Lifelong persistence of nuclear RNAs in the mouse brain.

Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE

Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-

Brain*/metabolism ; Chromatin* ; RNA, Nuclear*/genetics; Animals ; Mice ; Gene Expression Regulation ; Heterochromatin/genetics

Genomic DNA that resides in the nuclei of mammalian neurons can be as old as the organism itself. The life span of nuclear RNAs, which are critical for proper chromatin architecture and transcription regulation, has not been determined in adult tissues. In this work, we identified and characterized nuclear RNAs that do not turn over for at least 2 years in a subset of postnatally born cells in the mouse brain. These long-lived RNAs were stably retained in nuclei in a neural cell type-specific manner and were required for the maintenance of heterochromatin. Thus, the life span of neural cells may depend on both the molecular longevity of DNA for the storage of genetic information and also the extreme stability of RNA for the functional organization of chromatin.

Comment in: Science. 2024 Apr 5;384(6691):31-32. (PMID: 38574156)

Dev Biol. 1977 Jun;57(2):417-26. (PMID: 873053)
Elife. 2017 Aug 01;6:. (PMID: 28760201)
Science. 2018 Apr 13;360(6385):176-182. (PMID: 29545511)
Methods. 2014 May 1;67(1):55-63. (PMID: 23872059)
Cell. 2019 Apr 18;177(3):654-668.e15. (PMID: 30929900)
Nat Neurosci. 2018 Feb;21(2):290-299. (PMID: 29335606)
Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308)
Cell. 1975 Jan;4(1):69-75. (PMID: 1078787)
Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15779-84. (PMID: 18840688)
STAR Protoc. 2021 Apr 16;2(2):100472. (PMID: 33948565)
Cell. 2013 Aug 29;154(5):971-982. (PMID: 23993091)
Front Neuroanat. 2015 May 07;9:53. (PMID: 25999820)
Nat Genet. 2011 Mar;43(3):220-7. (PMID: 21317888)
Bioinformatics. 2014 Apr 1;30(7):923-30. (PMID: 24227677)
Science. 2011 Feb 4;331(6017):593-6. (PMID: 21233348)
Sci Rep. 2017 Jul 21;7(1):6137. (PMID: 28733588)
Nat Methods. 2017 Jul;14(7):687-690. (PMID: 28581496)
Nat Rev Mol Cell Biol. 2022 Apr;23(4):231-249. (PMID: 35013589)
Cell Biochem Biophys. 2003;37(3):213-34. (PMID: 12625628)
STAR Protoc. 2021 Aug 25;2(3):100651. (PMID: 34485932)
Mol Cell. 2020 Jan 16;77(2):368-383.e7. (PMID: 31677973)
Nat Rev Mol Cell Biol. 2021 Oct;22(10):653-670. (PMID: 34341548)
Nature. 2011 Sep 07;477(7363):179-84. (PMID: 21901007)
Science. 2012 Feb 24;335(6071):942. (PMID: 22300851)
Cell. 2014 Dec 18;159(7):1698-710. (PMID: 25497548)
Cell. 2013 Jun 6;153(6):1219-1227. (PMID: 23746839)
Nat Med. 1998 Nov;4(11):1313-7. (PMID: 9809557)
Mol Cell. 2018 Jun 7;70(5):842-853.e7. (PMID: 29861157)
Cell Stem Cell. 2017 Nov 2;21(5):618-634.e7. (PMID: 28919367)
Elife. 2017 Aug 01;6:. (PMID: 28760199)
Dev Cell. 2010 Oct 19;19(4):625-38. (PMID: 20951352)
Nat Commun. 2022 Jun 20;13(1):3525. (PMID: 35725842)
Mol Biosyst. 2016 Feb;12(2):477-9. (PMID: 26661513)
Genes Dev. 2013 Aug 15;27(16):1769-86. (PMID: 23964093)

804468 International ERC_ European Research Council

0 (Chromatin)
0 (Heterochromatin)
0 (RNA, Nuclear)

Date Created: 20240404 Date Completed: 20240408 Latest Revision: 20240427

20240427

PMC7615865

10.1126/science.adf3481

38574132