Hydrogel-mediated extracellular vesicles for enhanced wound healing: the latest progress, and their prospects for 3D bioprinting.

Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE

Original Publication: London : BioMed Central, 2003-

Hydrogels* ; Bioprinting*/methods; Wound Healing

Extracellular vesicles have shown promising tissue recovery-promoting effects, making them increasingly sought-after for their therapeutic potential in wound treatment. However, traditional extracellular vesicle applications suffer from limitations such as rapid degradation and short maintenance during wound administration. To address these challenges, a growing body of research highlights the role of hydrogels as effective carriers for sustained extracellular vesicle release, thereby facilitating wound healing. The combination of extracellular vesicles with hydrogels and the development of 3D bioprinting create composite hydrogel systems boasting excellent mechanical properties and biological activity, presenting a novel approach to wound healing and skin dressing. This comprehensive review explores the remarkable mechanical properties of hydrogels, specifically suited for loading extracellular vesicles. We delve into the diverse sources of extracellular vesicles and hydrogels, analyzing their integration within composite hydrogel formulations for wound treatment. Different composite methods as well as 3D bioprinting, adapted to varying conditions and construction strategies, are examined for their roles in promoting wound healing. The results highlight the potential of extracellular vesicle-laden hydrogels as advanced therapeutic tools in the field of wound treatment, offering both mechanical support and bioactive functions. By providing an in-depth examination of the various roles that these composite hydrogels can play in wound healing, this review sheds light on the promising directions for further research and development. Finally, we address the challenges associated with the application of composite hydrogels, along with emerging trends of 3D bioprinting in this domain. The discussion covers issues such as scalability, regulatory considerations, and the translation of this technology into practical clinical settings. In conclusion, this review underlines the significant contributions of hydrogel-mediated extracellular vesicle therapy to the field of 3D bioprinting and wound healing and tissue regeneration. It serves as a valuable resource for researchers and practitioners alike, fostering a deeper understanding of the potential benefits, applications, and challenges involved in utilizing composite hydrogels for wound treatment.
(© 2024. The Author(s).)

Bioact Mater. 2021 Jul 05;8:296-308. (PMID: 34541402)
Int J Mol Sci. 2019 Sep 18;20(18):. (PMID: 31540457)
Stem Cell Res Ther. 2022 Aug 8;13(1):407. (PMID: 35941707)
Trends Biotechnol. 2018 Sep;36(9):907-922. (PMID: 29764691)
Theranostics. 2019 Jan 1;9(1):65-76. (PMID: 30662554)
Biotechnol Adv. 2020 Jul - Aug;41:107549. (PMID: 32302653)
J Mater Chem B. 2016 Nov 14;4(42):6830-6841. (PMID: 32263577)
Biomacromolecules. 2021 May 10;22(5):1921-1931. (PMID: 33840195)
Small. 2011 Jun 6;7(11):1569-78. (PMID: 21538871)
ACS Nano. 2019 Aug 27;13(8):8694-8707. (PMID: 31390518)
J Biomed Mater Res A. 2020 Nov 1;108(11):2138-2149. (PMID: 32319166)
Adv Wound Care (New Rochelle). 2018 Feb 1;7(2):29-45. (PMID: 29392092)
PLoS Med. 2009 Feb 17;6(2):e1000029. (PMID: 19226183)
Biomater Adv. 2022 Aug;139:213000. (PMID: 35891601)
Int J Bioprint. 2023 Jan 17;9(2):670. (PMID: 37065655)
Cell Prolif. 2022 Apr;55(4):e13196. (PMID: 35156747)
Nanomaterials (Basel). 2020 Jul 29;10(8):. (PMID: 32751101)
ACS Nano. 2021 Feb 23;15(2):3212-3227. (PMID: 33470092)
Biomaterials. 2004 Aug;25(18):4263-71. (PMID: 15046916)
Cell Death Differ. 2005 Jul;12(7):695-8. (PMID: 15861190)
Regen Med. 2014;9(4):497-512. (PMID: 25159066)
Pharmaceutics. 2021 Apr 08;13(4):. (PMID: 33918073)
Bioact Mater. 2022 Feb 28;16:271-284. (PMID: 35386320)
Stem Cells Transl Med. 2017 Mar;6(3):736-747. (PMID: 28297576)
Cell Transplant. 2011;20(2):205-16. (PMID: 20719083)
Adv Healthc Mater. 2018 Apr;7(8):e1701161. (PMID: 29283220)
Surg Clin North Am. 1976 Aug;56(4):859-74. (PMID: 959948)
Nat Commun. 2023 Nov 29;14(1):7856. (PMID: 38030636)
Biomaterials. 2010 Jan;31(2):308-14. (PMID: 19782393)
Materials (Basel). 2021 Sep 09;14(18):. (PMID: 34576409)
J Nanobiotechnology. 2022 Mar 19;20(1):147. (PMID: 35305648)
ACS Biomater Sci Eng. 2023 Jul 10;9(7):3945-3952. (PMID: 33882674)
Mater Today Bio. 2020 Jun 27;7:100067. (PMID: 32695985)
J Invest Dermatol. 2008 Oct;128(10):2526-40. (PMID: 18449211)
Biomaterials. 2015 Dec;73:254-71. (PMID: 26414409)
ACS Appl Mater Interfaces. 2019 Oct 16;11(41):37421-37433. (PMID: 31525863)
Adv Drug Deliv Rev. 2013 Apr;65(4):457-70. (PMID: 23137786)
J Extracell Vesicles. 2022 Jun;11(6):e12238. (PMID: 35716060)
Pharmaceutics. 2023 Jan 22;15(2):. (PMID: 36839705)
Adv Drug Deliv Rev. 2021 Jul;174:348-368. (PMID: 33964356)
Mater Today Bio. 2022 Jul 16;16:100365. (PMID: 35967739)
Acta Biomater. 2022 Jul 15;147:342-355. (PMID: 35580827)
Int J Nanomedicine. 2020 Aug 11;15:5911-5926. (PMID: 32848396)
Sci Adv. 2021 May 12;7(20):. (PMID: 33980490)
ACS Nano. 2019 Sep 24;13(9):10279-10293. (PMID: 31483606)
Curr Diab Rep. 2016 Mar;16(3):29. (PMID: 26897744)
Front Bioeng Biotechnol. 2019 Mar 22;7:45. (PMID: 30968018)
Nat Rev Mater. 2016 Dec;1(12):. (PMID: 29657852)
Polymers (Basel). 2020 Sep 03;12(9):. (PMID: 32899245)
Int J Endocrinol. 2015;2015:848272. (PMID: 26089898)
J Mater Chem B. 2022 Jun 8;10(22):4242-4253. (PMID: 35579559)
Acta Biomater. 2022 Dec;154:212-230. (PMID: 36309190)
J Biomater Sci Polym Ed. 2015;26(7):433-45. (PMID: 25661399)
Carbohydr Polym. 2019 Dec 1;225:115249. (PMID: 31521268)
Biomaterials. 2020 Aug;249:120020. (PMID: 32305816)
Acta Mater. 2013 Feb 1;61(3):912-930. (PMID: 23457423)
Pharmaceutics. 2022 Feb 21;14(2):. (PMID: 35214197)
Biochemistry. 2018 Aug 14;57(32):4867-4879. (PMID: 30001488)
Nat Biotechnol. 2014 Aug;32(8):773-85. (PMID: 25093879)
Sci Adv. 2022 Jul 8;8(27):eabn7006. (PMID: 35857459)
Tissue Eng Part A. 2021 Sep;27(17-18):1168-1181. (PMID: 33218292)
Biomolecules. 2021 May 08;11(5):. (PMID: 34066746)
Int J Biol Macromol. 2021 Oct 1;188:820-832. (PMID: 34371046)
J Dermatol Sci. 2021 Feb;101(2):123-133. (PMID: 33358097)
Bioact Mater. 2021 Feb 18;6(9):2829-2840. (PMID: 33718665)
Mater Today Bio. 2023 Nov 13;23:100863. (PMID: 38089434)
Acta Biomater. 2020 Feb;103:24-51. (PMID: 31805409)
J Mater Chem B. 2023 Jan 25;11(4):837-851. (PMID: 36594635)
Front Physiol. 2017 Oct 23;8:818. (PMID: 29109687)
Bioengineering (Basel). 2020 Apr 29;7(2):. (PMID: 32365578)
Adv Funct Mater. 2012 May 23;22(10):2027-2039. (PMID: 22907987)
Acta Biomater. 2023 Feb;157:175-186. (PMID: 36503078)
Br J Dermatol. 2019 Oct;181(4):854-856. (PMID: 30953591)
Angiogenesis. 2015 Apr;18(2):191-200. (PMID: 25537851)
J Biomed Mater Res A. 2020 Mar;108(3):545-556. (PMID: 31702867)
Mol Ther. 2006 Dec;14(6):840-50. (PMID: 16965940)
Int J Nanomedicine. 2021 Nov 30;16:7831-7846. (PMID: 34876812)
Nat Rev Mol Cell Biol. 2018 Apr;19(4):213-228. (PMID: 29339798)
Adv Wound Care (New Rochelle). 2015 Mar 1;4(3):119-136. (PMID: 25785236)
ACS Nano. 2021 Apr 27;15(4):6352-6368. (PMID: 33723994)
Biomaterials. 2018 Jun;168:38-53. (PMID: 29614431)
Nanoscale. 2022 Jun 16;14(23):8525-8533. (PMID: 35660804)
Adv Drug Deliv Rev. 2020 Jan 1;153:28-53. (PMID: 31678360)
Int J Mol Sci. 2020 Dec 18;21(24):. (PMID: 33352826)
J Control Release. 2020 Dec 10;328:532-550. (PMID: 32971198)
Pharmaceutics. 2022 Sep 19;14(9):. (PMID: 36145727)
J Invest Dermatol. 2016 Sep;136(9):1885-1891. (PMID: 27287183)
Theranostics. 2022 Jun 21;12(11):4879-4903. (PMID: 35836815)
Adv Sci (Weinh). 2023 Sep;10(26):e2303650. (PMID: 37424038)
Microb Biotechnol. 2019 Jul;12(4):586-610. (PMID: 30838788)
Med Res Rev. 2022 Nov;42(6):2102-2125. (PMID: 35757979)
Bioact Mater. 2021 Jun 22;8:505-514. (PMID: 34541416)
Stem Cells Dev. 2016 Jul 15;25(14):1073-83. (PMID: 27226274)
Stem Cells Transl Med. 2015 May;4(5):513-22. (PMID: 25824139)
Stem Cell Res Ther. 2022 Jul 15;13(1):314. (PMID: 35841007)
Small. 2022 Apr;18(15):e2200060. (PMID: 35229462)
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110611. (PMID: 32228999)
ACS Nano. 2020 Apr 28;14(4):4444-4455. (PMID: 32282185)
Trends Biotechnol. 2023 Nov;41(11):1343-1359. (PMID: 37302911)
Adv Healthc Mater. 2016 Feb 4;5(3):326-33. (PMID: 26626828)
J Mater Chem B. 2020 Aug 19;8(32):7197-7212. (PMID: 32633312)
Carbohydr Polym. 2019 Oct 15;222:114988. (PMID: 31320082)
Int J Mol Sci. 2022 Jun 03;23(11):. (PMID: 35682968)
Bioconjug Chem. 2019 Apr 17;30(4):994-1005. (PMID: 30855944)
Biomater Sci. 2018 May 1;6(5):915-946. (PMID: 29492503)
Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126721. (PMID: 37673168)
Trends Biotechnol. 2020 Oct;38(10):1066-1098. (PMID: 32564882)
Adv Drug Deliv Rev. 2018 Jul;132:270-295. (PMID: 30055210)
Signal Transduct Target Ther. 2021 Feb 17;6(1):66. (PMID: 33594043)
Biomater Sci. 2019 Dec 17;8(1):313-324. (PMID: 31701966)
Biomater Adv. 2022 Feb;133:112613. (PMID: 35527135)
Int J Inflam. 2019 Jun 2;2019:3706315. (PMID: 31275545)
Small. 2022 Jan;18(1):e2104229. (PMID: 34791802)
Int J Mol Sci. 2022 Jan 01;23(1):. (PMID: 35008902)
Drug Deliv. 2020 Dec;27(1):1308-1318. (PMID: 32924637)
Adv Healthc Mater. 2022 Mar;11(5):e2100538. (PMID: 34310074)
Adv Ther. 2017 Mar;34(3):599-610. (PMID: 28108895)
Chem Rev. 2020 Oct 14;120(19):10695-10743. (PMID: 32323975)
Tissue Cell. 2023 Dec;85:102213. (PMID: 37666183)
Adv Healthc Mater. 2024 Feb;13(4):e2302394. (PMID: 37950552)
ACS Omega. 2023 Mar 08;8(11):10030-10039. (PMID: 36969465)
Stem Cells Dev. 2014 Jun 1;23(11):1233-44. (PMID: 24367916)
Biomaterials. 2018 Nov;183:185-199. (PMID: 30172244)
Bioact Mater. 2023 Jun 27;28:511-536. (PMID: 37435177)
Adv Mater. 2023 Jun;35(23):e2210903. (PMID: 36916986)
J Mol Histol. 2020 Jun;51(3):251-263. (PMID: 32388839)
Arch Dermatol Res. 2022 Mar;314(2):133-140. (PMID: 33641015)
J Biol Chem. 2014 Aug 8;289(32):22258-67. (PMID: 24951588)
Mater Today Bio. 2022 Sep 24;16:100427. (PMID: 36193344)
Front Bioeng Biotechnol. 2022 Aug 23;10:903779. (PMID: 36082169)
Int J Biol Macromol. 2022 Dec 1;222(Pt A):1453-1464. (PMID: 36113600)
Carbohydr Polym. 2020 May 15;236:116025. (PMID: 32172843)
Plast Reconstr Surg Glob Open. 2015 Feb 06;3(1):e284. (PMID: 25674365)
Nat Biomed Eng. 2020 Apr;4(4):370-380. (PMID: 31695178)
Nat Methods. 2021 Sep;18(9):1013-1026. (PMID: 34446922)
J Biomed Mater Res A. 2022 Jun;110(6):1190-1198. (PMID: 35080115)
FASEB J. 2018 Jan;32(1):512-528. (PMID: 28935818)
J Funct Biomater. 2014 Sep 18;5(3):197-210. (PMID: 25238548)
Adv Healthc Mater. 2022 Aug;11(16):e2200494. (PMID: 35751637)
Biomed Res Int. 2013;2013:916136. (PMID: 23984420)
Biomed Mater. 2011 Aug;6(4):045002. (PMID: 21613722)
Bioact Mater. 2023 Mar 15;26:323-336. (PMID: 36950152)
Adv Drug Deliv Rev. 2019 Jun;146:37-59. (PMID: 30172924)
Eur J Pharm Biopharm. 2022 Feb;171:50-59. (PMID: 34793943)
J Transl Med. 2015 Feb 01;13:49. (PMID: 25638205)
Wound Repair Regen. 2016 Sep;24(5):759-766. (PMID: 27256925)
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Sep-Oct;2(5):510-25. (PMID: 20607703)
Biosens Bioelectron. 2021 Mar 1;175:112849. (PMID: 33250333)
Front Bioeng Biotechnol. 2021 Feb 18;9:650598. (PMID: 33681176)
Int J Biol Macromol. 2018 Oct 1;117:102-107. (PMID: 29772339)
Biomed Res Int. 2013;2013:385641. (PMID: 23653894)
Cells. 2020 Jul 21;9(7):. (PMID: 32708202)
Adv Mater. 2014 Jan 8;26(1):85-123. (PMID: 24741694)
Int J Mol Sci. 2016 Dec 11;17(12):. (PMID: 27973441)
Front Cell Dev Biol. 2021 May 03;9:570179. (PMID: 34012958)
Immunol Cell Biol. 2019 Mar;97(3):258-267. (PMID: 30746824)
Biofabrication. 2020 May 28;12(3):035018. (PMID: 32252038)
Tissue Eng Part B Rev. 2012 Aug;18(4):301-11. (PMID: 22429320)

82272289 National Natural Science Foundation of China; 822CXTD537 Hainan Province National Science Foundation of China; 21YF1424300 Shanghai Sailing program

Keywords: 3D bioprinting; Extracellular vesicle; Hydrogels; Regenerative medicine; Would healing

0 (Hydrogels)

Date Created: 20240210 Date Completed: 20240214 Latest Revision: 20240214

20250114

PMC10858484

10.1186/s12951-024-02315-9

38341585