A Comprehensive Phytochemical Analysis of Sideritis scardica Infusion Using Orbitrap UHPLC-HRMS.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Sideritis* ; Lamiaceae*; Chromatography, High Pressure Liquid ; Carboxylic Acids ; Iridoid Glycosides ; Tea

Sideritis scardica Griseb, also known as "mountain tea" and "Olympus tea" (Lamiaceae family) is an endemic plant from the mountainous regions of the Balkan Peninsula. In this study, we focused on an in-depth phytochemical analysis of S. scardica infusion using ultra-high-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UHPLC-HRMS). Quantitative determination of the main secondary metabolites was carried out by UHPLC-HRMS analyses using the external standard method. The results revealed more than 100 metabolites, including five sugar acids and saccharides, 21 carboxylic, hydroxybenzoic, hydroxycinnamic acids, and derivatives, 15 acylquinic acids, 10 phenylpropanoid glycosides, four iridoid glycosides, 28 flavonoids, seven fatty acids, and four organosulfur compounds. Furthermore, a dereplication and fragmentation patterns of five caffeic acids oligomers and four acylhexaric acids was performed for the first time in S. scardica . Regarding the quantitative analysis, the phenylethanoid verbascoside ( 53 ) (151.54 ± 10.86 mg/g lyophilized infusion, li), the glycosides of isoscutellarein ( 78 ) (151.70 ± 14.78 mg/g li), methylisoscutelarein ( 82 ) (107.4 ± 9.07 mg/g li), and hypolaetin ( 79 ) (78.33 ± 3.29 mg/g li), as well as caffeic acid ( 20 ) (87.25 ± 6.54 mg/g li), were found to be the major compounds in S. scardica infusion. The performed state-of-the-art phytochemical analysis of S. scardica provides additional knowledge for the chemical constituents and usage of this valuable medicinal plant.

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BG-RRP-2.004-0004-C01 European Union-NextGenerationEU, through the National Recovery and Resilience Plan of the Republic of Bulgaria

Keywords: Sideritis scardica Griseb; UHPLC–HRMS; phytochemical analysis

0 (Carboxylic Acids)
0 (Iridoid Glycosides)
0 (Tea)

Date Created: 20240111 Date Completed: 20240112 Latest Revision: 20240112

20240112

PMC10780595

10.3390/molecules29010204

38202787