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The Nucleocapsid Protein of SARS-CoV-2, Combined with ODN-39M, Is a Potential Component for an Intranasal Bivalent Vaccine with Broader Functionality.
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- معلومة اضافية
- المصدر:
Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
- بيانات النشر:
Original Publication: Basel, Switzerland : MDPI
- الموضوع:
- نبذة مختصرة :
Despite the rapid development of vaccines against COVID-19, they have important limitations, such as safety issues, the scope of their efficacy, and the induction of mucosal immunity. The present study proposes a potential component for a new generation of vaccines. The recombinant nucleocapsid (N) protein from the SARS-CoV-2 Delta variant was combined with the ODN-39M, a synthetic 39 mer unmethylated cytosine-phosphate-guanine oligodeoxynucleotide (CpG ODN), used as an adjuvant. The evaluation of its immunogenicity in Balb/C mice revealed that only administration by intranasal route induced a systemic cross-reactive, cell-mediated immunity (CMI). In turn, this combination was able to induce anti-N IgA in the lungs, which, along with the specific IgG in sera and CMI in the spleen, was cross-reactive against the nucleocapsid protein of SARS-CoV-1. Furthermore, the nasal administration of the N + ODN-39M preparation, combined with RBD Delta protein, enhanced the local and systemic immune response against RBD, with a neutralizing capacity. Results make the N + ODN-39M preparation a suitable component for a future intranasal vaccine with broader functionality against Sarbecoviruses.
- References:
Emerg Microbes Infect. 2021 Dec;10(1):629-637. (PMID: 33691606)
Vaccine. 2022 Feb 16;40(8):1162-1169. (PMID: 35078661)
Science. 2021 Feb 12;371(6530):735-741. (PMID: 33436524)
Hum Immunol. 2002 Dec;63(12):1111-9. (PMID: 12480254)
Vaccine. 2021 Nov 16;39(47):6920-6929. (PMID: 34696934)
JAMA. 2021 Jul 13;326(2):118-120. (PMID: 34160567)
Front Immunol. 2020 Nov 30;11:611337. (PMID: 33329607)
Cell. 2020 Aug 6;182(3):713-721.e9. (PMID: 32778225)
Arch Virol. 2020 Mar;165(3):593-607. (PMID: 32016547)
Sci Immunol. 2021 Dec 10;6(66):eabj5129. (PMID: 34890255)
Int J Pharm. 2019 Dec 15;572:118813. (PMID: 31678521)
J Virol. 2020 Jun 16;94(13):. (PMID: 32546606)
Vaccines (Basel). 2022 Apr 29;10(5):. (PMID: 35632456)
Vaccines (Basel). 2022 Mar 26;10(4):. (PMID: 35455265)
J Virol. 2010 Apr;84(7):3134-46. (PMID: 19906932)
Microbes Infect. 2020 May - Jun;22(4-5):188-194. (PMID: 32302675)
Sci Rep. 2023 Nov 7;13(1):19328. (PMID: 37935801)
EClinicalMedicine. 2022 Apr;46:101383. (PMID: 35434578)
Cell. 2021 Feb 18;184(4):861-880. (PMID: 33497610)
Cell Biosci. 2021 Dec 8;11(1):202. (PMID: 34879865)
Emerg Microbes Infect. 2022 Dec;11(1):1058-1071. (PMID: 35311493)
JAMA. 2021 Jul 6;326(1):35-45. (PMID: 34037666)
J Gen Virol. 2004 Aug;85(Pt 8):2407-2419. (PMID: 15269383)
Biosci Rep. 2021 Sep 30;41(9):. (PMID: 34519332)
Rev Infect Dis. 1989 May-Jun;11(3):494-7. (PMID: 2665004)
PLoS Biol. 2021 Oct 11;19(10):e3001425. (PMID: 34634033)
J Immunol. 2016 Nov 1;197(9):3597-3606. (PMID: 27683751)
N Engl J Med. 2022 Apr 21;386(16):1532-1546. (PMID: 35249272)
Cell. 2021 Dec 9;184(25):6037-6051.e14. (PMID: 34852237)
Vaccines (Basel). 2021 Jul 09;9(7):. (PMID: 34358183)
Immunol Cell Biol. 2015 Jan;93(1):57-66. (PMID: 25178969)
AIDS Res Hum Retroviruses. 2004 Nov;20(11):1269-81. (PMID: 15588349)
J Immunol. 2021 Jul 15;207(2):376-379. (PMID: 34193597)
N Engl J Med. 2022 Jan 27;386(4):297-299. (PMID: 34910863)
Nat Commun. 2022 Jan 10;13(1):80. (PMID: 35013199)
Sci Rep. 2021 Jul 19;11(1):14723. (PMID: 34282196)
Int Immunol. 2015 Aug;27(8):367-79. (PMID: 25795768)
Biochem Biophys Res Commun. 2021 Mar 5;543:45-49. (PMID: 33515911)
Expert Rev Vaccines. 2011 Apr;10(4):499-511. (PMID: 21506647)
J Exp Med. 2021 Apr 5;218(4):. (PMID: 33464307)
Vaccines (Basel). 2022 Dec 07;10(12):. (PMID: 36560498)
Scand J Immunol. 2021 Nov;94(5):e13100. (PMID: 34940992)
Emerg Top Life Sci. 2020 Dec 11;4(4):353-369. (PMID: 33258903)
J Clin Microbiol. 2021 Sep 20;59(10):e0052721. (PMID: 34288726)
Vaccine. 2014 Nov 12;32(48):6377-89. (PMID: 24975812)
Viral Immunol. 2010 Oct;23(5):521-9. (PMID: 20883166)
Eur J Epidemiol. 2021 Dec;36(12):1237-1240. (PMID: 34591202)
N Engl J Med. 2021 Aug 12;385(7):585-594. (PMID: 34289274)
Nature. 2020 Aug;584(7821):463-469. (PMID: 32717743)
Emerg Microbes Infect. 2021 Dec;10(1):1751-1759. (PMID: 34396940)
Science. 2020 Aug 14;369(6505):806-811. (PMID: 32434945)
Nat Commun. 2020 Nov 30;11(1):6121. (PMID: 33257686)
Eur Phys J Plus. 2023;138(2):157. (PMID: 36811098)
J Virol. 2020 Aug 31;94(18):. (PMID: 32661140)
PLoS One. 2014 Feb 24;9(2):e88846. (PMID: 24586411)
MEDICC Rev. 2022 Jan 31;24(1):14-18. (PMID: 35157634)
Vaccine. 2021 Apr 15;39(16):2280-2287. (PMID: 33731271)
Front Microbiol. 2021 Nov 05;12:752739. (PMID: 34803974)
Cell Res. 2022 Jan;32(1):107-109. (PMID: 34862467)
N Engl J Med. 2020 Dec 17;383(25):2439-2450. (PMID: 33053279)
Nat Protoc. 2020 Nov;15(11):3699-3715. (PMID: 32978602)
Cell Rep. 2021 Sep 7;36(10):109664. (PMID: 34450033)
Cell. 2022 Mar 3;185(5):896-915.e19. (PMID: 35180381)
- Grant Information:
2021YFE0192200 Ministry of Science and Technology of the People's Republic of China; 2019CB1012 Hunan Provincial Base for Scientific and Technological Innovation Cooperation; 2020RC5035 The Science and Technology Innovation Program of Hunan Province; 2020WK2031 Hunan Provincial Innovative Construction Program
- Contributed Indexing:
Keywords: ODN; SARS-CoV-2; bivalent vaccine; broad vaccine; cell-mediated immunity; intranasal; neutralizing Abs
- الرقم المعرف:
0 (Nucleocapsid Proteins)
0 (Vaccines, Combined)
0 (COVID-19 Vaccines)
0 (Vaccines)
0 (Adjuvants, Immunologic)
0 (Antibodies, Viral)
0 (Antibodies, Neutralizing)
- الموضوع:
SARS-CoV-2 variants
- الموضوع:
Date Created: 20240328 Date Completed: 20240329 Latest Revision: 20240522
- الموضوع:
20240522
- الرقم المعرف:
PMC10976088
- الرقم المعرف:
10.3390/v16030418
- الرقم المعرف:
38543783
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