Novel mechanisms of diversity generation in Acinetobacter baumannii resistance islands driven by Tn7-like elements.

Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.

Acinetobacter baumannii*/genetics ; DNA Transposable Elements*/genetics ; Drug Resistance, Bacterial*/genetics ; Genetic Variation*/genetics ; Genomic Islands*/genetics; Escherichia coli/genetics ; Genome, Bacterial/genetics

Mobile genetic elements play an important role in the acquisition of antibiotic and biocide resistance, especially through the formation of resistance islands in bacterial chromosomes. We analyzed the contribution of Tn7-like transposons to island formation and diversification in the nosocomial pathogen Acinetobacter baumannii and identified four separate families that recognize different integration sites. One integration site is within the comM gene and coincides with the previously described Tn6022 elements suggested to account for the AbaR resistance island. We established Tn6022 in a heterologous E. coli host and confirmed basic features of transposition into the comM attachment site and the use of a novel transposition protein. By analyzing population features within Tn6022 elements we identified two potential novel transposon-encoded diversification mechanisms with this dynamic genetic island. The activities of these diversification features were confirmed in E. coli. One was a novel natural gain-of-activity allele that could function to broaden transposition targeting. The second was a transposon-encoded hybrid dif-like site that parasitizes the host dimer chromosome resolution system to function with its own tyrosine recombinase. This work establishes a highly active Tn7-like transposon that harnesses novel features allowing the spread and diversification of genetic islands in pathogenic bacteria.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)

Science. 2019 Jul 05;365(6448):48-53. (PMID: 31171706)
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7358-E7366. (PMID: 28811374)
Microb Genom. 2023 Mar;9(3):. (PMID: 36880881)
J Mol Biol. 2001 Jan 19;305(3):633-42. (PMID: 11152618)
Proc Natl Acad Sci U S A. 2013 May 28;110(22):E2038-45. (PMID: 23674682)
mBio. 2021 Dec 21;12(6):e0293821. (PMID: 34872347)
Nucleic Acids Res. 2012 Feb;40(3):e19. (PMID: 22123741)
Nucleic Acids Res. 2019 Jan 8;47(D1):D427-D432. (PMID: 30357350)
Science. 1996 Apr 19;272(5260):401-4. (PMID: 8602527)
Nature. 1982 Jun 17;297(5867):601-3. (PMID: 6283361)
Nat Biotechnol. 2021 Apr;39(4):480-489. (PMID: 33230293)
Genes Dev. 2001 Mar 15;15(6):737-47. (PMID: 11274058)
Microbiol Spectr. 2014 Oct;2(5):. (PMID: 26104363)
Front Microbiol. 2018 Jan 26;9:66. (PMID: 29434581)
Genes Dev. 1996 Sep 1;10(17):2145-57. (PMID: 8804309)
Cell. 2020 Dec 23;183(7):1757-1771.e18. (PMID: 33271061)
Bioinformatics. 2014 May 1;30(9):1312-3. (PMID: 24451623)
Gene. 1994 Jan 28;138(1-2):1-7. (PMID: 8125283)
Nature. 2022 Sep;609(7926):384-393. (PMID: 36002573)
EMBO J. 1996 Nov 15;15(22):6348-61. (PMID: 8947057)
Nature. 2023 Jan;613(7945):775-782. (PMID: 36442503)
J Mol Biol. 1989 May 5;207(1):35-52. (PMID: 2544738)
Genetics. 1997 Mar;145(3):573-85. (PMID: 9055068)
Nat Struct Mol Biol. 2022 Feb;29(2):143-151. (PMID: 35173349)
Trends Biochem Sci. 1998 Dec;23(12):486-90. (PMID: 9868372)
Cell. 2021 Apr 29;184(9):2441-2453.e18. (PMID: 33770501)
J Bacteriol. 1988 Jan;170(1):352-8. (PMID: 2826397)
Genetics. 2008 Jul;179(3):1237-50. (PMID: 18562643)
EMBO J. 2003 Nov 3;22(21):5904-17. (PMID: 14592987)
Nature. 2021 Aug;596(7873):583-589. (PMID: 34265844)
Plasmid. 2009 Jan;61(1):1-14. (PMID: 18951916)
Microbiology (Reading). 2001 Dec;147(Pt 12):3241-7. (PMID: 11739756)
Antimicrob Agents Chemother. 2019 Mar 27;63(4):. (PMID: 30917986)
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296. (PMID: 33885785)
Protein Sci. 2021 Jan;30(1):70-82. (PMID: 32881101)
Nucleic Acids Res. 2015 Dec 15;43(22):10734-45. (PMID: 26384427)
Nucleic Acids Res. 2018 Jul 2;46(W1):W200-W204. (PMID: 29905871)
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. (PMID: 10829079)
J Bacteriol. 2007 Mar;189(5):2170-3. (PMID: 17194796)
Gene. 1995 May 26;158(1):9-14. (PMID: 7789817)
J Antimicrob Chemother. 2010 Jun;65(6):1162-70. (PMID: 20375036)
J Antimicrob Chemother. 2011 Nov;66(11):2484-91. (PMID: 21873287)
Cell. 1993 Mar 26;72(6):931-43. (PMID: 8384534)
Mol Cell. 2022 Feb 03;82(3):616-628.e5. (PMID: 35051352)
Mol Microbiol. 2019 Dec;112(6):1635-1644. (PMID: 31502713)
Genome Res. 2004 Jun;14(6):1188-90. (PMID: 15173120)
J Mol Biol. 1996 Mar 29;257(2):301-16. (PMID: 8609625)
Nucleic Acids Res. 2023 Jan 25;51(2):765-782. (PMID: 36537206)
Mol Gen Genet. 1984;194(1-2):149-58. (PMID: 6328211)
BMC Bioinformatics. 2004 Aug 19;5:113. (PMID: 15318951)
Bioinformatics. 2010 Mar 1;26(5):680-2. (PMID: 20053844)
Mol Cell. 2023 Jun 1;83(11):1827-1838.e6. (PMID: 37267904)
mSphere. 2020 May 27;5(3):. (PMID: 32461273)
Nucleic Acids Res. 2022 Jun 24;50(11):6368-6383. (PMID: 35657090)
PLoS One. 2009 Sep 03;4(9):e6531. (PMID: 19727445)
PLoS One. 2010 Mar 10;5(3):e9490. (PMID: 20224823)
Plasmid. 2006 Mar;55(2):152-7. (PMID: 16139359)
Mol Microbiol. 2014 Sep;93(6):1084-92. (PMID: 25091064)
Cell. 1978 Dec;15(4):1199-208. (PMID: 728998)
Nature. 2019 Jul;571(7764):219-225. (PMID: 31189177)
Mol Cell. 2000 Sep;6(3):573-82. (PMID: 11030337)
Genes Dev. 1988 Feb;2(2):137-49. (PMID: 2834269)
Antimicrob Agents Chemother. 2020 Sep 21;64(10):. (PMID: 32778544)
Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. (PMID: 37267947)
EMBO J. 2004 Aug 4;23(15):2972-81. (PMID: 15257292)
Plasmid. 1996 Jan;35(1):1-13. (PMID: 8693022)
Bioinformatics. 2014 Jul 15;30(14):2068-9. (PMID: 24642063)
Microb Genom. 2016 Feb 23;2(2):e000052. (PMID: 28348844)
J Bacteriol. 1995 Jul;177(14):4121-30. (PMID: 7608087)
Bioinformatics. 2011 Apr 1;27(7):1009-10. (PMID: 21278367)

R01 GM129118 United States GM NIGMS NIH HHS; R21 AI148941 United States AI NIAID NIH HHS; R01 GM129118 United States NH NIH HHS

0 (DNA Transposable Elements)

Date Created: 20240226 Date Completed: 20240415 Latest Revision: 20240425

20240425

PMC11014353

10.1093/nar/gkae129

38407477