functions in cassava (Manihot esculenta) remain unclear.
Results: Herein, an HKT gene (MeHKT1) was cloned from cassava, and its expression is triggered by exposure to salt stress. The expression of a plasma membrane-bound protein functions as transporter to rescue a low potassium (K ⁺ ) sensitivity of yeast mutant strain, but the complementation of MeHKT1 is inhibited by NaCl treatment. Under low K ⁺ stress, transgenic Arabidopsis with MeHKT1 exhibits improved growth due to increasing shoot K ⁺ content. In contrast, transgenic Arabidopsis accumulates more Na ⁺ under salt stress than wild-type (WT) plants. Nevertheless, the differences in K ⁺ content between transgenic and WT plants are not significant. Additionally, Arabidopsis expressing MeHKT1 displayed a stronger salt-sensitive phenotype.
Conclusion: These results suggest that under low K ⁺ condition, MeHKT1 functions as a potassium transporter. In contrast, MeHKT1 mainly transports Na ⁺ into cells under salt stress condition and negatively regulates the response of transgenic Arabidopsis to salt stress. Our results provide a reference for further research on the function of MeHKT1, and provide a basis for further application of MeHKT1 in cassava by molecular biological means.
(© 2024. The Author(s).)

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Hnky2021-19 The Education Department of Hainan Province; 2023A1515012295 Natural Science Foundation of Guangdong Province; 2018YFE0207203-2 National Key R&D Program of China

Keywords: Cassava; High-affinity potassium transporter; Potassium starvation; Salt tolerance

RWP5GA015D (Potassium)
0 (Plant Proteins)
0 (Cation Transport Proteins)
9NEZ333N27 (Sodium)

Date Created: 20240507 Date Completed: 20240508 Latest Revision: 20240528

20240529

PMC11075273

10.1186/s12870-024-05084-7

38714917