Knockdown of DNMT1 Induces SLCO3A1 to Promote Follicular Growth by Enhancing the Proliferation of Granulosa Cells in Mammals .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Granulosa Cells*/metabolism ; Ovarian Follicle*/metabolism; Mice ; Female ; Swine ; Animals ; Cell Proliferation/genetics ; Mammals/genetics ; RNA, Messenger/genetics

In female mammals, the proliferation and apoptosis of granulosa cells (GCs) have been shown to determine the fate of follicles. DNA methyltransferases (DNMTs) and SLCO3A1 have been reported to be involved in the survival of GCs and follicular growth. However, the molecular mechanisms enabling DNMTs to regulate the expression of SLCO3A1 to participate in follicular growth are unclear. In this study, we found that the knockdown of DNMT1 enhanced the mRNA and protein levels of SLCO3A1 by regulating the chromatin accessibility probably. Moreover, SLCO3A1 upregulated the mRNA and protein levels of MCL1 , PCNA, and STAR to promote the proliferation of GCs and facilitated cell cycle progression by increasing the mRNA and protein levels of CCNE1 , CDK2, and CCND1 , but it decreased apoptosis by downregulating the mRNA and protein levels of CASP3 and CASP8 . Moreover, SLCO3A1 promoted the growth of porcine follicles and development of mice follicles. In conclusion, the knockdown of DNMT1 upregulated the mRNA and protein levels of SLCO3A1 , thereby promoting the proliferation of GCs to facilitate the growth and development of ovarian follicles, and these results provide new insights into investigations of female reproductive diseases.

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2023A1515030054 Guangdong Basic and Applied Basic Research Foundation; 2023A1515010364 Guangdong Basic and Applied Basic Research Foundation; 31902131 National Natural Science Foundation of China; 32072694 National Natural Science Foundation of China; CARS-35 Earmarked fund for China Agriculture Research System; 2022B0202090002 Key R&D Program of Guangdong Province Project; 2022-4408X1-43010402-0019 Breed Industry Innovation Park of Guangdong Xiaoerhua Pig

Keywords: DNMT1; SLCO3A1; follicular growth; proliferation

0 (RNA, Messenger)

Date Created: 20240313 Date Completed: 20240314 Latest Revision: 20240315

20240315

PMC10930803

10.3390/ijms25052468

38473715