Omentin-1 May Be One Treatment Factor for Intravenous Thrombolysis of Acute Cerebral Infarction Through the Inhibition of NLRP3 Ubiquitination by AMPK Function: Preliminary Findings.

Publisher: Medknow Publications Country of Publication: India NLM ID: 0042005 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1998-4022 (Electronic) Linking ISSN: 00283886 NLM ISO Abbreviation: Neurol India Subsets: MEDLINE

Publication: Mumbai : Medknow Publications
Original Publication: Bombay : Neurological Society of India

Cytokines*/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein*/metabolism ; GPI-Linked Proteins*/metabolism ; Lectins* ; Ubiquitination*/drug effects ; Mice, Inbred C57BL*; Animals ; Male ; Mice ; Humans ; Disease Models, Animal ; Cerebral Infarction/drug therapy ; AMP-Activated Protein Kinases/metabolism ; Thrombolytic Therapy/methods ; Middle Aged ; Aged

Background: Acute cerebral infarction (ACI) is a common neurological disease that is associated with high morbidity, disability and mortality rates. At present, antiplatelet therapy is a necessary treatment for ACI. The present study aimed to investigate the effects of omentin-1 on the intravenous thrombolysis of ACI.
Objective: The present study aimed to investigate the effects of omentin-1 on the intravenous thrombolysis of ACI.
Material and Methods: The mouse model of ACI was induced using male C57BL/6 mice through middle cerebral artery occlusion (MCAO). Meanwhile, the murine BV2 microglial cells were pretreated with 0.1 mg/ml of lipopolysaccharide (LPS), and then induced with 2 mM of adenosine triphosphate (ATP).
Results: The omentin-1 mRNA expression in patients receiving intravenous thrombolysis for ACI was down-regulated compared with the normal group. Additionally, the serum level of omentin-1 was negatively correlated with National Institute of Health Stroke Scale (NIHSS) score or serum level of IL-1β or MMP-2 in patients receiving intravenous thrombolysis for ACI. Meanwhile, the serum mRNA expression of omentin-1 was positively correlated with Barthel index or high-sensitivity C-reactive protein (hs-CRP) in patients undergoing intravenous thrombolysis for ACI. As observed from the in vitro model, Omentin-1 reduced inflammation, promoted cell growth, alleviated ROS-induced oxidative stress, and enhanced AMPK activity through activating NLRP3 ubiquitination. Omentin-1 presented ACI in the mouse model of ACI. Regulating AMPK activity contributed to controlling the effects of Omentin-1 on the in vitro model.
Conclusions: Omentin-1 reduced neuroinflammation and ROS-induced oxidative stress in the mouse model of ACI, which was achieved by inhibiting NLRP3 ubiquitination through regulating AMPK activity. Therefore, omentin-1 may serve as a treatment factor for the intravenous thrombolysis of ACI in further clinical application.
(Copyright © 2024 Copyright: © 2024 Neurology India, Neurological Society of India.)

AlGhadeer H, Talea M, Al-Muhaylib A, AlSheikh O, Elkhamary SM. Acute unilateral vision loss and bilateral cerebral infarction following cosmetic filler injection. Orbit 2021;1–4. doi: 10.1080/01676830.2021.1974056.
Arboix A, Jiménez C, Massons J, Parra O, Besses C. Hematological disorders: A commonly unrecognized cause of acute stroke. Expert Rev Hematol 2016;9:891–901.
Koizumi K, Sato H, Ebitani M, Kaneko K, Oguchi K, Hashimoto T. Acute cerebral microbleeds at the edge of lacunar strokes: Cause or result. Neuroradiol J 2021;35:408–11. 19714009211042878.
Chihi M, Darkwah Oppong M, Pierscianek D, Dinger TF, Ahmadipour Y, et al. Analysis of brain natriuretic peptide levels following traumatic acute subdural hematoma and the risk of postoperative cerebral infarction. J Neurotrauma 2021;38:3068–76.
Kishimoto M, Suenaga J, Takase H, Araki K, Yao T, et al. Oxidative stress-responsive apoptosis inducing protein (ORAIP) plays a critical role in cerebral ischemia/reperfusion injury. Sci Rep 2019;9:13512.
Liu H, Wu X, Luo J, Zhao L, Li X, Guo H, et al. Adiponectin peptide alleviates oxidative stress and NLRP3 inflammasome activation after cerebral ischemia-reperfusion injury by regulating AMPK/GSK-3β. Exp Neurol 2020;329:113302.
Lorenzano S, Rost NS, Khan M, Li H, Batista LM, Chutinet A, et al. Early molecular oxidative stress biomarkers of ischemic penumbra in acute stroke. Neurology 2019;93:e1288–98.
Franke M, Bieber M, Kraft P, Weber ANR, Stoll G, Schuhmann MK. The NLRP3 inflammasome drives inflammation in ischemia/reperfusion injury after transient middle cerebral artery occlusion in mice. Brain Behav Immun 2021;92:223–33.
Hou Y, Wang Y, He Q, Li L, Xie H, Zhao Y, et al. Nrf2 inhibits NLRP3 inflammasome activation through regulating Trx1/TXNIP complex in cerebral ischemia reperfusion injury. Behav Brain Res 2018;336:32–39.
Yang B, Sun Y, Lv C, Zhang W, Chen Y. Procyanidins exhibits neuroprotective activities against cerebral ischemia reperfusion injury by inhibiting TLR4-NLRP3 inflammasome signal pathway. Psychopharmacology (Berl) 2020;237:3283–93.
Bai P, Abdullah F, Lodi M, Sarhadi M, Dilip A, Shahab S, et al. Association between coronary artery disease and plasma omentin-1 levels. Cureus 2021;13:e17347.
González-López MA, Ocejo-Viñals JG, Mata C, Guiral S, Ruiz-Solana M, Mata C, et al. Evaluation of serum omentin-1 and apelin concentrations in patients with hidradenitis suppurativa. Postepy Dermatol Alergol 2021;38:450–4.
Jin Z, Xia F, Dong J, Lin T, Cai Y, Chen J, et al. Omentin-1 attenuates glucocorticoid-induced cardiac injury by phosphorylating GSK3β. J Mol Endocrinol 2021;66:273–83.
Lin S, Li X, Zhang J, Zhang Y. Omentin-1: Protective impact on ischemic stroke via ameliorating atherosclerosis. Clin Chim Acta 2021;517:31–40.
Fang B, Zhai H. Adoption of computerized tomography perfusion imaging in the diagnosis of acute cerebral infarct under optimized deconvolution algorithm. Pak J Med Sci 2021;37:1687–92.
Seo WK, Nam HS, Chung JW, Kim YD, Kim KH, Bang OY, et al. TAB-TICI score: Successful recanalization score after endovascular thrombectomy in acute stroke. Front Neurol 2021;12:692490.
Li Y, Lin Y, Shi Z, Hu Y, Shao T, Li M, et al. Safflower yellow pigment and Sanqi Panax notoginseng in the treatment of acute cerebral infarction: A systematic review, meta-analysis, and cost-effectiveness analysis. Ann Transl Med 2021;9:1407.
Chen LL, Wang WT, Zhang S, Liu H-M, Yuan X-Y, Yang X, et al. Cohort study on the prognosis of acute cerebral infarction in different circulatory systems at 1-year follow-up. BMC Cardiovasc Disord 2021;21:521.
Yang J, Gao Y. Clinical relevance of serum omentin-1 levels as a biomarker of prognosis in patients with acute cerebral infarction. Brain Behav 2020;10:e01678.
Yan W, Sun W, Fan J, Wang H, Han S, Li J, et al. Sirt1-ROS-TRAF6 Signaling-induced pyroptosis contributes to early injury in ischemic mice. Neurosci Bull 2020;36:845–59.
Zhang F, Chen S, Wen JY, Chen ZW. 3-Mercaptopyruvate sulfurtransferase/hydrogen sulfide protects cerebral endothelial cells against oxygen-glucose deprivation/reoxygenation-induced injury via mitoprotection and inhibition of the RhoA/ROCK pathway. Am J Physiol Cell Physiol 2020;319:C720–33.
Wu Y, Yao J, Feng K. miR-124-5p/NOX2 axis modulates the ROS production and the inflammatory microenvironment to protect against the cerebral I/R injury. Neurochem Res 2020;45:404–17.
Rao SS, Hu Y, Xie PL, Cao J, Wang Z-X, Liu J-H, et al. Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines. Bone Res 2019;6:9.
Liu F, Fang S, Liu X, Li J, Wang X, Cui J, et al. Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway. Biochem Pharmacol 2020;174:113830.
Zhang W, Wang W, Shen C, Wang X, Pu Z, Yin Q. Network pharmacology for systematic understanding of Schisandrin B reduces the epithelial cells injury of colitis through regulating pyroptosis by AMPK/Nrf2/NLRP3 inflammasome. Aging (Albany NY) 2021;13:23193–209.
Zhang W, Wang W, Xu M, Xie H, Pu Z. GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis. Aging (Albany NY) 2021;13:22588–610.
Rudilosso S, Rodríguez-Vázquez A, Urra X, Arboix A. The potential impact of neuroimaging and translational research on the clinical management of lacunar stroke. Int J Mol Sci 2022;23:1497.

0 (Cytokines)
0 (NLR Family, Pyrin Domain-Containing 3 Protein)
0 (GPI-Linked Proteins)
0 (Lectins)
0 (ITLN1 protein, human)
EC 2.7.11.31 (AMP-Activated Protein Kinases)
0 (Itln1 protein, mouse)
0 (Nlrp3 protein, mouse)

Date Created: 20240501 Date Completed: 20240501 Latest Revision: 20240702

20240702

10.4103/ni.ni_1325_21

38691475