Transcriptomic analyses of Vibrio parahaemolyticus under the phenyllactic acid stress.

Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE

Original Publication: Berlin ; New York : Springer International, c1984-

Vibrio parahaemolyticus*/genetics ; Lactates*; Gene Expression Profiling ; Anti-Bacterial Agents/pharmacology ; Polyesters

Phenyllactic acid (PLA) generally recognized as a natural organic acid shows against Vibrio parahaemolyticus activity. In this study, V. parahaemolyticus ATCC17802 (Vp17802) was cultured under the stress of 1/2MIC PLA, and then the antibacterial mechanisms were explored via transcriptomics. The minimum inhibitory concentration (MIC) of PLA against Vp17802 was 3.2 mg/mL, and the time-kill analysis resulted that Vp17802 was inhibited. PLA was able to destroy the bacterial membrane, leading to the leakage of intracellular substances and decline of ATP levels. The RNA-sequencing analysis results indicated that 1616 significantly differentially expressed genes were identified, among which 190 were up-regulated and 1426 were down-regulated. Down-regulation of the icd2 gene in the TCA cycle mediates blockage of tyrosine metabolic, arginine biosynthesis, and oxidative phosphorylation, causing insufficient energy supply of Vp17802. Moreover, PLA could cause amino acids, metal ions, and phosphate transporters to be blocked, affecting the acquisition of nutrients. The treatment by PLA altered the expression of genes encoding functions involved in quorum sensing, flagellar assembly, and cell chemotaxis pathway, which may be interfering with the biofilm formation in Vp17802, reducing cell motility. Overall, 1.6 mg/mL PLA inhibited the growth of Vp17802 by disrupting to uptake of nutrients, cell metabolism, and the formation of biofilms. The results suggested a new direction for exploring the activity of PLA against Vp17802 and provided a theoretical basis for bacterial pathogen control in the food industry. KEY POINTS: •RNA sequencing was carried out to indicate the antibacterial mechanism of Vp17802. •The icd2 gene in the TCA cycle mediates blockage of metabolic of Vp17802. •The biofilm formation has interfered with 1.6 mg/mL PLA, which could reduce cell motility and virulence.
(© 2024. The Author(s).)

Front Microbiol. 2021 Apr 23;12:664281. (PMID: 33968001)
Food Microbiol. 2016 Aug;57:128-34. (PMID: 27052711)
J Struct Biol. 2009 Nov;168(2):313-22. (PMID: 19646531)
Future Microbiol. 2019 Aug;14:1043-1053. (PMID: 31469011)
J Food Prot. 2019 Dec;82(12):2016-2022. (PMID: 31692394)
J Food Prot. 1998 Oct;61(10):1281-5. (PMID: 9798142)
Int J Food Microbiol. 2014 Mar 03;173:30-5. (PMID: 24412414)
Arch Microbiol. 2017 Aug;199(6):917-929. (PMID: 28378142)
J Appl Microbiol. 2022 Jan;132(1):101-112. (PMID: 34091972)
Mol Microbiol. 2011 Jan;79(1):240-63. (PMID: 21166906)
J Basic Microbiol. 2016 Sep;56(9):975-85. (PMID: 27282849)
Food Chem. 2017 Aug 1;228:533-540. (PMID: 28317760)
Molecules. 2019 Feb 20;24(4):. (PMID: 30791538)
BMC Biochem. 2005 Sep 29;6:20. (PMID: 16194279)
mSystems. 2022 Dec 20;7(6):e0064922. (PMID: 36286553)
Food Res Int. 2021 Jan;139:109562. (PMID: 33509445)
Microbiol Res. 2019 May;222:43-51. (PMID: 30928029)
Annu Rev Food Sci Technol. 2012;3:381-403. (PMID: 22385168)
Front Vet Sci. 2017 Aug 10;4:126. (PMID: 28848739)
Nat Rev Microbiol. 2022 Mar;20(3):161-173. (PMID: 34548639)
Front Cell Infect Microbiol. 2018 Nov 16;8:389. (PMID: 30505805)
PLoS Genet. 2019 May 23;15(5):e1008145. (PMID: 31120900)
Foods. 2021 Apr 24;10(5):. (PMID: 33923242)
J Biol Chem. 2019 Oct 18;294(42):15505-15516. (PMID: 31484721)
Int J Food Microbiol. 2022 May 16;369:109606. (PMID: 35287031)
Lancet. 2003 Mar 1;361(9359):743-9. (PMID: 12620739)
FEMS Microbiol Lett. 2002 Feb 19;208(1):83-7. (PMID: 11934498)

2019B020212003 R&D Projects in Key Areas of Guangdong Province; 2021B0202060001 R&D Projects in Key Areas of Guangdong Province; XT202303 Collaborative Innovation Center of GDAAS; 2022DZX018 Jieyang Science and Technology Project; 202206010177 Science and Technology Program of Guangzhou

Keywords: Antibacterial; Phenyllactic acid; Transcriptomics; Vibrio parahaemolyticus

156-05-8 (3-phenyllactic acid)
0 (Anti-Bacterial Agents)
0 (Polyesters)
0 (Lactates)

Date Created: 20240129 Date Completed: 20240130 Latest Revision: 20241023

20250114

PMC10824802

10.1007/s00253-024-13024-6

38285117