Redox active plant phenolic, acetosyringone, for electrogenetic signaling.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Oxidation-Reduction* ; Signal Transduction* ; Escherichia coli*/genetics ; Escherichia coli*/metabolism; Hydrogen Peroxide ; Regulon/genetics ; Gene Expression Regulation, Bacterial ; Escherichia coli Proteins/metabolism ; Escherichia coli Proteins/genetics ; Phenols/chemistry ; Phenols/metabolism

Redox is a unique, programmable modality capable of bridging communication between biology and electronics. Previous studies have shown that the E. coli redox-responsive OxyRS regulon can be re-wired to accept electrochemically generated hydrogen peroxide (H 2 O 2 ) as an inducer of gene expression. Here we report that the redox-active phenolic plant signaling molecule acetosyringone (AS) can also induce gene expression from the OxyRS regulon. AS must be oxidized, however, as the reduced state present under normal conditions cannot induce gene expression. Thus, AS serves as a "pro-signaling molecule" that can be activated by its oxidation-in our case by application of oxidizing potential to an electrode. We show that the OxyRS regulon is not induced electrochemically if the imposed electrode potential is in the mid-physiological range. Electronically sliding the applied potential to either oxidative or reductive extremes induces this regulon but through different mechanisms: reduction of O 2 to form H 2 O 2 or oxidation of AS. Fundamentally, this work reinforces the emerging concept that redox signaling depends more on molecular activities than molecular structure. From an applications perspective, the creation of an electronically programmed "pro-signal" dramatically expands the toolbox for electronic control of biological responses in microbes, including in complex environments, cell-based materials, and biomanufacturing.
(© 2024. The Author(s).)

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1932963 Division of Chemical, Bioengineering, Environmental, and Transport Systems; 2227598 Division of Molecular and Cellular Biosciences; SCW1710 Biological and Environmental Research; HDTRA1-19-1-0021 Defense Threat Reduction Agency; 11395 Gordon and Betty Moore Foundation; 1840340 Division of Graduate Education

BBX060AN9V (Hydrogen Peroxide)
0 (Escherichia coli Proteins)
0 (Phenols)

Date Created: 20240426 Date Completed: 20240426 Latest Revision: 20240429

20240429

PMC11053109

10.1038/s41598-024-60191-7

38671069