Effect of Selected Antioxidants on the In Vitro Aging of Human Fibroblasts.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Antioxidants*/pharmacology ; Antioxidants*/metabolism ; Ergothioneine*/metabolism ; Cyclic N-Oxides* ; Spin Labels*; Humans ; Resveratrol/pharmacology ; Resveratrol/metabolism ; Coumaric Acids/pharmacology ; Fibroblasts/metabolism ; Reactive Oxygen Species/metabolism ; Oxidative Stress

The modification of the replicative lifespan (RLS) of fibroblasts is of interest both from a knowledge point of view and for the attenuation of skin aging. The effect of six antioxidants at a concentration of 1 μM on the replicative lifespan of human dermal fibroblasts was studied. The nitroxide 4-hydroxy-TEMPO (TEMPOL), ergothioneine, and Trolox extended the replicative lifespan (RLS) (40 ± 1 population doublings (PD)) by 7 ± 2, 4 ± 1, and 3 ± 1 PD and lowered the expression of p21 at late passages. Coumaric acid, curcumin and resveratrol did not affect the RLS . The level of reactive oxygen species (ROS) was decreased or not affected by the antioxidants although TEMPOL and coumaric acid decreased the level of glutathione. Only ergothioneine and resveratrol decreased the level of protein carbonylation. The antioxidants that could prolong the RLS elevated the mitochondrial membrane potential. Protecting the activity of mitochondria seems to be important for maintaining the replicative capacity of fibroblasts.

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2015/17/B/NZ3/03731 National Science Center

Keywords: 4-hydroxy-TEMPO; Trolox; antioxidant; ergothioneine; fibroblast; in vitro aging; mitochondrial membrane potential; p21

0 (Antioxidants)
U78ZX2F65X (tempol)
BDZ3DQM98W (Ergothioneine)
Q369O8926L (Resveratrol)
0 (Coumaric Acids)
0 (Reactive Oxygen Species)
0 (Cyclic N-Oxides)
0 (Spin Labels)

Date Created: 20240210 Date Completed: 20240214 Latest Revision: 20240214

20250114

PMC10855218

10.3390/ijms25031529

38338809