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PRMT2 silencing regulates macrophage polarization through activation of STAT1 or inhibition of STAT6.

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  • المؤلفون: Liu T;Liu T; Li Y; Li Y; Xu M; Xu M; Huang H; Huang H; Luo Y; Luo Y
  • المصدر:
    BMC immunology [BMC Immunol] 2024 Jan 03; Vol. 25 (1), pp. 1. Date of Electronic Publication: 2024 Jan 03.
  • نوع النشر :
    Journal Article; Research Support, Non-U.S. Gov't
  • اللغة:
    English
  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100966980 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2172 (Electronic) Linking ISSN: 14712172 NLM ISO Abbreviation: BMC Immunol Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central, [2000-
    • الموضوع:
      Interleukin-4*/metabolism ; Lipopolysaccharides*/pharmacology ; Lipopolysaccharides*/metabolism; Animals ; Mice ; Inflammation/metabolism ; Interferon-gamma/metabolism ; Macrophage Activation ; Macrophages ; Signal Transduction ; STAT6 Transcription Factor/metabolism
    • نبذة مختصرة :
      Background: Macrophages play significant roles in innate immune responses and are heterogeneous cells that can be polarized into M1 or M2 phenotypes. PRMT2 is one of the type I protein arginine methyltransferases involved in inflammation. However, the role of PRMT2 in M1/M2 macrophage polarization remains unclear. Our study revealed the effect and mechanism of PRMT2 in macrophage polarization.
      Methods: Bone marrow-derived macrophages (BMDMs) were polarized to M1 or M2 state by LPS plus murine recombinant interferon-γ (IFN-γ) or interleukin-4 (IL-4). Quantitative polymerase chain reaction (qPCR), western blot and flow cytometry (FCM) assay were performed and analyzed markers and signaling pathways of macrophage polarization.
      Results: We found that PRMT2 was obviously upregulated in LPS/IFN-γ-induced M1 macrophages, but it was little changed in IL-4-induced M2 macrophages. Furthermore, PRMT2 konckdown increased the expression of M1 macrophages markers through activation of STAT1 and decreased the expression of M2 macrophages markers through inhibition of STAT6.
      Conclusions: PRMT2 silencing modulates macrophage polarization by activating STAT1 to promote M1 and inhibiting STAT6 to attenuate the M2 state.
      (© 2023. The Author(s).)
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    • Grant Information:
      No. 82102259 Yinjiao Li; No. 82101840 Hongjun Huang; No. 81871101 Yan Luo
    • Contributed Indexing:
      Keywords: M1/M2; Macrophage polarization; PRMT2; STAT1/STAT6
    • الرقم المعرف:
      82115-62-6 (Interferon-gamma)
      207137-56-2 (Interleukin-4)
      0 (Lipopolysaccharides)
      0 (STAT6 Transcription Factor)
      EC 2.1.1.319 (PRMT2 protein, mouse)
      0 (Stat1 protein, mouse)
      0 (Stat6 protein, mouse)
    • الموضوع:
      Date Created: 20240103 Date Completed: 20240110 Latest Revision: 20240229
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC10765854
    • الرقم المعرف:
      10.1186/s12865-023-00593-w
    • الرقم المعرف:
      38172698