Evidence of the Beneficial Impact of Three Probiotic-Based Food Supplements on the Composition and Metabolic Activity of the Intestinal Microbiota in Healthy Individuals: An Ex Vivo Study.

Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI Publishing

Gastrointestinal Microbiome* ; Probiotics*/pharmacology ; Ammonium Compounds*; Humans ; Dietary Supplements ; Lactobacillus acidophilus/physiology ; Fatty Acids, Volatile

Scientific evidence has increasingly supported the beneficial effects of probiotic-based food supplements on human intestinal health. This ex vivo study investigated the effects on the composition and metabolic activity of the intestinal microbiota of three probiotic-based food supplements, containing, respectively, (1) Bifidobacterium longum ES1, (2) Lactobacillus acidophilus NCFM ^® , and (3) a combination of L. acidophilus NCFM ^® , Lactobacillus paracasei Lpc-37™, Bifidobacterium lactis Bi-07™, and Bifidobacterium lactis Bl-04™. This study employed fecal samples from six healthy donors, inoculated in a Colon-on-a-plate ^® system. After 48 h of exposure or non-exposure to the food supplements, the effects were measured on the overall microbial fermentation (pH), changes in microbial metabolic activity through the production of short-chain and branched-chain fatty acids (SCFAs and BCFAs), ammonium, lactate, and microbial composition. The strongest effect on the fermentation process was observed for the combined formulation probiotics, characterized by the significant stimulation of butyrate production, a significant reduction in BCFAs and ammonium in all donors, and a significant stimulatory effect on bifidobacteria and lactobacilli growth. Our findings suggest that the combined formulation probiotics significantly impact the intestinal microbiome of the healthy individuals, showing changes in metabolic activity and microbial abundance as the health benefit endpoint.

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Instituto Central Lechera Asturiana para la Nutrición Personalizada (ICLANP)

Keywords: Bifidobacterium longum ES1; Bifidobacterium spp.; HOWARU® restore; Lactobacillus acidophilus NCFM®; Lactobacillus spp.; ammonium; branched short-chain fatty acids (BCFA); lactate; probiotic-based food supplements; short-chain fatty acids (SCFA)

0 (Fatty Acids, Volatile)
0 (Ammonium Compounds)

Date Created: 20231223 Date Completed: 20231225 Latest Revision: 20231225

20231225

PMC10745619

10.3390/nu15245077

38140334