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Tn3-like structures co-harboring of bla CTX-M-65 , bla TEM-1 and bla OXA-10 in the plasmids of two Escherichia coli ST1508 strains originating from dairy cattle in China.

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  • معلومة اضافية
    • المصدر:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
    • بيانات النشر:
      Original Publication: London : BioMed Central, 2005-
    • الموضوع:
      Escherichia coli Infections*/veterinary ; Cattle Diseases*; Cattle ; Animals ; Escherichia coli ; Phylogeny ; Anti-Bacterial Agents/pharmacology ; beta-Lactamases/genetics ; Plasmids/genetics ; China
    • نبذة مختصرة :
      The purpose of this study was to determine the level of horizontal transmission of the bla CTX-M-65 gene and the role of its associated mobile genetic elements (MGEs) in the bovine-derived Escherichia coli. After PCR identification, two plasmids carrying bla CTX-M-65 were successfully transferred to the recipient E. coli J53 Azr through conjugation assays and subsequently selected for Whole-Genome sequencing (WGS) analysis. The resistance profiles of these two positive strains and their transconjugants were also determined through antimicrobial susceptibility tests. Whole genome data were acquired using both the PacBio sequencing platform and the Illumina data platform. The annotated results were then submitted to the Genbank database for accession number recording. For comparison, the genetic environment of plasmids carrying the resistance gene bla CTX-M-65 was mapped using the Easyfig software. WGS analysis revealed Tn3-like composite transposons bearing bla CTX-M-65 , bla TEM-1 , and bla OXA-10 in the IncHI2-type plasmids of these two E. coli ST1508 strains. A phylogenetic tree was generated from all 48 assembled E. coli isolates bla CTX-M-65 , bla TEM-1 , and bla OXA-10 from the NCBI Pathogen Detection database with our two isolates, showing the relationships and the contribution of SNPs to the diversity between genetic samples. This study suggests that the transmissibility of bla CTX-M-65 on Tn3-like composite transposons contributes to an increased risk of its transmission in E. coli derived from dairy cattle.
      (© 2023. The Author(s).)
    • References:
      Infect Drug Resist. 2022 Sep 08;15:5315-5320. (PMID: 36110130)
      J Antimicrob Chemother. 2017 Aug 1;72(8):2145-2155. (PMID: 28541467)
      Antimicrob Agents Chemother. 2005 Apr;49(4):1319-22. (PMID: 15793104)
      Front Microbiol. 2023 Jul 13;14:1197579. (PMID: 37520353)
      Vet Microbiol. 2003 Aug 29;95(1-2):103-9. (PMID: 12860080)
      Nat Rev Microbiol. 2005 Sep;3(9):722-32. (PMID: 16138100)
      Front Microbiol. 2012 Apr 02;3:110. (PMID: 22485109)
      Epidemiol Infect. 2001 Apr;126(2):173-9. (PMID: 11349966)
      Heliyon. 2020 Jan 30;6(1):e03206. (PMID: 32042963)
      mSphere. 2018 Jul 18;3(4):. (PMID: 30021873)
      Curr Opin Microbiol. 2006 Oct;9(5):466-75. (PMID: 16942899)
      J Glob Antimicrob Resist. 2019 Jun;17:216-220. (PMID: 30658198)
      J Food Prot. 2016 Jul;79(7):1169-73. (PMID: 27357036)
      Front Microbiol. 2021 Jul 20;12:653595. (PMID: 34354678)
      Genome Res. 2020 Jan;30(1):138-152. (PMID: 31809257)
      Microb Drug Resist. 2011 Mar;17(1):83-97. (PMID: 21281129)
      PLoS One. 2020 Sep 14;15(9):e0236442. (PMID: 32925914)
      Microb Pathog. 2020 Nov;148:104450. (PMID: 32853679)
      Int J Antimicrob Agents. 2012 Apr;39(4):305-10. (PMID: 22325120)
      FEMS Microbiol Rev. 2010 Nov;34(6):1015-36. (PMID: 20412308)
      Infect Drug Resist. 2020 Sep 30;13:3383-3391. (PMID: 33061485)
      PLoS One. 2015 Aug 11;10(8):e0135044. (PMID: 26263489)
      Front Microbiol. 2022 Sep 08;13:955827. (PMID: 36160190)
      Front Vet Sci. 2022 Aug 01;9:738904. (PMID: 35978707)
      PLoS One. 2014 Aug 11;9(8):e104984. (PMID: 25110940)
      J Appl Microbiol. 2016 Feb;120(2):379-87. (PMID: 26606545)
      Microb Drug Resist. 2016 Jun;22(4):301-11. (PMID: 26669767)
      JAC Antimicrob Resist. 2021 Jul 16;3(3):dlab092. (PMID: 34286272)
      Microbiol Spectr. 2015 Aug;3(4):. (PMID: 26350313)
      Front Microbiol. 2014 Dec 16;5:688. (PMID: 25566207)
      Bioinformatics. 2011 Apr 1;27(7):1009-10. (PMID: 21278367)
      Antimicrob Agents Chemother. 2018 Aug 27;62(9):. (PMID: 29967018)
    • Contributed Indexing:
      Keywords: Bla CTX-M-65; Dairy cattle; E. coli; Tn3-like; Transmission
    • الرقم المعرف:
      EC 3.5.2.- (beta-lactamase PSE-2)
      0 (Anti-Bacterial Agents)
      EC 3.5.2.6 (beta-Lactamases)
    • الموضوع:
      Date Created: 20231219 Date Completed: 20231220 Latest Revision: 20231221
    • الموضوع:
      20250114
    • الرقم المعرف:
      PMC10729465
    • الرقم المعرف:
      10.1186/s12917-023-03847-2
    • الرقم المعرف:
      38110972