The follicle-stimulating hormone triggers rapid changes in mitochondrial structure and function in porcine cumulus cells.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Follicle Stimulating Hormone*/pharmacology ; Follicle Stimulating Hormone*/metabolism ; Cumulus Cells*/metabolism; Pregnancy ; Swine ; Female ; Animals ; Oocytes/metabolism ; Oogenesis ; Follicle Stimulating Hormone, Human/metabolism ; Mitochondria ; Meiosis

Oocyte maturation is a key process during which the female germ cell undergoes resumption of meiosis and completes its preparation for embryonic development including cytoplasmic and epigenetic maturation. The cumulus cells directly surrounding the oocyte are involved in this process by transferring essential metabolites, such as pyruvate, to the oocyte. This process is controlled by cyclic adenosine monophosphate (cAMP)-dependent mechanisms recruited downstream of follicle-stimulating hormone (FSH) signaling in cumulus cells. As mitochondria have a critical but poorly understood contribution to this process, we defined the effects of FSH and high cAMP concentrations on mitochondrial dynamics and function in porcine cumulus cells. During in vitro maturation (IVM) of cumulus-oocyte complexes (COCs), we observed an FSH-dependent mitochondrial elongation shortly after stimulation that led to mitochondrial fragmentation 24 h later. Importantly, mitochondrial elongation was accompanied by decreased mitochondrial activity and a switch to glycolysis. During a pre-IVM culture step increasing intracellular cAMP, mitochondrial fragmentation was prevented. Altogether, the results demonstrate that FSH triggers rapid changes in mitochondrial structure and function in COCs involving cAMP.
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9002-68-0 (Follicle Stimulating Hormone)
0 (Follicle Stimulating Hormone, Human)

Date Created: 20240103 Date Completed: 20240105 Latest Revision: 20240108

20240108

PMC10764925

10.1038/s41598-023-50586-3

38172520