Cereal leaf beetle-associated bacteria enhance the survival of their host upon insecticide treatments and respond differently to insecticides with different modes of action.

Publisher: Society for Applied Microbiology and Blackwell Pub Country of Publication: United States NLM ID: 101499207 Publication Model: Print Cited Medium: Internet ISSN: 1758-2229 (Electronic) Linking ISSN: 17582229 NLM ISO Abbreviation: Environ Microbiol Rep Subsets: MEDLINE

Original Publication: [Hoboken, N.J.] : Society for Applied Microbiology and Blackwell Pub., c2009-

Insecticides*/pharmacology ; Bacteria*/genetics ; Bacteria*/classification ; Bacteria*/drug effects ; Bacteria*/isolation & purification ; Larva*/microbiology ; Larva*/drug effects ; Coleoptera*/microbiology ; Coleoptera*/drug effects; Animals ; RNA, Ribosomal, 16S/genetics ; Microbiota/drug effects ; Metagenomics ; Pyrethrins/pharmacology ; Chlorpyrifos ; Pantoea/genetics ; Pantoea/drug effects

The cereal leaf beetle (CLB, Oulema melanopus) is one of the major cereal pests. The effect of insecticides belonging to different chemical classes, with different mechanisms of action and the active substances' concentrations on the CLB bacterial microbiome, was investigated. Targeted metagenomic analysis of the V3-V4 regions of the 16S ribosomal gene was used to determine the composition of the CLB bacterial microbiome. Each of the insecticides caused a decrease in the abundance of bacteria of the genus Pantoea, and an increase in the abundance of bacteria of the genus Stenotrophomonas, Acinetobacter, compared to untreated insects. After cypermethrin application, a decrease in the relative abundance of bacteria of the genus Pseudomonas was noted. The dominant bacterial genera in cypermethrin-treated larvae were Lactococcus, Pantoea, while in insects exposed to chlorpyrifos or flonicamid it was Pseudomonas. Insecticide-treated larvae were characterized, on average, by higher biodiversity and richness of bacterial genera, compared to untreated insects. The depletion of CLB-associated bacteria resulted in a decrease in larval survival, especially after cypermethrin and chlorpyrifos treatments. The use of a metagenome-based functional prediction approach revealed a higher predicted function of bacterial acetyl-CoA C-acetyltransferase in flonicamid and chlorpyrifos-treated larvae and tRNA dimethyltransferase in cypermethrin-treated insects than in untreated insects.
(© 2024 The Authors. Environmental Microbiology Reports published by John Wiley & Sons Ltd.)

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UMO-2020/37/N/NZ9/02577 Polish National Science Centre

0 (Insecticides)
0 (RNA, Ribosomal, 16S)
0 (Pyrethrins)
JCS58I644W (Chlorpyrifos)
1TR49121NP (cypermethrin)

Date Created: 20240421 Date Completed: 20240423 Latest Revision: 20240426

20240426

PMC11033208

10.1111/1758-2229.13247

38644048