Protective Effect of Betulin on Streptozotocin-Nicotinamide-Induced Diabetes in Female Rats.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Diabetes Mellitus, Experimental*/chemically induced ; Diabetes Mellitus, Type 2*/drug therapy ; Betulinic Acid*; Rats ; Female ; Animals ; Antioxidants/therapeutic use ; Niacinamide/pharmacology ; Niacinamide/therapeutic use ; Rats, Wistar ; Streptozocin/adverse effects ; Blood Glucose ; Plant Extracts/pharmacology ; Hypoglycemic Agents/adverse effects ; Glucose/adverse effects ; Biomarkers ; alpha-Amylases

Type 2 diabetes is characterized by hyperglycemia and a relative loss of β-cell function. Our research investigated the antidiabetic potential of betulin, a pentacyclic triterpenoid found primarily in birch bark and, intriguingly, in a few marine organisms. Betulin has been shown to possess diverse biological activities, including antioxidant and antidiabetic activities; however, no studies have fully explored the effects of betulin on the pancreas and pancreatic islets. In this study, we investigated the effect of betulin on streptozotocin-nicotinamide (STZ)-induced diabetes in female Wistar rats. Betulin was prepared as an emulsion, and intragastric treatments were administered at doses of 20 and 50 mg/kg for 28 days. The effect of treatment was assessed by analyzing glucose parameters such as fasting blood glucose, hemoglobin A1C, and glucose tolerance; hepatic and renal biomarkers; lipid peroxidation; antioxidant enzymes; immunohistochemical analysis; and hematological indices. Administration of betulin improved the glycemic response and decreased α-amylase activity in diabetic rats, although insulin levels and homeostatic model assessment for insulin resistance (HOMA-IR) scores remained unchanged. Furthermore, betulin lowered the levels of hepatic biomarkers (aspartate aminotransferase, alanine aminotransferase, and alpha-amylase activities) and renal biomarkers (urea and creatine), in addition to improving glutathione levels and preventing the elevation of lipid peroxidation in diabetic animals. We also found that betulin promoted the regeneration of β-cells in a dose-dependent manner but did not have toxic effects on the pancreas. In conclusion, betulin at a dose of 50 mg/kg exerts a pronounced protective effect against cytolysis, diabetic nephropathy, and damage to the acinar pancreas and may be a potential treatment option for diabetes.

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Prioгity-2030 Program Ministry of Science and Higher Education of the Russian Federation; ED431C2018/05 European Regional European Funds; № 122020900136-4 Institute of Immunology and Physiology UB Russian Academy of Science (IIP UB RAS)

Keywords: antidiabetic; betulin; insulin; islet regeneration; nicotinamide; streptozotocin; type 2 diabetes

0 (Antioxidants)
25X51I8RD4 (Niacinamide)
5W494URQ81 (Streptozocin)
0 (Blood Glucose)
0 (Plant Extracts)
6W70HN7X7O (betulin)
0 (Hypoglycemic Agents)
IY9XDZ35W2 (Glucose)
0 (Biomarkers)
EC 3.2.1.1 (alpha-Amylases)
4G6A18707N (Betulinic Acid)

Date Created: 20240224 Date Completed: 20240226 Latest Revision: 20240227

20240227

PMC10888537

10.3390/ijms25042166

38396842