Detection of bla KPC gene among carbapenemase producing Klebsiella pneumoniae isolated from different clinical specimens at tertiary care hospital of Nepal.

Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE

Original Publication: London : BioMed Central, [2001-

Klebsiella pneumoniae*/genetics ; Klebsiella pneumoniae*/drug effects ; Klebsiella pneumoniae*/isolation & purification ; Klebsiella pneumoniae*/enzymology ; beta-Lactamases*/genetics ; Klebsiella Infections*/microbiology ; Klebsiella Infections*/epidemiology ; Tertiary Care Centers*/statistics & numerical data ; Bacterial Proteins*/genetics ; Anti-Bacterial Agents*/pharmacology ; Microbial Sensitivity Tests* ; Meropenem*/pharmacology; Humans ; Nepal/epidemiology ; Cross-Sectional Studies ; Prospective Studies ; Male ; Drug Resistance, Multiple, Bacterial/genetics ; Female ; Adult ; Middle Aged ; Young Adult ; Aged ; Adolescent

Background: Klebsiella pneumoniae infections have become a major cause of hospital acquired infection worldwide with the increased rate of acquisition of resistance to antibiotics. Carbapenem resistance mainly among Gram negative is an ongoing problem which causes serious outbreaks dramatically limiting treatment options. This prospective cross-sectional study was designed to detect bla KPC gene from carbapenem resistant K. pneumoniae.
Materials and Methods: A totally of 1118 different clinical specimens were screened and confirmed for KPC producing K. pneumoniae phenotypically using Meropenem (10 μg) disc. The bla KPC gene was amplified from the isolates of K. pneumoniae to detect the presence of this gene.
Result: Of the total samples processed, 18.6% (n = 36) were K. pneumoniae and among 36 K. pneumoniae, 61.1% (n = 22/36) were meropenem resistant. This study demonstrated the higher level of MDR 91.7% (n = 33) and KPC production 47.2% (n = 17) among K. pneumoniae isolates. The bla KPC gene was detected in 8.3% (n = 3) of meropenem resistant isolates.
Conclusion: Since the study demonstrates the higher level of MDR and KPC producing K. pneumoniae isolates that has challenged the use of antimicrobial agents, continuous microbiology, and molecular surveillance to assist early detection and minimize the further dissemination of bla KPC should be initiated. We anticipate that the findings of this study will be useful in understanding the prevalence of KPC-producing K. pneumoniae in Nepal.
(© 2024. The Author(s).)

Int J Clin Pract. 2020 Apr;74(4):e13463. (PMID: 31830351)
Antimicrob Agents Chemother. 2015 Nov 09;60(1):669-73. (PMID: 26552970)
Emerg Infect Dis. 2011 Oct;17(10):1791-8. (PMID: 22000347)
Infect Drug Resist. 2020 Jul 14;13:2311-2321. (PMID: 32765007)
Oman Med J. 2011 Sep;26(5):368-70. (PMID: 22216389)
Front Med (Lausanne). 2019 May 24;6:105. (PMID: 31179281)
Antimicrob Agents Chemother. 2014;58(2):654-63. (PMID: 24080646)
Interdiscip Perspect Infect Dis. 2012;2012:976273. (PMID: 23097666)
Cold Spring Harb Perspect Med. 2017 Jan 3;7(1):. (PMID: 27742735)
N Engl J Med. 2009 Jan 29;360(5):439-43. (PMID: 19179312)
Am J Infect Control. 2005 Aug;33(6):326-32. (PMID: 16061138)
Antimicrob Agents Chemother. 2015 Oct;59(10):5873-84. (PMID: 26169401)
MMWR Morb Mortal Wkly Rep. 2013 Mar 8;62(9):165-70. (PMID: 23466435)
Lancet Infect Dis. 2009 Apr;9(4):228-36. (PMID: 19324295)
Infect Drug Resist. 2018 Aug 20;11:1219-1224. (PMID: 30154668)
J Antimicrob Chemother. 2010 Jul;65(7):1319-21. (PMID: 20395214)
Future Microbiol. 2007 Oct;2(5):501-12. (PMID: 17927473)
Ann Clin Microbiol Antimicrob. 2017 Sep 19;16(1):62. (PMID: 28927454)
Clin Microbiol Infect. 2008 Oct;14(10):895-907. (PMID: 18828851)
J Exp Med. 2016 Aug 22;213(9):1851-64. (PMID: 27526712)
Trop Med Health. 2021 Mar 11;49(1):23. (PMID: 33691795)
Trends Microbiol. 2014 Dec;22(12):686-96. (PMID: 25304194)
Iran J Pathol. 2015 Summer;10(3):199-205. (PMID: 26351485)
Nepal Med Coll J. 2011 Mar;13(1):34-8. (PMID: 21991699)
Nepal Med Coll J. 2008 Jun;10(2):86-90. (PMID: 18828428)
J Clin Microbiol. 2009 Feb;47(2):362-7. (PMID: 19073868)
Antimicrob Agents Chemother. 2001 Apr;45(4):1151-61. (PMID: 11257029)
Clin Infect Dis. 2002 Mar 1;34(5):634-40. (PMID: 11823954)
South Med J. 2011 Jan;104(1):40-5. (PMID: 21119555)
Drug Res (Stuttg). 2018 Jul;68(7):415-420. (PMID: 29529677)
Diagn Microbiol Infect Dis. 2011 Jun;70(2):274-7. (PMID: 21397425)
BMC Res Notes. 2012 Jan 19;5:38. (PMID: 22260454)
Clin Microbiol Infect. 2011 Apr;17(4):552-6. (PMID: 20597925)
JNMA J Nepal Med Assoc. 2016 Apr-Jun;54(202):72-78. (PMID: 27935927)
PLoS One. 2014 Oct 31;9(10):e111491. (PMID: 25360633)
PLoS One. 2018 Nov 15;13(11):e0207478. (PMID: 30440048)
Jundishapur J Microbiol. 2016 Jan 02;9(1):e30682. (PMID: 27099694)
Int J Microbiol. 2015;2015:572163. (PMID: 26587024)
BMC Proc. 2019 Dec 16;13(Suppl 11):20. (PMID: 31890013)
EMBO Mol Med. 2017 Apr;9(4):430-447. (PMID: 28202493)
J Antimicrob Chemother. 2009 Mar;63(3):427-37. (PMID: 19155227)
Antimicrob Resist Infect Control. 2012 Jun 06;1(1):23. (PMID: 22958481)
Open Microbiol J. 2017 Apr 28;11:23-30. (PMID: 28553414)
Clin Infect Dis. 2005 Dec 1;41(11):1549-56. (PMID: 16267725)
Emerg Infect Dis. 2020 Jun;26(6):1156-1163. (PMID: 32267827)
Antimicrob Agents Chemother. 2010 Oct;54(10):4201-7. (PMID: 20660684)
Gut Pathog. 2012 Jul 16;4(1):7. (PMID: 22800501)
Antimicrob Resist Infect Control. 2015 Oct 26;4:42. (PMID: 26504518)
Am J Clin Pathol. 2012 Apr;137(4):627-32. (PMID: 22431540)
BMC Res Notes. 2009 Mar 26;2:49. (PMID: 19323805)
Antimicrob Resist Infect Control. 2017 Jun 15;6:67. (PMID: 28638594)

Keywords: Klebsiella pneumoniae; bla KPC; Antibiotic resistant; Carbapenemase

EC 3.5.2.6 (beta-Lactamases)
0 (Bacterial Proteins)
EC 3.5.2.6 (carbapenemase)
0 (Anti-Bacterial Agents)
FV9J3JU8B1 (Meropenem)

Date Created: 20240425 Date Completed: 20240425 Latest Revision: 20240429

20250114

PMC11044503

10.1186/s12866-024-03301-9

38664608