Prevalence and molecular characterization of colistin resistance in Pseudomonas aeruginosa isolates: insights from a study in Ardabil hospitals.

Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Colistin*/pharmacology ; Pseudomonas aeruginosa*/genetics ; Pseudomonas aeruginosa*/drug effects ; Pseudomonas aeruginosa*/isolation & purification ; Anti-Bacterial Agents*/pharmacology ; Microbial Sensitivity Tests* ; Bacterial Proteins*/genetics ; Pseudomonas Infections*/microbiology ; Pseudomonas Infections*/epidemiology ; Transcription Factors*; Humans ; Prevalence ; Drug Resistance, Multiple, Bacterial/genetics ; Biofilms/drug effects ; Biofilms/growth & development ; Hospitals ; Drug Resistance, Bacterial/genetics ; Cross Infection/microbiology ; Cross Infection/epidemiology ; Membrane Transport Proteins/genetics ; Porins/genetics

Background: Pseudomonas aeruginosa is a common cause of nosocomial infections. However, the emergence of multidrug-resistant strains has complicated the treatment of P. aeruginosa infections. While polymyxins have been the mainstay for treatment, there is a global increase in resistance to these antibiotics. Therefore, our study aimed to determine the prevalence and molecular details of colistin resistance in P. aeruginosa clinical isolates collected between June 2019 and May 2023, as well as the genetic linkage of colistin-resistant P. aeruginosa isolates.
Results: The resistance rate to colistin was 9% (n = 18) among P. aeruginosa isolates. All 18 colistin-resistant isolates were biofilm producers and carried genes associated with biofilm formation. Furthermore, the presence of genes encoding efflux pumps, TCSs, and outer membrane porin was observed in all colistin-resistant P. aeruginosa strains, while the mcr-1 gene was not detected. Amino acid substitutions were identified only in the PmrB protein of multidrug- and colistin-resistant strains. The expression levels of mexA, mexC, mexE, mexY, phoP, and pmrA genes in the 18 colistin-resistant P. aeruginosa strains were as follows: 88.8%, 94.4%, 11.1%, 83.3%, 83.3%, and 38.8%, respectively. Additionally, down-regulation of the oprD gene was observed in 44.4% of colistin-resistant P. aeruginosa strains.
Conclusion: This study reports the emergence of colistin resistance with various mechanisms among P. aeruginosa strains in Ardabil hospitals. We recommend avoiding unnecessary use of colistin to prevent potential future increases in colistin resistance.
(© 2024. The Author(s).)

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401000409 Ardabil University of Medical Sciences, Iran; 401000409 Ardabil University of Medical Sciences, Iran; 401000409 Ardabil University of Medical Sciences, Iran; 401000409 Ardabil University of Medical Sciences, Iran; 401000409 Ardabil University of Medical Sciences, Iran; 401000409 Ardabil University of Medical Sciences, Iran

Keywords: Pseudomonas aeruginosa; Colistin; Multidrug-resistant

Z67X93HJG1 (Colistin)
0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)
0 (PmrB protein, bacteria)
0 (Membrane Transport Proteins)
0 (Porins)
0 (Transcription Factors)

Date Created: 20240503 Date Completed: 20240504 Latest Revision: 20240507

20240507

PMC11067120

10.1186/s12866-024-03309-1

38702660