Left ventricle function and post-transcriptional events with exercise training in pigs.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Heart Ventricles* ; MicroRNAs*/genetics; Swine ; Animals ; Ventricular Function, Left ; Diastole ; Myocardium

Background: Standardized exercise protocols have been shown to improve overall cardiovascular fitness, but direct effects on left ventricular (LV) function, particularly diastolic function and relation to post-transcriptional molecular pathways (microRNAs (miRs)) are poorly understood. This project tested the central hypothesis that adaptive LV remodeling resulting from a large animal exercise training protocol, would be directly associated with specific miRs responsible for regulating pathways relevant to LV myocardial stiffness and geometry.
Methods and Results: Pigs (n = 9; 25 Kg) underwent a 4 week exercise training protocol (10 degrees elevation, 2.5 mph, 10 min, 5 days/week) whereby LV chamber stiffness (KC) and regional myocardial stiffness (rKm) were measured by Doppler/speckle tracking echocardiography. Age and weight matched non-exercise pigs (n = 6) served as controls. LV KC fell by approximately 50% and rKm by 30% following exercise (both p < 0.05). Using an 84 miR array, 34 (40%) miRs changed with exercise, whereby 8 of the changed miRs (miR-19a, miR-22, miR-30e, miR-99a, miR-142, miR-144, miR-199a, and miR-497) were correlated to the change in KC (r ≥ 0.5 p < 0.05) and mapped to matrix and calcium handling processes. Additionally, miR-22 and miR-30e decreased with exercise and mapped to a localized inflammatory process, the inflammasome (NLRP-3, whereby a 2-fold decrease in NLRP-3 mRNA occurred with exercise (p < 0.05).
Conclusion: Chronic exercise reduced LV chamber and myocardial stiffness and was correlated to miRs that map to myocardial relaxation processes as well as local inflammatory pathways. These unique findings set the stage for utilization of myocardial miR profiling to identify underlying mechanisms by which exercise causes changes in LV myocardial structure and function.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Samani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Am J Physiol Heart Circ Physiol. 2010 Nov;299(5):H1348-56. (PMID: 20817828)
Int J Sports Med. 2022 Mar;43(3):219-229. (PMID: 34416779)
Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2735-40. (PMID: 19193853)
Am Heart J. 2011 Dec;162(6):1003-10. (PMID: 22137073)
Medicina (Kaunas). 2019 Apr 15;55(4):. (PMID: 30991661)
Med Sci Sports Exerc. 2019 Jun;51(6):1099-1105. (PMID: 30640284)
Nature. 2005 Feb 17;433(7027):769-73. (PMID: 15685193)
Mol Med Rep. 2017 Nov;16(5):7287-7296. (PMID: 28944921)
JAMA. 2000 Jun 21;283(23):3095-101. (PMID: 10865304)
Circulation. 2018 Nov 27;138(22):2530-2544. (PMID: 30571348)
J Clin Ultrasound. 2022 Oct;50(8):1026-1040. (PMID: 36218206)
Cell Death Differ. 2017 Jul;24(7):1205-1213. (PMID: 26160071)
Am J Physiol Heart Circ Physiol. 2018 Mar 1;314(3):H627-H642. (PMID: 29167118)
Circulation. 2002 Mar 19;105(11):1387-93. (PMID: 11901053)
Circ Res. 2019 Oct 11;125(9):787-801. (PMID: 31434553)
Front Genet. 2020 Mar 18;11:256. (PMID: 32256529)
Oxid Med Cell Longev. 2020 Feb 17;2020:4063562. (PMID: 32148650)
Br Heart J. 1971 Jul;33(4):616. (PMID: 5557501)
J Cardiopulm Rehabil Prev. 2019 Mar;39(2):118-126. (PMID: 30624371)
Physiol Genomics. 2011 Jun 15;43(11):665-73. (PMID: 21447748)
Circulation. 2018 Apr 10;137(15):1549-1560. (PMID: 29311053)
Bioinformatics. 2014 Feb 15;30(4):523-30. (PMID: 24336805)
Ann Rheum Dis. 1999 Nov;58(11):691-7. (PMID: 10531073)
Front Physiol. 2013 Apr 11;4:80. (PMID: 23596423)
J Appl Physiol (1985). 2006 Jul;101(1):40-6. (PMID: 16565350)
Scand J Med Sci Sports. 2018 Nov;28(11):2330-2338. (PMID: 29968944)
Clin Sci (Lond). 2016 Nov 1;130(22):2005-2015. (PMID: 27503950)
Clin Sci (Lond). 2019 Sep 10;133(17):1845-1856. (PMID: 31434695)
Nucleic Acids Res. 2020 Jul 2;48(W1):W244-W251. (PMID: 32484539)
Antioxid Redox Signal. 2017 Jul 1;27(1):1-20. (PMID: 27502441)
Biol Trace Elem Res. 2021 Dec;199(12):4593-4603. (PMID: 33439455)
Circulation. 2021 Sep 21;144(12):934-946. (PMID: 34543068)
J Cardiovasc Transl Res. 2013 Aug;6(4):501-15. (PMID: 23716130)
J Am Coll Cardiol. 2016 May 3;67(17):2050-60. (PMID: 27126533)
J Am Coll Cardiol. 2021 Oct 5;78(14):1453-1470. (PMID: 34593128)
Eur Heart J Cardiovasc Imaging. 2018 Jun 1;19(6):591-600. (PMID: 29596561)
J Anim Sci. 1973 May;36(5):927-30. (PMID: 4703721)
J Hum Hypertens. 2010 Mar;24(3):175-82. (PMID: 19554028)
J Pineal Res. 2019 Apr;66(3):e12552. (PMID: 30618087)
J Am Coll Cardiol. 2019 Apr 30;73(16):2062-2072. (PMID: 31023430)
Exp Ther Med. 2018 Oct;16(4):3703-3709. (PMID: 30233729)
J Am Coll Cardiol. 2019 May 7;73(17):2209-2225. (PMID: 31047010)
Equine Vet J. 2021 May;53(3):618-627. (PMID: 32484928)
Am J Physiol Heart Circ Physiol. 2011 Oct;301(4):H1579-87. (PMID: 21856915)
PLoS One. 2015 Apr 16;10(4):e0122107. (PMID: 25881132)
Front Physiol. 2020 May 08;11:372. (PMID: 32477155)
Int J Clin Exp Pathol. 2015 Apr 01;8(4):3994-4000. (PMID: 26097586)
Br Heart J. 1980 Mar;43(3):284-92. (PMID: 7437175)
Microvasc Res. 2021 Nov;138:104212. (PMID: 34175330)
J Am Coll Cardiol. 2011 Oct 18;58(17):1780-91. (PMID: 21996391)
Trials. 2015 Mar 12;16:90. (PMID: 25872588)
J Physiol. 2011 Aug 15;589(Pt 16):3983-94. (PMID: 21690193)
Nat Rev Immunol. 2019 Aug;19(8):477-489. (PMID: 31036962)
J Mol Cell Cardiol. 2014 Dec;77:168-74. (PMID: 25451387)
Biol Pharm Bull. 2019;42(4):518-523. (PMID: 30930410)
Lab Invest. 2021 Mar;101(3):369-380. (PMID: 33268842)
Cells. 2021 Dec 09;10(12):. (PMID: 34943988)
J Am Coll Cardiol. 1988 Feb;11(2):317-21. (PMID: 3339169)
J Appl Physiol (1985). 2005 Apr;98(4):1154-62. (PMID: 15772055)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Biomed Res Int. 2014;2014:985408. (PMID: 25013816)
Cell Calcium. 2017 Jan;61:10-21. (PMID: 27720444)
JAMA. 2009 Apr 8;301(14):1439-50. (PMID: 19351941)
Vet J. 2016 Feb;208:22-7. (PMID: 26701817)
Clin Chim Acta. 2019 Dec;499:98-107. (PMID: 31499022)
JACC Basic Transl Sci. 2020 Apr 29;5(5):463-480. (PMID: 32478208)
Am J Physiol Heart Circ Physiol. 2018 Aug 1;315(2):H273-H283. (PMID: 29600898)
Eur J Prev Cardiol. 2021 Dec 29;28(15):1673-1681. (PMID: 33742210)
Circ Res. 2012 Jan 6;110(1):126-44. (PMID: 22223210)
J Cell Biol. 2001 Dec 10;155(6):1017-27. (PMID: 11739411)
Am J Physiol Heart Circ Physiol. 2018 Oct 1;315(4):H958-H967. (PMID: 30004234)
Sci Rep. 2019 May 17;9(1):7527. (PMID: 31101833)
ESC Heart Fail. 2021 Jun;8(3):1850-1860. (PMID: 33830643)
Oncotarget. 2020 Jun 02;11(22):2106-2119. (PMID: 32547708)
Am J Physiol Heart Circ Physiol. 2011 Oct;301(4):H1687-94. (PMID: 21841018)
Am J Cardiol. 1986 Feb 15;57(6):450-8. (PMID: 2936235)
J Biol Chem. 2010 Oct 1;285(40):30804-13. (PMID: 20663871)
Genes Nutr. 2020 Aug 27;15(1):15. (PMID: 32854610)
Am J Physiol Heart Circ Physiol. 2018 Nov 1;315(5):H1443-H1452. (PMID: 30141982)
Front Genet. 2016 Nov 15;7:196. (PMID: 27895662)
J Appl Physiol (1985). 2014 Mar 1;116(5):522-31. (PMID: 24436293)

I01 BX000168 United States BX BLRD VA; I01 BX005320 United States BX BLRD VA; R01 HL130972 United States HL NHLBI NIH HHS

0 (MicroRNAs)

Date Created: 20240202 Date Completed: 20240205 Latest Revision: 20240210

20240210

PMC10836705

10.1371/journal.pone.0292243

38306359