Cooperative control of a DNA origami force sensor.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Nanostructures*/chemistry; Nucleic Acid Conformation ; DNA/chemistry ; Mechanical Phenomena ; Oligonucleotides ; Microscopy, Atomic Force/methods

Biomolecular systems are dependent on a complex interplay of forces. Modern force spectroscopy techniques provide means of interrogating these forces, but they are not optimized for studies in constrained environments as they require attachment to micron-scale probes such as beads or cantilevers. Nanomechanical devices are a promising alternative, but this requires versatile designs that can be tuned to respond to a wide range of forces. We investigate the properties of a nanoscale force sensitive DNA origami device which is highly customizable in geometry, functionalization, and mechanical properties. The device, referred to as the NanoDyn, has a binary (open or closed) response to an applied force by undergoing a reversible structural transition. The transition force is tuned with minor alterations of 1 to 3 DNA oligonucleotides and spans tens of picoNewtons (pN). The DNA oligonucleotide design parameters also strongly influence the efficiency of resetting the initial state, with higher stability devices (≳10 pN) resetting more reliably during repeated force-loading cycles. Finally, we show the opening force is tunable in real time by adding a single DNA oligonucleotide. These results establish the potential of the NanoDyn as a versatile force sensor and provide fundamental insights into how design parameters modulate mechanical and dynamic properties.
(© 2024. The Author(s).)

Update of: bioRxiv. 2023 Jun 28;:. (PMID: 37425797)

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P30 CA016058 United States CA NCI NIH HHS; R35 GM139564 United States GM NIGMS NIH HHS

9007-49-2 (DNA)
0 (Oligonucleotides)

Date Created: 20240219 Date Completed: 20240221 Latest Revision: 20240304

20240304

PMC10876929

10.1038/s41598-024-53841-3

38374280